Journal of Crop Science and Biotechnology
- Volume 10 Issue 1
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- Pages.13-18
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- 2007
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- 1975-9479(pISSN)
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- 2005-8276(eISSN)
Use of Near-Infrared Spectroscopy for Estimating Fatty Acid Composition in Intact Seeds of Rapeseed
- Kim, Kwan-Su (Department of Medicinal Plant Resources, Mokpo National University) ;
- Park, Si-Hyung (Department of Medicinal Plant Resources, Mokpo National University) ;
- Choung, Myoung-Gun (Department of Pharmacognosy Material Development, Kangwon National University) ;
- Jang, Young-Seok (Mokpo Experiment Station, National Institute of Crop Science, Rural Development Administration)
- Published : 2007.03.31
Abstract
Near-infrared spectroscopy(NIRS) was used as a rapid and nondestructive method to determine the fatty acid composition in intact seed samples of rapeseed(Brassica napus L.). A total of 349 samples(about 2 g of intact seeds) were scanned in the reflectance mode of a scanning monochromator, and the reference values for fatty acid composition were measured by gas-liquid chromatography. Calibration equations for individual fatty acids were developed using the regression method of modified partial least-squares with internal cross validation(n=249). The equations had low SECV(standard errors of cross-validation), and high