Reproductive and Developmental Biology

  • 제31권1호
  • /
  • Pages.29-33
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  • 2007
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

소 체외수정란 및 체세포 복제란의 초자화 동결 후 생존성

Survivability of In Vitro Fertilized and Somatic Cell Nuclear Transfer Bovine Embryos Following Vitrification

  • 권대진 (강원대학교 동물생명과학대학) ;
  • 박주희 (강원대학교 수의학부대학) ;
  • 박춘근 (강원대학교 동물생명과학대학) ;
  • 양부근 (강원대학교 동물생명과학대학) ;
  • 정희태 (강원대학교 수의학부대학)
  • Kwon, Dae-Jin (College of Animal Life Science Kangwon National University) ;
  • Park, Joo-Hee (School of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Science Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Science Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
  • 발행 : 2007.03.31
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초록

본 연구는 소 체외수정란과 체세포 복제란의 초자화 동결 및 응해 후 생존능을 검토하였다. 배반포로 발육된 체외수정란 및 체세포 복제란을 초자화 동결법에 의해 동결하였다가 융해하여 생존율 및 배양 후 부화율을 검사하였다. 체외수정란 배반포를 초자화 동결 응해한 결과, 확장배반포가 배반포기 난자에 비하여 생존율(82.1%, 96/117)과 부화율(64.1%, 75/117)에서 모두 유의적으로 높았다(p<0.05). 핵이식 배반포 복제란을 초자화 동결 응해한 경우도 체외수정란과 비슷한 경향을 보여 확장 및 부화배의 생존율과 부화율이 각각 81.1%(30/37)와 78.3%(29/37)로, 배반포(각각 71.8 및 53.8%)에 비하여 다소 높게 나타났다. 본 연구의 결과는 초자화 동결 방법에 의해서 소 체외수정란과 체세포 복제란을 성공적으로 동결할 수 있으며, 특히 후기 배반포기 단계에서 초자화 동결 시 높은 생존율과 부화배 형성율을 얻을 수 있음을 보여준다.

This study was conducted to examine the development of in vitro fertilized (IVF) and nuclear transfer (NT) embryos following vitrification IVF and NT embryos developed to the blastocyst stage were equilibrated by 3 steps, vitrified and thawed, and their survival and hatching rates were examined. In IVF embryos, higher survival (82.1%, 96/117) and hatching rates (64.1%, 75/117) were obtained respectively after thawing and culture in expanded blastocysts compared to blastocysts (p<0.05). High survival and hatching rates were also obtained by vitrification of NT blastocysts, especially in expanded and hatching blastocysts (81.1 and 78.3%, respectively). The result of this study shows that IVF and NT blastocysts, especially late stage blastocysts, are successfully cryopreserved by vitrification.

키워드

참고문헌

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