Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Alteration of DNA Methylation in Oct-4 Gene in Mouse Preimplantation Embryos by the Interference RNA

  • Kim, Jong-Mu (National Research Laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University) ;
  • Ko, Yeoung-Gyu (Animal Biotechnology Division, National Livestock Research institute) ;
  • Seong, Hwan-Hoo (Animal Biotechnology Division, National Livestock Research institute) ;
  • Chung, Hak-Jae (Animal Biotechnology Division, National Livestock Research institute) ;
  • Chang, Won-Kyong (Animal Biotechnology Division, National Livestock Research institute) ;
  • Kim, Nam-Hyung (National Research Laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University)
  • Published : 2007.03.31
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Abstract

During early embryo development, Oct-4 is an important transcription factor for the early differentiation the present study was first examined methylation status in distal enhancer and promoter region of Oct-4 during mouse pre-implantation embryo development. In oocyte and sperm, high methylation was observed in both distal and proximal of promoter in Oct-4. Following fertilization relatively high methylation level remained until 8-cell stage embryos, but decreased at the morula and blastocyst stage. Specific gene knock down of Oct-4 by siRNA injection into zygote induced higher methylation rates of both distal and proximal region of promoter of Oct-4. These results suggest a functional link between the DNA methylation status of distal and promoter resign in the Oct-4 gene and the gene sequence-specific transcriptional silencing by exogenous siRNA injection during mouse preimplantation embryos.

Keywords

References

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