Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
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Blood Protein Adsorption and Platelet Activation on an Ultra-hydrophilic Substrate

초친수성 표면에서 혈장 단백의 흡착 및 혈소판의 변화에 관한 연구

  • Park, Nam-Hee (Department of Thoracic and Cardiovascular Surgery, Dongsan Medical Center, Keimyung University College of Medicine) ;
  • Chun, Bae-Hyeock (Nano-biotechnology Corporation) ;
  • Je, Hyung-Gon (Department of Thoracic and Cardiovascular Surgery, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Lee, Jun-Wan (Department of Thoracic and Cardiovascular Surgery, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Lee, Jae-Won (Department of Thoracic and Cardiovascular Surgery, Asan Medical Center, College of Medicine, University of Ulsan)
  • 박남희 (계명대학교 의과대학 동산의료원 흉부외과학교실) ;
  • 전배혁 ((주)N.B.T) ;
  • 제형곤 (울산대학교 의과대학 서울아산병원 흉부외과학교실) ;
  • 이준완 (울산대학교 의과대학 서울아산병원 흉부외과학교실) ;
  • 이재원 (울산대학교 의과대학 서울아산병원 흉부외과학교실)
  • Published : 2007.04.05
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Abstract

Background: We evaluate the efficacy of ultra-hydrophilic coated bypass circuits in comparison with uncoated bypass circuits in a porcine cardiopulmonary bypass model. Material and Method: Normothermic cardiopulmonary bypass was peformed in 10 anesthetized pigs via the left atrium and ascending aorta with a centrifugal biopump. Ultra-hydrophilic coated bypass circuits wore used in 5 pigs (the study group) and uncoated bypass circuits were used for the control group. Platelet counts and platelet aggregation tests were peformed. The thrombin-antithrombin(TAT) complex level and total protein level were evaluated. Result: There were no significant changes En the platelet counts and aggregation ability of both groups. The TAT complex levels were not different between the two groups. The total protein level was significantly lower in the control group after cessation of cardiopulmonary bypass. Conclusion: The clinical effects of ultra-hydrophilic coating circuits were not remarkable, in terms of reducing inflammatory reaction and protection of platelet function. However, the effect of protection for blood protein adsorption might be acceptable.

배경: 체외순환으로 발생하는 전신성 염증반응을 줄이기 위해 체외순환 회로의 표면에 초친수성 표면개질을 시행한 후 혈장 단백의 흡착 및 혈소판의 변화를 관찰하였다 대상 및 방법: 60kg 내외의 돼지 10마리를 대상으로 하였다. 체외순환은 좌심방과 상행대동맥에 캐눌라를 거치하여 원심성 바이오펌프를 이용해 2시간 동안 정상체온 하에서 시행하였다. 이때 실험군(n=5)에서는 체외순환회로에 초친수성 표면처리를 하였으며 대조군(n=5)에서는 상용화된 일반적인 튜빙을 사용하였다. 체외순환에 따른 염증반응의 정도를 비교하기 위하여 혈소판 수 및 응집능 검사, 트롬빈-안티트롬빈 복합체 검사, 그리고 혈액 내 총 단백량을 측정하였다. 결과: 양 군에서 모두 혈소판의 수 및 응집능 기능검사에서 유의한 차이를 보이지 않았으며 또한 트롬빈-안티트롬빈 복합체의 농도에서도 두 군 간에 유의한 차이를 보이지 않았다. 그러나 총 단백량의 측정에서는 체외순환 후 대조군에서 실험군에 비해 유의하게 감소되었다. 결론: 초친수성 표면처리는 체외순환 시 혈장 단백의 표면흡착에 따른 혈장 단백의 감소를 줄일 수 있었으나 이로 인한 염증반응의 감소효과는 관찰할 수 없었다.

Keywords

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