동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제21권5호
  • /
  • Pages.1163-1169
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  • 2007
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  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

소토사자환이 ob/ob mouse의 혈당, 고지혈증, Polyol Pathway 및 항산화작용에 미치는 영향

Effects of Sotosajahwan on Blood Glucose, Hyperlipidemia, Polyol Pathway and Antioxidative Mechanism in ob/ob Mouse

  • 박성호 (동국대학교 한의과대학 내과학교실) ;
  • 정지천 (동국대학교 한의과대학 내과학교실)
  • Park, Seong-Ho (Department of Internal Medicine, College of Oriental Medicine, Dongguk University) ;
  • Jeong, Ji-Cheon (Department of Internal Medicine, College of Oriental Medicine, Dongguk University)
  • 발행 : 2007.10.25
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초록

Effects of Sotosaja hwan on Blood Glucose, Hyperlipidemia, Polyol Pathway and Antioxidative Mechanism in ob/ob Mouse Diabetes is a disease in which the body does not produce or properly use insulin. Etiological studies of diabetes and its complications showed that oxidative stress might play a major role. Therefore, many efforts have been tried to regulate free oxygen radicals for treating diabetes and its complications. Sotosaja-hwan has been known to be effective for the antiaging and composed of four crude herbs. In male ob/ob mouse in severe obesity, hyperinsulinemia and hyperlipidemia, which are features of NIDDM, the hyperglycemic activites and mechanisms of Sotosaja-hwan were examined. Mice were grouped and treated for 5 weeks as follows. Both the lean (C57/BL6J black mice) and diabetic (ob/ob mice) control groups received standard chow. The experimental groups were fed with a diet of chow supplemented with 30 and 90 mg Sotosaja-hwan per 1 kg of body weight for 14 days. The effects of Sotosaja-hwan extract on the ob/ob mice were observed by measuring the serum levels of glucose, insulin, lipid components, and the kidney levels of superoxide anion radical $({\cdot}O_2)$, MDA+HAE, GSH/GSSG ratio, and also the enzyme activities involved in polyol pathway. Sotosaja-hwan lowered the levels of serum glucose and insulin in a dose dependent manner. Total cholesterol, triglyceride and free fatty acid levels were decreased, while the HDL-cholesterol level was increased, in Sotosaja-hwan treated groups. Renal aldose reductase and sorbitol dehydrogenase activities were increased in the ob/ob mice, whereas those were inhibited in the Sotosaja-hwan-administered groups. Sotosaja-hwan inhibited the generation of ${\cdot}O_2$ in the kidney. Finally, MDA+HAE levels was increased and GSH/GSSG ratio was decreased in the ob/ob mice, whereas those were improved in the Sotosaja-hwan-administered groups. Sotosaja-hwan showed the antidiabetic and anti hyperlipidemic activities by regulating the activities of polyol pathway enzymes, scavenging reactive oxygen species and reducing the MDA+HAE levels in the ob/ob mice.

키워드

참고문헌

  1. Kahn, C.R. The molecular mechanism of insulin action. Ann Rev Med. 34: 145-160, 1985
  2. 김응진 외. 당뇨병학. 서울, 고려의학, pp 15-43, 32-33, 149-169, 391-468, 475-487, 609-616, 1998
  3. 민헌기. 임상내분비학. 서울, 고려의학, pp 394-414, 1999
  4. Defronzo, R.A. Pathogenesis of type 2 diabetes. Metabolic and molecular implications for identifying diabetes genes. Diabetes Rev. 5: 177-269, 1997
  5. Reaven, G.M. Pathophysiology of insulin resistance in human disease. Physiol Rev. 75: 473-486, 1995 https://doi.org/10.1152/physrev.1995.75.3.473
  6. Polonsky, K.S. et al. Non-insulin dependent diabetes mellitus. A genetically programmed failure of the ${\beta}$ cell to compensate for insulin resistance. New Engl J Med. 334: 777-783, 1998 https://doi.org/10.1056/NEJM199603213341207
  7. Wolff, S.P. The potential role of oxidative stress in diabetes and its complications : Novel implication for theory and therapy in diabetic complications, Scientific and Clinical Aspects, Crabbe MJC, ed. NY: ChurchilLivingstone. pp 167-220, 1987
  8. Baynes, J.W. Role of oxidative stress in development of complications in diabetes. Diabetes. 40: 405-412, 1991 https://doi.org/10.2337/diabetes.40.4.405
  9. Hammers, H.D., Martin, S., Fedesrlin, K., Geisen, K. and Brownlee, M. Aminoguanidine treatment inhibit the development of experimental diabetic retinopathy. Proc Natl Acad Sci USA. 88: 11555-11558, 1991
  10. Pugliese, G., Tilton, G.T. and Williamson, J.R. Glucose-induced metabolic imbalance in the pathogenesis of diabetic vascular disease. Diabetes Metab Rev. 7: 35-59, 1991 https://doi.org/10.1002/dmr.5610070106
  11. Stevens, M.J., Dananberg, J., Feldman, E.L., Lattimer, S.A., Kamijo, M., Thomas, T.P., Shindo, H., Sima, A.A. and Greene, D.A. The linked roles of nitric oxide, aldose reductase and Na-K-ATPase in the slowing of nerve conduction in the streptozotocin diabetic rat. J Clin Invest. 94: 853-859, 1994 https://doi.org/10.1172/JCI117406
  12. 方藥中. 實用中醫內科學. 上海, 上海科學技術出版社, p 477, 1986
  13. 余永譜. 中醫治療內分泌代謝疾病. 浙江, 浙江科學技術出版社, p 239, 243, 1992
  14. 강석봉. 消渴의 傳變證과 당뇨병의 만성합병증에 대한 비교 고찰. 대한한의학회지 19(2):137-152, 1998
  15. 한기선. ob/ob mice에서 순기산의 항당뇨 활성 및 기전 연구. 경희대학교 대학원 석사학위논문, 2002
  16. 장경선, 정기상, 최찬헌, 오영준. 竹瀝과 누에가루 배합약물이 db/db mouse의 혈당 강하에 미치는 영향. 동의생리병리학회지 17(3):759-764, 2003
  17. 김형준, 윤철호, 정지천. 珍糖元의 고혈당 조절 작용 및 기전에 관한 연구. 대한한방내과학회지 25(2):277-287, 2004
  18. 이철웅, 정지천, 신현철. 고혈당 흰쥐에서 蠐螬의 혈당 조절과 항산화작용에 관한 연구. 대한한의학회지 27(1):91-103, 2006
  19. 陳師文 등 編. 太平惠民和劑局方. 臺北, 旋風出版社, 卷五, p 10, 1985
  20. 朱震亨. 丹溪心法. 北京, 北京市中國書店, p 221, 1986
  21. 王毅, 靳長金 朱君波 編著. 益壽效方 120, 北京, 中國醫藥科技出版社, pp 52-53, 1989
  22. Thomson, R.H. Colorimetric glucose oxidase method for blood glucose. Clin Chem Acta. 13: 133-135, 1966 https://doi.org/10.1016/0009-8981(66)90281-6
  23. Levinson, S.S. Use of an enzymatic method for cholesterol, designed for continuous flow instrumention, with discrete bichromatic and centrifugation analysis. Clin Chem. 23: 2335-2337, 1977
  24. Flether, M.J. A colorimetric method for estimation serum triglyceride. Clin Chem. 22: 393, 1968 https://doi.org/10.1016/0009-8981(68)90041-7
  25. 金井 泉 외. 臨床檢査法槪要. 동경, 금원, p 467, 1983
  26. Yamaoka, T., Nishimura, C., Yamashita, K., Itakura, M., Yamada, T., Fujimoto, J. and Kokai, Y. Acute onset of diabetic pathological changes in transgenic mice with human aldose reductase cDNA. Diabetologia. 38: 255-261, 1995 https://doi.org/10.1007/BF00400627
  27. Hollmann, S. In Hoppe-Seyler Thiefelder : Handbuch der physiol. und path.-chem. Vol. VIa. Berlin-Heidelberg-New York:Analyse, Springer p 704, 1964
  28. Cathcart, R., Schwiers, E., Ames, B.N. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay. Anal Biochem. 134: 111-116, 1983 https://doi.org/10.1016/0003-2697(83)90270-1
  29. Esterbauer, H., Schaur, R.J., and Zollner, H. Chemistry and Biochemistry of 4-Hydroxynonenal, Malondialdehyde and Related Aldehydes. Free Rad Biol Med. 11: 81-128, 1991 https://doi.org/10.1016/0891-5849(91)90192-6
  30. Gaitonide, M.K. A spectrophotometric method for the direct determination of cystein in the presense of other naturally occuring amino acid. Biochem J. 104: 627, 1967 https://doi.org/10.1042/bj1040627
  31. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. Protein measurement with folin phenol reagent. J Biol Chem. 193: 265-275, 1951
  32. Mordes, J.P. and Rossini, A.A. Animal models of diabetes mellitus. In Joslin's diabetes mellitus. pp 110-137, 1985
  33. Eleazar, S. Animal models of non-insulin-dependent diabetes. Diabetes metabolism reviews. 8(3):179-208, 1992 https://doi.org/10.1002/dmr.5610080302
  34. Vendemiale, G., Altomare, E., Grattagliano, I., Albano, O. Increased plasma levels of glutathione and malondialdehyde after avute ethanol ingestion in humans. J hepatol. 9: 359, 1989 https://doi.org/10.1016/0168-8278(89)90146-3