Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Effects of Sun Ginseng on the Crypt Cell Survival in Mice Irradiated with High-energy X-ray

선삼 투여가 고에너지 X-선을 조사한 마우스에서 소장움 재생에 미치는 효과

  • Sin, Jung-Sub (Department of Public Health, Keimyung University) ;
  • Park, Jeong-Hill (College of Pharmacy, Seoul National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Hun-Jeong (Department of Radiation Oncology, Kyongju Hospital, Dongguk University) ;
  • Kim, Young-Chul (Department of Public Health, Keimyung University)
  • 신정섭 (계명대학교 공중보건학과) ;
  • 박정일 (서울대학교 약학대학) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 김현정 (동국대학교 경주병원 종양학과) ;
  • 김영철 (계명대학교 공중보건학과)
  • Published : 2007.03.31
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Abstract

Six week-old ICR mite which were divided into four groups including NC, RC, RR and RS were injected with sun ginseng (RS), red ginseng (RR) and saline (RC) intraperitonedlly as an amount of 60 mg/g body weight at 1 hour, 12 hours and 36 hours before the irradiation of high-energy X-ray and the mire were sacrificed at three and a half days after the irradiation. The RS group were significant increase in the weight of spleen (p<0.01) and the numbers of jejunal crypt cells (P<0.01), WBC (p<0.05), lymphocytes (p<0.05) and neutrophils (p<0.05) in comparison with the RC group. The RR group were significant increase in the numbers of jejunal crypt cells (p<0.001), WBC (p<0.05) and neutrophils (p<0.05) in comparison with the RC group. The RS group exhibited a more increase in the weights of spleen and thymus and the numbers of jejunal crypt cells and all items of hematological examination than the RR group. The values of ALT (alanine transaminase) and AST (aspartate transaminase) were significantly elevated (p<0.05) by radiation and they were significantly decreased (p<0.05) in the RS group to the values of the NC group. Taken together the above results, sun ginseng demonstrated a jejunal crypt survival effect, the protective effects on hepatocytes and immune and hematopoietic cells in mice irradiated with high-energy X-ray, and those radiation protective effects were a little higher in comparison with red ginseng.

Keywords

References

  1. 김동윤, 장재철. 홍삼 분획물이 감마선을 조사한 생쥐 간에서 항산화물질과 지질과산화에 미치는 방사선 보호 효과, 고려인삼학회지 1998; 22(1): 1-10
  2. 김성호, 이해준, 김세라, 이종환, 조성기, 나승렬, 손창호, 신동호. 방사선 피폭 마우스에서 홍삼, 백삼 및 diethyldithiocarbamate의 효과, 고려인삼학회지 2001; 25(2): 68-73
  3. 박정일. 약효가 강화된 새로운 가공인삼-선삼, 식품산업과 영양 2004; 9(1): 23-27
  4. 박혜란, 김성호, 이성태, 변명우, 조성기. 면역조혈계 및 재생조직의 방사선 손상에 대한생약 복합물(HIM-1)의 방호 효과, 한국식품영양과학회지 2005; 34(5): 605-612
  5. 안기정, 박성광, 조흥래, 강기문, 정덕화, 강진순, 채규영. 비타민 C의 방사선에 의한 독성 완화 효과, 대한방사선종양학회지 2004; 22(4): 280-287
  6. Bannasch P, Mayer D and Hacker HJ. Hepatocellular glycogenosis and hepatocarcinogenesis, Biochim Biophys Acta 1980; 605(2): 217-245
  7. Gough GR, Boggs SS and Schwartz GN. Changes in thymus size observed radiographically during the course of spontaneous lymphoma in the AKR/J mouse, Lab Anim Sci 1977; 27(5 Pt 1): 627-634
  8. Halliwell B and Gutterige JM. Free radical in biology and medicine, Oxford University Press. New York, 3rd ed, 1999; 604-607
  9. Hendry JH, Roberts SA and Potten CS. The clonogen content of murine intestinal crypts dependence on radiation dose used in its determination, Rad Res 1992; 132(1): 115-119 https://doi.org/10.2307/3578342
  10. Hsu HY, Lian SL and Lin CC. Radioprotective effect of Ganoderma lucidum (Leyss. ex. Fr.) Karst after X-ray irradiation in mice, Am J Chin Med 1990; 18(1-2): 61-69 https://doi.org/10.1142/S0192415X90000095
  11. IAEA. Manual on Early Medical Treatment of possible radiation Injury, Safety series IAEA Vienna 1978, Report No. 47, 1978; 74
  12. Ijiri K and Potten CS. Further studies on the response of intestinal crypt cells of different hierarchical status to eighteen different cytotoxic agents, Br J Cancer 1987; 55(2): 113-123 https://doi.org/10.1038/bjc.1987.25
  13. Kim WY, Kim JM, Han SM, Lee SK, Kim ND, Park MK and Park JH. Steaming of jinseng at hight temperature enhances biological activity, J Nat Prod 2000; 63(1): 702-704
  14. Lee HJ, Kim SR, Kim JC, Kang CM, Lee YS, Jo SK, Kim TH, Jang JS, Nah SY and Kim SH. In vivo radioprotective effect of Panax ginseng C.A. Meyer and identification of active ginsenosides, Phytother Res 2006; 20(5): 392-395 https://doi.org/10.1002/ptr.1867
  15. Li NQ. Clinical and experimental study on shen-qi injection with chemotherapy in the treatment of malignant tumor of digestive tract, Zhongguo Zhong Xi Yi Jie He Za Zhi 1992; 12(10): 588-592
  16. Ludewig S and Chanutin A. Distribution of enzymes in the livers of control and x-irradiated rats, Arch Biochem 1950; 29(2): 441-445
  17. NCP. Management of Persons Accidentally Contaminated with Radionuclides, IAEA Vienna 1980, Report No. 65, 1980; 77
  18. Ohta S, Sakurai N, Sato Y, Inoue T and Shinoda M. Studies on chemical protectors against radiation XXX Radioprotective substances of cnidii rhizoma, Yakugaku Zasshi 1990; 110(10): 746-754 https://doi.org/10.1248/yakushi1947.110.10_746
  19. Orazi A, Du X, Yang Z, Kashai M and Williams DA. Interleukin-11 prevents apoptosis and accelerates recovery of small intestinal mucosa in mice treated with combined chemotherapy and radiation, Lab Invest 1996; 75(1): 33-42
  20. Patchen ML, MacVittie TJ, Williams JL, Schwartz GN and Souza LM. Administration of interleukin-6 stimulates multilineage hematopoiesis and accelerates recovery from radiation-induced hematopoietic depression, Blood 1991; 77(3): 472-480
  21. Potten CS. Interleukin-11 protects the clonogenic stem cells in murine small-intestinal crypts from impairment of their reproductive capacity by radiation, Int J Cancer 1995; 62(3): 356-361 https://doi.org/10.1002/ijc.2910620321
  22. Quan HX and Li HS. Effects of radix Astragali on hemopoiesis in irradiated mice, Zhongguo Zhong Yao Za Zhi 1994; 19(12): 741-743
  23. Schwartz GN, Neta R, Vigneulle RM, Patchen ML and MacVittie TJ. Recovery of hematopoietic colony-forming cells in irradiated mice pretreated with interleukin 1 (IL-1), Exp Hematol 1988; 16(9): 752-757
  24. Travis EL, Fang MZ and Basic I. Protection of mouse bone marrow by WR-2721 after fractionated irradiation, Int J Radiat Oncol Biol Phys 1988; 15(2): 377-382 https://doi.org/10.1016/S0360-3016(98)90019-0
  25. Zneg XL, Li XA and Zhang BY. Immunological and hematopoietic effect of Codonopsis pilosula on cancer patients during radiotherapy, Zhongguo Zhong Xi Yi Jie He Za Zhi 1992; 12(10): 607-608