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Control of Powdery Mildew of Pepper Using Culture Solutions of Chitinolytic Bacteria, Chromobacterium sp. and Lysobacter enzymogenes

키틴분해세균 Chrobacterium sp.와 Lysobacter enzymogenes의 배양액을 이용한 고추 흰가루병의 방제

  • Seo, Chong-Chan (Department of Agricultural Biology, Sunchon National University) ;
  • Jung, Hyun-Chae (Department of Forest Resources, Sunchon National University) ;
  • Park, Seur-Kee (Department of Agricultural Biology, Sunchon National University)
  • Published : 2007.04.01

Abstract

Powdery mildew of pepper is one of the most devastating diseases which is occurring all the year under greenhouse condition. In this study, control efficacy against powdery mildew was evaluated by mixed culture solutions of two chitinolytic bacteria, Lysobacter enzymogenenes strain C-3 and Chrornobacterium sp. strain C-61, cultivated in the chitin-supplemented medium. In all experiments, white powder on the reverse side of pepper leaves perfectly disappeared 3 days after application of mixed culture solutions. However, periods required for formation of new white powder on the same sites after application (control-lasting period) were largely differed according to environmental conditions. In particular, the control-lasting period was much longer when sprayed on 6 PM than 9 AM and especially, on rainy days than sunny days. This indicates that control efficacy of culture solution may be largely affected by environmental conditions after application. The undiluted culture solution resulted in a perfect control with control value more than 95% by application of 5-day-intervals under severely diseased field and 7-day-intervals under disease-started field. A ten-fold diluted product also showed control value more than 81% by application of the same method. These results suggest that this culture solution can be practically used to control powdery mildew disease in pepper plants.

비닐하우스에서 고추 흰가루병은 1년 내내 발생하는 가장 문제시되는 병해중의 하나이다. 본 연구에서는 키틴 함유배지에서 자란 L. enzymogenes strain C-3와 Chromobacterium sp. strain C-61의 혼합배양액에 의한 흰가루병 방제효과를 평가하였다. 모든 실험에서 고추 잎 뒷면에 분포하는 흰가루는 혼합배양액 살포 3일 후 완전 사라졌다. 그러나 동일 부위에서 새로운 흰가루가 나타나기까지의 기간(방제지속기간)은 살포후의 환경조건에 따라 크게 달랐다. 특히, 오전 9시보다 오후 6시에 살포되었을 경우, 그리고 살포 다음날 비가 왔을 경우에는 그 방제지속기간이 훨씬 더 길었다. 이것은 살포 후의 환경 조건이 병 방제효과에 크게 영향을 미칠 것이라는 것을 암시한다. 배양 원액은 병 발생이 심한 포장에서 5일 간격, 병 발생이 시작되는 포장에서 7일 간격 살포함으로서 95% 이상의 완전한 방제효과를 보여 주었다. 10배 희석 액도 동일 방법으로 살포하였을 경우 81% 이상의 방제 효과를 나타냈다. 따라서 본 배양액은 고추 흰가루병 방제에 실제 이용 가능할 것으로 판단된다.

Keywords

References

  1. Blazquez, C. H. 1976. A powdery mildew of chilli caused by Oidiopsis sp. Phytopathology 66: 1155-1157 https://doi.org/10.1094/Phyto-66-1155
  2. 차재순, 기운계, 조백효, 김기청. 1980. 고추에 발생하는 흰가루병. 한식보호지 19: 241-243
  3. Correll, J. C., Gordon, T. R. and Elliott, V. J. 1987. Host range, specificity, and biometrical measurements of Leveillula taurica in California. Plant Dis. 71: 248-251 https://doi.org/10.1094/PD-71-0248
  4. Dunne, C., Crowley, J. J., Monne-Loccoz, Y., Dowling, D. N., de Bruijn, F. J. and O'Gara, F. 1997. Biological control of Pythium ultimum by Stenotrophomonas maltophilia W81 is mediated by an extracellular proteolytic activity. Microbiology 143: 3921-3931 https://doi.org/10.1099/00221287-143-12-3921
  5. Elad, Y., Malathrakis, N. E. and Dik, A. J. 1996. Biological control of Botrytis-incited diseases and powdery mildews in greenhouse crops(Review). Crop Prot. 15: 229-240 https://doi.org/10.1016/0261-2194(95)00129-8
  6. Fallik, E., Ziv, O., Grinberg, S., Alkalai, S. and Klein, J. D. 1997. Bicarbonate solutions control powdery mildew (Leveillula taurica) on sweet red pepper and reduce the development of postharvest fruit rotting. Phytoparasitica 25: 41-43 https://doi.org/10.1007/BF02981478
  7. Her, M. E., Kim, E. H., Seo, J. C. and Park, S. K. 2005. Improvement of biocontrol of cucumber powdery mildew by culture solution of chitinolytic bacteria (ABSTRACT). Plant Pathol. J. 21: 437
  8. Jakobi, M. and Winkelmann, G. 1996. Maltophilin: a new antifungal compound produced by Stenotrophomonas maltophilia R3089. J. Antibiot. 49: 1101-1104 https://doi.org/10.7164/antibiotics.49.1101
  9. 이혹희, 황화숙, 김주영, 한정혜, 유영신, 김병수. 2001. 고추 흰가루병에 대한 저항성재료 탐색. 원예과학기술지 19: 7-11
  10. Park, S. K., Lee, M. C. and Harman, G. E. 2005. The biocontrol activity of Chromobacterium sp. strain C-61 against Rhizoctonia solani depends on the productive ability of chitinase. Plant Pathol. J. 21: 275-282 https://doi.org/10.5423/PPJ.2005.21.3.275
  11. Reuveni, R., Peri, M. and Rotem, J. 1974. The effect of Leveillula taurica on leaf abscission in peppers. Phytopathol. Z. 80: 79-84 https://doi.org/10.1111/j.1439-0434.1974.tb02732.x
  12. Reuveni, R., Dor, G. and Reuveni, M. 1998. Local and systemic control of powdery mildew(Leveillula taurica) on pepper plants by foliar spray of mono-potassium phosphate. Crop Prot. 17: 703-709 https://doi.org/10.1016/S0261-2194(98)00077-5
  13. 신현동. 2000. 한국식물병원체 1. 흰가루병균과. 농업과학기술원. 320 pp
  14. Sullivan, R. F., Holtman, M. A., Zylstra, G. J., White, J. F. and Kobayashi, D. Y. 2003. Taxonomic positioning of two biological control agents for plant diseases as Lysobacter enzymogenes based on phylogenetic analysis of 16S rDNA, fatty acid composition and phenotypic characteristics. J. Appl. Microbiol. 94: 1079-1086 https://doi.org/10.1046/j.1365-2672.2003.01932.x
  15. Zhang, Z. and Yuen, G. Y. 2000a Effects of culture fluids and preinduction of chitinase production on biocontrol of bipolaris leaf spot by Stenotrophomonas maltophilia C3. Biol. Control 18: 277-286 https://doi.org/10.1006/bcon.2000.0834
  16. Zhang, Z. and Yuen, G. Y. 2000b. The role of chitinase production by Stenotrophomonas maltophilia strain C3 in biological control of Bipolaris sorokiniana. Phytopathology 90: 384-389 https://doi.org/10.1094/PHYTO.2000.90.4.384
  17. Zhang, Z., Yuen, G. Y., Sarath, G. and Penheiter, A. R. 2001. Chitinases from the plant disease biocontrol agent, Stenotrophomonas maltophilia C3. Phytopathology 91: 204-211 https://doi.org/10.1094/PHYTO.2001.91.2.204

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  2. Control Efficacy of Mixed Application of Microbial and Chemical fungicides against Powdery mildew of red-pepper vol.18, pp.4, 2014, https://doi.org/10.7585/kjps.2014.18.4.409