Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-apoptotic and Neuroprotective Effects of Bambusae Caulis in Liquamen Manufactured by Different Production Process

생산공정 차이에 따른 죽력(竹瀝)이 apoptosis 및 신경세포 보호 효과에 미치는 영향

  • Choi, Chan-Hun (Department of Oriental Physiology, College of Oriental Medicine, Dongshin University)
  • 최찬헌 (동신대학교 한의과대학 생리학교실)
  • Published : 2007.10.25
Download PDF
⟨ Previous Next ⟩

Abstract

Bambusae Caulis in Liquamen(BCL) has been commonly prescribed for stroke patients in the traditional Oriental medicine. So this study is aims to investigate the anti-apoptotic and neuroprotective effects of Bambusae Caulis in Liquamen(BCL) manufactured by different production process on the focal ischemia induced by intraluminal filament insertion in rats. The focal ischemia was induced by intraluminal filament insertion into middle cerebral artery. The animals were divided into four groups (n=15 in each group). The ischemia induced and not treated group : Control group, the ischemia induced and oral medication of the three kinds of BCL : BCL-A group, BCL-B group, BCL-C group. BCL-A was produced by heating at a low temperature$(250^{\circ} C)$ in electric kiln and filtering. BCL-B was produced by heating at a high temperature$(900^{\circ} C{\sim}1,000^{\circ}C)$ in yellow earth kiln and refining and filtering. BCL-C was produced by heating at a low temperature$(400^{\circ} C)$ yellow earth kiln and no refining and filtering. The anti-apoptotic and neuroprotective effects of the oral medication of BCL were observed by Bax, BCL-2, cytochrome c, mGluR5, cresyl violet and ChAT-stain. Our study suggests that BCl-A(was produced by heating at a low temperature in electric kiln and filtering) and BCL-C(was produced by heating at a low temperature in yellow earth kiln and no refining and filtering) show anti-apoptotic and neuroprotective effects on the focal ischemia induced by intraluminal filament insertion in rats and BCL-C is more effective than BCL-A.

Keywords

References

  1. 張伯臾. 中醫內科學. 上海, 上海科學技術出版社, p 212, 1988
  2. 洪性範. 高血壓 中風의 漢方療法. 서울, 醫藥社, p 305, 306, 1983
  3. 대한병리학회. 병리학. 서울, 고문사, p 33, 34, 1218, 1995
  4. 조연희, 김종근, 김경근, 김명규, 조기현, 김세종. 흰쥐 대뇌국소허혈시 BCL-2, BAX 및 ICE 유전자 발현의 변동. 대한신경과학회지 17(4):568-573, 1999
  5. 許浚. 東醫寶鑑. 서울, 南山堂, p 303, 1966
  6. 王浴生 外. 中藥藥理與應用. 北京, 人民衛生出版社, p 109, 198, 264, 424, 442, 460, 483, 723, 767, 853, 1983
  7. 辛民敎. 臨床本草學. 서울, 永林社, pp 128-132, 169, 221, 372-374, 400-406, 509-511, 1992
  8. 이경섭. 竹瀝湯, 加味竹瀝湯이 血壓 및 血糖에 미치는 影響. 慶熙大學校 大學院 博士學位論文, 1980
  9. 김영안, 변일. 중풍치료에 응용되는 竹瀝과 地龍의 효능에 대한 문헌적 고찰. 대전대학교 한의학연구소 논문집, 2(1):91- 104, 1993
  10. 정태호. 秋石 및 竹瀝이 白鼠의 血壓降下에 미치는 影響. 慶熙大學校 大學院 碩士學位論文, 1982
  11. 孫錫慶. 十宣穴 鍼刺와 竹瀝의 倂用이 白鼠의 血壓降下에 미치는 影響. 慶熙韓醫大 論文集 4卷, pp 27-38, 1981
  12. 최건기, 장경선, 오영준, 최찬헌. 생산공법차이에 따른 竹瀝의 성분 및 혈당강하효능 비교. 동의생리병리학회지 19(1):152- 158, 2005
  13. 장경선, 오영준, 최찬헌, 전용석. 생산공법 차이에 따른 竹瀝이 streptozotocin으로 유발된 당뇨생쥐에 미치는 영향. 동의생리병리학회지 16(6):1253-1259, 2002
  14. 장경선, 정동주, 최찬헌, 오영준. 생산 공법차이에 따른 竹瀝과 누에가루를 배합한 약물이 db/db mouse의 혈당강하에 미치는 영향. 동의생리병리학회지 17(5):1217-1223, 2003
  15. 윤대환, 이봄비, 심인섭, 나창수. 足三里에 施術된 竹瀝 藥鍼이 Intraluminal Filament 揷入術에 의하여 誘發된 白鼠의 虛血性 局所 腦損傷에 미치는 影響. 대한경락경혈학회지 22(2):25-41, 2005
  16. Longa, E.Z., Weinstein, P.R., Carlson, S., Cummins, R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 20(1):84-91, 1989 https://doi.org/10.1161/01.STR.20.1.84
  17. 진형석, 남상경, 이원철. 中風의 時代的 發展變化와 文獻的 考察. 경산대학교 제한동의학술원. 동서의학, 17(2):22-39, 1992
  18. 노현태, 김병탁. 中風의 誘發因子에 대한 文獻的 考察. 대전대학교 한의학연구소 논문집, 5(2):305-317, 1997
  19. 정현우, 오찬호, 은재순. 竹瀝 물분획이 생쥐 면역세포의 cytokine 분비에 미치는 영향. 동의병리학회지 13(1):79-83, 1999
  20. 김해진, 김선민, 오영준, 정기상, 장경선. 정제 방법에 따른 竹瀝의 물리.화학적 특성 연구(I). 동의생리병리학회지 15(3): 473-476, 2001
  21. 김인세, 박두진, 권재영, 김해규, 백승완, 신상욱. 백서의 중대뇌동맥 폐쇄에 의한 허혈성 뇌손상 모델에서 Dexamethasone과 MK-801이 뇌부종 형성에 미치는 영향. 대한마취학회지 37: 327-333, 1999
  22. Gill, R., Foster, A., Woodruff G. MK-801 is neuroprotective in gerbils when administered during the post-ischemic period. Neuroscience, 25: 847-855, 1988 https://doi.org/10.1016/0306-4522(88)90040-1
  23. Buchan, A.M., Pulsinelli, W.A. Hypothermia but not the N-methyl-D-aspartate receptor antagonist, MK-801, attenuates neuronal damage in gerbils subjected to transient global ischemia. J Neurosci. 11: 1049-1050, 1990
  24. Petito, C.K., Feldmann, E., Pulsinelli, W.A., Plum, F. Delayed hippocampal damage in humans following cardiorespiratory arrest. Neurology. 37(8):1281-1286, 1987 https://doi.org/10.1212/WNL.37.8.1281
  25. Hergruido, M.J., Carceller, F., Roda, J.M., Avendano, C. Hippocampal cell loss in transient global cerebral ischemia in rat. A critical assessment. Neuroscience. 93: 71-80, 1999 https://doi.org/10.1016/S0306-4522(99)00163-3
  26. Buchan, A., Pulsinelli, W.A. Hypothermia but not the N-methyl-D-aspartate antagonist, MK-801, attenuates neuronal damage in gerbils subjected to transient global ischemia. J Neurosci. 10(1):311-316, 1990 https://doi.org/10.1523/JNEUROSCI.10-01-00311.1990
  27. Foster, A.C., Gill, R., Woodruff, G.N. Neuroprotective effects of MK-801 in vivo: selectivity and evidence for delayed degeneration mediated by NMDA receptor activation. J Neurosci. 8(12):4745-4754, 1988 https://doi.org/10.1523/JNEUROSCI.08-12-04745.1988
  28. 이민섭, 안영준, 최기용, 강구, 강성식, 정일영, 이건재, 김근우. Nicotinamide의 투여가 일싲거인 국소허혈로 유발된 흰쥐의 뇌경색에 미치는 영향. The korean Journal of Pathology 40: 93-102, 2006
  29. Wyllie, A.H. Cell Death: a new classification separating apoptosis from necrosis. In: Bowen ID, Lockshin RA, eds, Cell Death in Biology and Pathology London: Chapman & Hall, pp 9-34, 1981
  30. Schwartzman, A.R., Cidlowski, A.J. Apoptosis The biochemistry and molecular biology of programmed cell death. Endocrine Reviews, 14(2):133-151, 1993
  31. Chong, M.J., Murray, M.R., Gosink, E.C., et al. Atm and Bax cooperate in ionizing radiation-induced apoptosis in the central nervous system. Proc Natl Acad Sci. 97: 889-894, 2000
  32. Oltvai, Z.N., Milliman, C.L., Korsmeyer, S.J. Bcl heterodimerizes in vivo with a comserved homolog, Bax, that accelerate programmed cell death. Cell 74: 609-619, 1993 https://doi.org/10.1016/0092-8674(93)90509-O
  33. 선웅. Bcl-2 family의 신경세포 사멸 조절과 신경계발달 Control of neuronal cell death by Bcl-2 family molecules. 한국생화학분자생물학회, 26(1):9-17, 2006
  34. Lee, M.S., Kang, G. A study fo apoptosis induced by microinjection of cytochrome c protein into mouse 3T3 fibroblast. The Korean journal of pathology 36: 1-6, 2002
  35. 김해규, 김평주, 백승완, 김인세, 정규섭. 흰쥐에서 뇌허혈시 해마의 Glutmate Receptor(mGluR5) 변화에 대한분자생물학적 연구. 대한중환자의학회지 15(2):75-81, 2000
  36. Arundine, M., Tymianski, M. Molecular mechanisms of glutamate-dependent neurodegeneration in ischemia and traumatic brain injury. Cell Mol Life Sci. 61: 657-668, 2004 https://doi.org/10.1007/s00018-003-3319-x
  37. Copani. A., Casabona, G., Bruno, V., Knopfel, T., Kuhn, R., Messina, A. mGluR5 receptor and apoptosis by trophic deprivation in primary clutures of cerebellar granule cells. Neuropharamcol 35(6):A9, 1996
  38. Maiese, K., Swiriduk, M., TenBroeke. M. Cellular mechanisms of protection by metabotropic glutmate receptors during anoxia and nitric oxide toxicity. J Neurochem 66: 2419-2428, 1996 https://doi.org/10.1046/j.1471-4159.1996.66062419.x
  39. Eduardo, E.B., et al. Medical neurosciences. fourth edition, LW&W, Minenesota, U.S.A. p 361, 1999
  40. Kang, W.S., Chun, M.H. Morphology and synaptic connectivity of cholinergic amacrine cells in the mouse retina. Korean J. Electron Microscopy 34(4):285-294, 2004