Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of ${\alpha}-spinasterol$ Extracted from Phytolacca americanna on the Apoptosis of U937 cell line

상륙에서 추출한 ${\alpha}-spinasterol$의 백혈병세포주(U937) 자멸사 유도 효능

  • Yang, Jun-Seok (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Sang-Hun (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Kim, Ho (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Han, Ung (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Jin, Jae-Ho (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Jung, Il-Kook (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Dae-Keun (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Jeong, Seung-Il (Jeonju Biomaterials Institute) ;
  • Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Kwang-Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
  • 양준석 (우석대학교 한의과대학 병리학교실) ;
  • 정상훈 (우석대학교 한의과대학 병리학교실) ;
  • 김호 (우석대학교 한의과대학 병리학교실) ;
  • 한웅 (우석대학교 한의과대학 병리학교실) ;
  • 진재호 (우석대학교 한의과대학 병리학교실) ;
  • 정일국 (우석대학교 한의과대학 해부학교실) ;
  • 김대근 (우석대학교 한의과대학 해부학교실) ;
  • 정승일 (전주 생물소재 연구소) ;
  • 정한솔 (우석대학교 한의과대학 병리학교실) ;
  • 이광규 (우석대학교 한의과대학 병리학교실)
  • Published : 2007.10.25
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Abstract

To investigate the possible mechanism of ${\alpha}-spinasterol$ as a candidate of anti-cancer drug, I examined the effects of ${\alpha}-spinasterol$ on the apoptosis of U937 cells MTT assay, flow cytometric analysis, SDS-polyacrylamide gel electrophoresis, Western blot analysis, and RT-PCR were performed. ${\alpha}-spinasterol$ treatment reduced the cell viablilty of U937 cells in a dose-dependent manner, which was associated with the induction of apoptotic cell death. ${\alpha}-spinasterol$ treatment also reduced the levels of Bcl-xL anti-apoptotic protein expression and increased the levels of caspase-3, p53, pro-apoptotic protein, in U937 cells. After treatment the level of Bcl-xL, anti-apoptotic gene expression was decreased and the level of ICE pro-apoptotic gene expression was increased. These findings suggest that ${\alpha}-spinasterol$ induced the apoptotic cell death via regulation of several growth regulatory gene products. The abbreviations used are: FBS, fetal bovine serum; PBS, phosphate buffered saline; PI, propidium iodide; OD, optical density; DiOC6, 3,3-dihexyloxa carbcyanine iodide; MTT, 3 [4-5-dimethylthiazol-2-yl] -2-diphenyltetrazolium bromide.

Keywords

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