Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Phase-Separation Properties of Poly(Ethylene Glycol) had Dextran Solutions In Microfluidic Device

미세 유체장치 내에서 Poly(Ethylene Glycol)과 Dextran 용액의 상 형성 특성 연구

  • 최주형 (인하대학교 생물공학과) ;
  • 장우진 (인하대학교 초정밀생물분리기술연구센터) ;
  • 이상우 (연세대학교 의공학부)
  • Published : 2007.04.30
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Abstract

Fluidic conditions for the separation of phases were surveyed in a microfluidic aqueous two-phase extraction system. The infusion ratio between polyethylene glycol (PEG) and dextran solution defines the concentrations of each polymer in micro-channel, which determine the phase-separation. The appropriate ratio between PEG (M.W. 8000, 10%, w/v) and dextran T500 (M.W. 500000, 5%, w/v) in order to perform the separation of phases of both polymers was observed as changing the mixed ratio of both polymers. Based on the fluidic conditions, stable two-phase solutions were obtained within 4% to 8% and 3% to 1% of PEG and dextran, respectively. In addition, the characteristics of the two-phase were discussed. The separation technique studied in the paper can be applied for the implementation of a lab-on-a chip which can detect various biological entities such cells, bacterium, and virus in an integrated manner using built in a biosensor inside the chip.

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