International Journal of Industrial Entomology and Biomaterials

  • 제14권1호
  • /
  • Pages.33-36
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  • 2007
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
권호 표지

Expression and Synergistic Effect of Bacillus thuringiensis CrylAc in Lepidopteran Toxic Strain to Plutella xylostella

  • Kang, Joong-Nam (Department of Agricultural Biotechnology, Seoul National University) ;
  • Roh, Jong-Yul (Department of Agricultural Biotechnology, Seoul National University) ;
  • Shin, Sang-Chul (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Ko, Sang-Hyun (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Chung, Yeong-Jin (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Kim, Yang-Su (Department of Agricultural Biotechnology, Seoul National University) ;
  • Wang, Yong (Department of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Hee-Kyu (Department of Agricultural Biotechnology, Seoul National University) ;
  • Li, Ming-Shun (Department of Agricultural Biotechnology, Seoul National University) ;
  • Choi, Jae-Young (Department of Agricultural Biotechnology, Seoul National University) ;
  • Je, Yeon-Ho (Department of Agricultural Biotechnology, Seoul National University)
  • 발행 : 2007.03.31
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초록

To improve insecticidal activity of B. thuringiensis 2385-1 (Bt 2385-1), a recombinant plasmid, pHT1K-1Ac, was introduced into lepidopteran toxic Bt 2385-1 by electroporation. The presence of the recombinant plasmid in Bt 2385-1 after electroporation was confirmed by PCR. Bt 2385-1 transformant was named as Bt pHT1K-1Ac/2385-1 (1K-1Ac/2385-1). The 1K-1Ac/2385-1 transformant produced bipyramidal-shaped parasporal inclusion as like the wild-type strain, Bt 2385-1, and showed an 130 kDa band of Cry1Ac protein. The insecticidal activity of 1K-lAc/2385-1 against S. exigua was similar to that of Bt 2385-1 but the $LC_{50}$ value of transformant against P. xylostella was 1.8 times lower. Through these bioassay results, it was confirmed that toxicity of Bt 2385-1 transformant showed synergistic effect by introducing Cry1Ac. These results suggested that the multiple expressions of Cry proteins in a promising Bt strain may interact synergistically in insect midgut, resulting in increase of toxicity and expansion of host spectrum.

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