Journal of Biosystems Engineering

  • 제32권1호
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  • Pages.50-56
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  • 2007
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  • 1738-1266(pISSN)
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  • 2234-1862(eISSN)
한국농업기계학회 (Korean Society for Agricultural Machinery)
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무균돼지뼈를 이용한 복합 골지지체의 제조와 생체적합성 평가

Preparation and Biocompatibility of Composite Bone Scaffolds Using Gnotobiotic Pig Bones

  • Im, Ae-Lee (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Chung, Jong-Hoon (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Lim, Ki-Taek (Dept. of Biosystems & Biomaterials Science and Engineering, Seoul National University) ;
  • Choung, Pill-Hoon (Tooth Bioengineering National Research Lab., Dept. of Oral and Maxillofacial Surgery, College of Dentistry) ;
  • Hong, Ji-Hyang (Research Institute for Agriculture and Life Sciences, Seoul National University)
  • 발행 : 2007.02.25
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초록

Highly porous composite bioceramic bone scaffolds were developed using sintered gnotobiotic pig bones. These scaffolds consisted of poly-D,L-lactic acid (P(D,L)LA) and bioceramic materials of pig bone powder. The bone scaffolds were able to promote biocompatibility and possess interconnected pores that would support cell adhesion and proliferation adequately. The composite scaffolds were tested with dental pulp stem cells for cytotoxicity test. Cells seeded on the composite scaffolds were readily attached, well proliferated, as confirmed by cytotoxicity test, and cell adhesion assessment. The composite bone scaffold had no toxicity in cytotoxicity test on the extract of 0.013 g scaffold to 2 ml culture medium. The cells on the composite bone scaffold proliferated better than cells on the P(D,L)LA scaffolds.

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참고문헌

  1. Boer, H. H. 1988. The history of bone grafts. Clin. Orthop. Relat. Res. 226:292-298
  2. Bonfiglio, M and W. S. Jeter. 1972. Immunological responses to bone. Clin. Orthop. Relat. Res. 87:19-27
  3. Chung, J. H., W. Lee, P. H. Choung and J. E. Davies. 2004. A Novel Bioresorbable Bone Cement Using Tooth Apatite, Chitosan, and Cyanoacrylate for Bone Tissue Engineering. Journal of Biosystems Engineering 29(4):347-456. (In Korean) https://doi.org/10.5307/JBE.2004.29.4.347
  4. Freed, L. E., G. Vunjak-Novakovic, R. J. Biron, D. B. Eagles, D. C. Lesnoy, S. K. Barlow and R. Langer. 1994. Biodegradable polymer scaffolds for tissue engineering. Biotechnology 12:689-693 https://doi.org/10.1038/nbt0794-689
  5. Hou, Qingpu., Dirk. W. Grijpma and Jan. Feijen. 2003. Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique. Biomaterials 24:1937-1947 https://doi.org/10.1016/S0142-9612(02)00562-8
  6. Hutmacher, D. W. 2000. Scaffolds in tissue engineering bone and cartilage. Biomaterials 21:2529-2533 https://doi.org/10.1016/S0142-9612(00)00121-6
  7. Ishaug, S. L., G. M. Crane, M. J. Miller, A. W. Yasko, M. J. Yaszemski and A. G. Mikos. 1997. Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds. Journal of Biomedical Materials Research 36:17-28 https://doi.org/10.1002/(SICI)1097-4636(199707)36:1<17::AID-JBM3>3.0.CO;2-O
  8. Kim, E. S and J. H. Chung. 2004. Fabrication and Biomechanical characteristics of Composite Ceramic Bone Scaffolds for Bone Tissue Engineering. Journal of Biosystems Engineering 29(5):457-466. (In Korean) https://doi.org/10.5307/JBE.2004.29.5.457
  9. Krishna, S. R.. C. K. Chaitanya, S. K. Seshadri and T. S. Kumar. 2002. Fluorinated hydroxyapatite by hydrolysis under microwave irradiation. Trends Biomater. Artif. Organs. 16:15-17
  10. Lee, C. K and J. S. Choi. 1997. The Properties of Natural Hydroxyapatite Isolated kom Tuna Bone. J. Korean Fish. Soc. 30(4):652-659. (In Korean)
  11. Lin, F. H., C. J. Liao, K. S. Chen, J. S. Sun. 1999. Preparation of a biphasic porous bioceramic by heating bovine cancellous bone with $Na_4P_2O_710H_2O$ addition. Biomaterials 20:475-484 https://doi.org/10.1016/S0142-9612(98)00193-8
  12. Martin, R. B. 2000. Biomaterials. The Engineering Handbook CRC Press
  13. Tadic, D and M. Epple. 2004. A thorough physicochemical characterization of 14 calcium phosphate-based bone substitution materials in comparison to natural bone. Biomaterials 25:987-994 https://doi.org/10.1016/S0142-9612(03)00621-5
  14. Tancred, D. C., B. A. O. McCormack and A. J. Carr. 1998. A quantitative study of the sintering and mechanical properties of hydroxyapatite/phosphate glass composites. Biomaterials 19:1735-1743 https://doi.org/10.1016/S0142-9612(98)00082-9
  15. SAS User's Guide. 1990. Statistical Analysis System Institute Inc., Cary, NC, USA
  16. Vacanti, C. A,, W. Kim, J. Upton, M. P. Vacanti, D. Mooney and B. Schloo. 1993. Tissue-engineered growth of bone and cartilage. Transplant Proc. 25:1019-1021
  17. Yoo, James J and I. W. Lee. 1998. Tissue Engineering. Seoul, Korea Medical Publishing Company. (In Korean)

피인용 문헌

  1. Calcium phosphate bioceramics fabricated from extracted human teeth for tooth tissue engineering vol.99B, pp.2, 2011, https://doi.org/10.1002/jbm.b.31912
  2. Preliminary evaluation of bone graft substitute produced by bone of duck beak vol.121, 2014, https://doi.org/10.1016/j.matlet.2014.01.141