Experimental Study of Patholobi Caulis on the Transient Cerebral Ischemia in Rats

계혈등(鷄血藤) 추출물이 뇌허혈에 미치는 실험적 연구

  • 이상록 (동신대학교 한의과대학) ;
  • 최찬헌 (동신대학교 한의과대학) ;
  • 백진웅 (동신대학교 한의과대학) ;
  • 윤대환 (동신대학교 한의과대학) ;
  • 정상훈 (우석대학교 한의과대학) ;
  • 한웅 (우석대학교 한의과대학) ;
  • 정현우 (동신대학교 한의과대학) ;
  • 김계엽 (동신대학교 물리치료학과)
  • Published : 2007.10.25

Abstract

The study was designed to investigate the mechanism of Patholoobi Caulis freeze dried powder (PCF) on the regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats and cytokines production ($IL-1{\beta}$, $TNF-{\alpha}$, IL-10, $TGF-{\beta}$) in cerebral ischemic rats. The results in normal rats were as follows ; Increase of PCF-induced rCBF was significantly inhibited by pretreatment with methylene blue (10 ${\mu}g/kg$, I.p.), an inhibitor of guanylate cyclase, and was inhibited by indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase. Increase of PCF-induced MABP was decreased by pretreatment with methylene blue, but was increased by indomethacin. These results suggested that the mechanism of action PCF was mediated by cyclic 3',5'-guanosine monophosphate. The results in cerebral ischemic rats were as follows ; In cytokine production in serum from femoral arterial blood 1 hr after middle cerebral arterial occlusion, PCF (10 mg/kg. i.p.) significantly decreased $IL-1{\beta}$ and $TNF-{\alpha}$ production, and increased IL-10 production compared with control group. In cytokine production in serum from femoral arterial blood 1 hr 1 hr after reperfusion, PCF (10 mg/kg, i.p.) significantly decreased $IL-1{\beta}$ production, and incresed IL-10 production compared with control group. These results suggested that PCF was significantly and stably increased regional cerebral blood flow by inhibiting $IL-1{\beta}$ production, and by accelerating IL-10 production.

Keywords

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