Journal of Oral Medicine and Pain

  • 제32권2호
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  • Pages.129-135
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  • 2007
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  • 2288-9272(pISSN)
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  • 2383-8493(eISSN)
대한안면통증구강내과학회 (Korean Academy of Orofacial Pain and Oral Medicine)
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Phytoecdysteroid가 조골세포와 파골세포의 성장과 활성에 미치는 영향

Effects of Phytoecdysteroid on the Proliferation and Activity of Bone Cells

  • 고선일 (단국대학교 치과대학 구강생화학교실)
  • Ko, Seon-Yle (Department of Oral Biochemistry, School of Dentistry, Dankook University)
  • 발행 : 2007.06.30
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초록

Ecdysteroid는 곤충의 탈피호르몬으로 알려져 있으며, phytoecdysteroid는 식물의 ecdysteroid로 포유동물에 여러 유용한 효과를 가진다고 알려져 있다. 본 연구는 식물의 phytoecdysteroids가 골대사에서 미치는 영향을 알아보기 위하여 세포수준에서 관찰하였다. 즉 조골세포에 미치는 영향을 알아보기 위하여 세포증식율, 염기성인산분해효소 활성, gelatinase 활성의 변화를 관찰하였고, 파골세포에 미치는 영향을 알아보기 위하여 tartrate-저항성 인산분해효소 양성인 다핵세포의 형성을 측정함으로써 관찰하였다. Phytoecdysteroid 처리에 의해 조골세포의 ALP 활성과, gelatinase의 활성이 증가되었다. 또한 phytoecdysteroid는 macrophage-colony stimulating factor (M-CSF)와 receptor activator of NF-kB ligand (RANKL)에 의해 유도된 파골세포의 생성을 감소시켰다. 이상의 결과 phytoecdysteroid는 조골세포와 파골세포의 활성 및 생성을 변화 시킴으로써 골수의 미세환경에서 세포내 조절작용에 관여하리라 여겨진다.

Ecdysteroids are known as insect molting hormone. At the same time, ecdysteroids and plant ecdysteroids (phytoecdysteorids) reveal beneficial effects on mammal. The present study was undertaken to determine the possible cellular mechanism of action of phytoecdysteroids in bone metabolism. The effects on the osteoblasts were determined by measuring cell proliferation, alkaline phosphatase (ALP) activity, and gelatinase activity. The effects on the osteoclasts were investigated by measuring tartrate-resistant acid phosphatase (TRAP)(+) multinucleated cells (MNCs) formation after culturing osteoclast precursors. Phytoecdysteroid treatment showed a increase in ALP activity of osteoblasts. Phytoecdysteroid increased the activity of gelatinase. In addition, phytoecdysteroid decreased the osteoclast generation induced by macrophage-colony stimulating factor (M-CSF) and receptor activator of NF-kB ligand (RANKL) in (M-CSF)-dependent bone marrow macrophage (MDBM) cell cultures. Taken these results, phytoecdysteroid may be a regulatory protein within the bone marrow microenvironment.

키워드

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