동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제21권5호
  • /
  • Pages.1278-1284
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  • 2007
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  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

Suppressive Effect of Solanum Iyratum Aqueous Extract Via Down-regulation of $TNF-{\alpha}$ and $IFN-{\gamma}$ Production on Collagen-induced Arthritis in Mice

  • Kim, Seung-Hyung (Institute of Traditional Medicine & Bioscience, Daejeon University) ;
  • Seo, Chang-Woo (Department of Herbology and Prescription, Sangji University) ;
  • Kim, Chang-Min (Department of Herbology and Prescription, Sangji University) ;
  • Kim, Yang-Jin (Department of Herbology and Prescription, Sangji University) ;
  • Lee, Boo-Kyun (Department of Herbology and Prescription, Sangji University) ;
  • Choi, Yong-Sun (Department of Herbology and Prescription, Sangji University) ;
  • Oh, Hwang (Department of Herbology and Prescription, Sangji University) ;
  • Yoon, Ho-Sok (Department of Herbology and Prescription, Sangji University) ;
  • Lee, Seon-Goo (Department of Pathology, College of Oriental Medicine, Sangji University) ;
  • Lee, Jang-Cheon (Department of Herbology and Prescription, Sangji University) ;
  • Lee, Young-Cheal (Department of Herbology and Prescription, Sangji University)
  • 발행 : 2007.10.25
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초록

Solanum Iyratum Thunb (Solanaceae) has multiple applications in korean traditional medicine because of its cytotoxic, immunological and anti-inflammatory activities. However, no study on the anti-arthritic activity of Solanum Iyratum Thunb has been reported in vivo. Rheumatoid arthritis (RA) is a systemic autoimmune disease with chronic inflammation characterized by hyperplasia of synovial cells in affected joints, which ultimately leads to the destruction of cartilage and bone. Cytokine production were assessed during CIA(collagen-induced arthritis) model mice in lymph node (LN), in knee joint and spleen, using ELISA. DBA/1j mice were immunized with bovine type II collagen. After a second collagen immunization, mice were treated with Solanum Iyratum Thunb (SLT) orally at 400, 200 mg/kg once a day for 4 weeks. The severity of arthritis within the knee joints was evaluated by histological assessment of cartilage destruction and pannus formation. SLT significantly suppressed the progression of CIA and inhibited the production of TNF-alpha and IFN-gamma in serum and spleen cell culture supernatant. The erosion of cartilage was dramatically reduced in mouse knees after treatment with SLT. In conclusion, our results demonstrates that SLT significantly suppressed the progression of CIA. This action was characterized by suppression of arthritis index, cartilage erosion and synovial cell infiltration and the decreased production of $TNF-{\alpha}$, $IFN-{\gamma}$, CD4+, CD19+, CD3+/CD69+ cells (in lymph node), CD11b+/Gr-1 + (in knee joint).

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