KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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SSR Marker Linked to f Locus in Soybean

  • Nam, Ki-Chul (Division of Applied Life Science, Agronomy major, Research Institute of Life Sci., Gyeonsang National University) ;
  • Kim, Myung-Sik (Division of Applied Life Science, Agronomy major, Research Institute of Life Sci., Gyeonsang National University) ;
  • Jeong, Woo-Hyeun (Division of Applied Life Science, Agronomy major, Research Institute of Life Sci., Gyeonsang National University) ;
  • Kim, Seok-Hyeon (Division of Applied Life Science, Agronomy major, Research Institute of Life Sci., Gyeonsang National University) ;
  • Chung, Jong-Il (Division of Applied Life Science, Agronomy major, Research Institute of Life Sci., Gyeonsang National University)
  • Published : 2007.03.31
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Abstract

Soybean has a morphological type with a broadened and flattened stem. Fasciation has been suggested as a new gene for soybean research. SSR marker linked to the $\Large f$ locus that controls fasciation phenotype has not identified within 10 cM. A mapping population consisting of 94 $F_2$ progenies was derived from a cross between wild type Clark (FF) and fasciation mutant C32 (${\Large f}{\Large f}$). The phenotype of $F_2$ individual plants was recorded at R2 and R3 growth stage from field. One-thousand 10-mer oligonucleotide RAPD primers and 29 SSR primers selected from the D1b+W of the soybean molecular linkage map were used. A genetic map was constructed from the segregating 35 RAPD, four SSR markers and one phenotypic(wild type/fasciation) marker. The segregation ratios of 3 : 1 observed in the $F_2$ population and the Chi-square values strongly suggest that the fasciation trait is controlled by a single recessive gene. Satt537 marker was linked to $\Large f$ locus at a distance of 9.6 cM. Assignment of the $\Large f$ locus to linkage group D1b+W and identification of markers can be used as an initial step for fine mapping of the $\Large f$ gene.

Keywords

References

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