Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Hot Water Extraction of Crude Chondroitin Sulfate from Shark Cartilage Treated with Ozone Water

오존수로 세척한 상어연골로부터 Crude Chondroitin Sulfate의 열수추출

  • Jung, Yoo-Kyung (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Shin, Kyung-Ok (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Shin, Jong-Wook (Department of Food Science and Technology, Catholic University of Daegu) ;
  • No, Hong-Kyoon (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Kim, Soon-Dong (Department of Food Science and Technology, Catholic University of Daegu)
  • 정유경 (대구가톨릭대학교 식품외식산업학부) ;
  • 신경옥 (대구가톨릭대학교 식품외식산업학부) ;
  • 신종욱 (대구가톨릭대학교 식품외식산업학부) ;
  • 노홍균 (대구가톨릭대학교 식품외식산업학부) ;
  • 김순동 (대구가톨릭대학교 식품외식산업학부)
  • Published : 2007.09.30
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Abstract

This study was conducted to investigate the effect of ozone treatment on removal of brown color and ammonia-like odor produced from shark cartilage (SC) and to establish the optimal extraction conditions of chondroitin sulfate (CS) by NaOH and hot water. Treatment of SC with 0.3 ppm ozone water resulted in higher color $L^*$ value but lower $a^*$ and $b^*$ values compared with those of non-treated SC, without affecting its proximate compositions (moisture, protein, lipid, carbohydrate, and ash). Ozone treatment also increased the overall acceptability of SC by reducing ammonia-like odor. The optimum concentration and time for extraction of CS from SC using NaOH at $100^{\circ}C$ were 1 N and 120 min, respectively, with 10.02% of extraction yield. The total yield (10.01%) of CS obtained by 7 times repeated extraction with hot water at $100^{\circ}C$ was comparable to that of the 1 N NaOH extraction for 120 min at $100^{\circ}C$.

상어연골에서 발생하는 갈변과 냄새를 제거하기 위한 오존수 세척효과와 crude chondroitin sulfate의 추출특성을 조사하기 위하여 알칼리 및 열수추출 효과를 조사하였다. SC 분말을 용존 오존농도 0.3 ppm의 오존수로 세척한 결과 일반성분의 함량에는 영향을 미치지 않으면서 $L^*$값은 증가하고 $a^*$, $b^*$값은 감소되어 탈색효과가 뚜렷하였다. 또한 ammonia 유사취가 크게 감소되었으며 종합적 기호도가 크게 향상되었다. NaOH에 의한 CS의 최적 추출농도는 1 N이었으며 최적 추출시간은 $100^{\circ}C$에서 120분이었고 이때의 수율은 10.02%이었다. $100^{\circ}C$의 열수로 7회 반복추출 할 경우 1 N NaOH 추출수율과 대등하였다.

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