Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Molecular Phylogeny of the Amynthas-complex (Oligochaeta: Megascolecidae) Inferred from ITS Nucleotide Sequences

Ribosomal DNA ITS 유전자를 이용한 왕지렁이(빈모강: 지렁이과) 그룹의 계통분류

  • Hong, Yong (Institute of Agricultural Science, Sangju National University) ;
  • James, Samuel W. (Kansas University Natural History Museum and Biodiversity Research Center) ;
  • Hwang, Ui-Wook (Department of Biology, Teachers College, Kyungpook National University) ;
  • Lee, Bo-Eun (Life Science, College of Natural Science, Chung Ang University) ;
  • Park, Soon-Cheol (Life Science, College of Natural Science, Chung Ang University) ;
  • Kim, Tae-Heung (Faculty of Biological Resources Science, College of Agriculture and Life Sciences, Chonbuk National University)
  • 홍용 (상주대학교 농업과학연구소) ;
  • ;
  • 황의욱 (경북대학교 생물교육과) ;
  • 이보은 (중앙대학교 생명과학과) ;
  • 박순철 (중앙대학교 생명과학과) ;
  • 김태흥 (전북대학교 생물자원과학부)
  • Published : 2007.11.30
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Abstract

Phylogeny of the species mainly from the genus Amynthas in family Megascolecidae was inferred at the molecular level using ITS regions in rDNA. With 26 species of earthworms from 10 genera in 2 families, a stretch comprising the 3'-end of the 18S rRNA, ITS1, 5.8S rRNA, ITS2, and 5' end of 28S rRNA was amplified by applying the primers ITS-1, ITS-2. Phylogenetic analyses of nucleotide sequences with a help of MP, NJ, and QP yielded 5 groups similarly. Genus Amynthas was separated largely into two groups, Korean and Philippine origins. Species grouped into the 1st were Amynthas jirensis, A. agrestis, A. gucheonensis, A. sopaikensis, A. bubonis, A. multimaculatus, A. koreanus, A. dageletensis, A. heteropodus, A. odaesanensis, Pontoscolex sp., Pheretima sp. 1, and Dendropheretima banahawensis. Amynthas halconensis, A. isarogensis, A. mindrooensis, Pithemera sp. 2, Pithmera sp. 1, and Pleionogaster sp. clustered into one clade forming the 2nd group. Polypheretima sp. 1 and polypheretima. sp. 2 stayed closely together representing a separate monophyletic status, forming the 3rd group, apart from species in other genera. Archipheretima sp. falls into the 4th group. Distinct morphological characteristics from Archipheretima also coinsides with its branching away from others in the previously reported molecular analyses. Similar to Perionyx excavatus that has been selected as an outgroup, Aporrectodea tuberculata also showed a long branch in the phylogram, but it differed from other 24 species included in the analyses. Unlike others, for example, its habitat is very closely related to that of man.

지렁이과 (family Megascolecidae) 왕지렁이속 (genus Amynthas) 그룹을 rDNA ITS 유전자를 이용하여 계통 유연관계를 알아보고자 하였다. 2과 10속에서 26종의 DNA 염기서열을 이용하여 종간 계통적 유연관계를 MP (Maximum Parsimony), NJ (Neighbor Joining), QP (Quartet Puzzling)로 계통도를 작성하였다. 염기서열이 확보된 Megascolecidae (지렁이과)의 국내외 26종에 대한 계통 분석은 위 3개의 알고리즘 모두 동일한 5개의 그룹으로 나뉘는 것을 확인하였다. 또한 Amynthas 속은 크게 한국산 그룹과 필리핀산 그룹으로 구분되었다. 첫 번째 그룹, Amynthas jirensis, A. agrestis, A. gucheonensis, A. sopaikensis, A. bubonis, A. multimaculatus, A. koreanus, A. dageletensis, A. heteropodus, A. odaesanensis, Pontoscolex sp., Pheretima sp. 1와 다른 그룹, Dendropheretima banahawensis. Amynthas halconensis, A. isarogensis, A. mindrooensis, Pithemera sp. 2, Pithmera sp. 1, and Pleionogaster sp.이다. 이는 ITS 유전자가 속간의 차이를 보여주는 것보다 동일한 속이라 하더라도 지리적 차이에 따른 환경의 영향을 받았음을 알 수 있다. 즉 형태적 분화가 이루어졌다고 하더라도 유전적으로는 동질성을 나타내고 있음을 알 수 있다. 형태적 특징에 의한 명백한 차이는 구분되지 않았다.

Keywords

References

  1. Anderson TJ and RD Adlard. 1994. Nucleotide acid sequence of a rDNA internal transcribed spacer supports synonymy of Saccostrea commercialis and S. glomerata. J. Moll. Stud. 60:196-197 https://doi.org/10.1093/mollus/60.2.196
  2. Baldwin BG, MJ Sanderson, JM Porter, MF Wojciechowski, CS Campbell and MJ Donoghue. 1995. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny. Ann. Mo. Bot. Gard. 82:247-277 https://doi.org/10.2307/2399880
  3. Banks MA, D Hedgecok and C Waters. 1993. Discrimination between closely related Pacific oyster spp. (Crassostrea) via mitochondrial DNA sequences coding for large subunit rRNA. Mol. Mar. Biol. Biotechnol. 2:129-136
  4. Chang CH, SC Chuang, YR Chen and JH Chen. 2005. NADH dehydrogenase subunit 1 gene of the earthworm Amynthas gracilis (Kinberg, 1867) (Oligochaeta: Megascolecidae), with the discussion on inferring the megascolecid phylogeny using DNA sequences. Taiwania. 50:71-79
  5. Chang CH, YH Lin, IH Chen, SC Chuang and JH Chen. 2007. Taxonomic re-evaluation of the Taiwanese montane earthworm Amynthas wulinensis Tsai, Shen & Tsai, 2001 (Oligochaeta: Megascolecidae): Polytypic species or species complex?. Organ. Diversity Evol. 25062:1-10
  6. Easton EG. 1979. Pheretima group (Megascolecidae: Oligochaeta): Archipheretima, Metapheretima, Planapheretima, Pleionogaster and Polypheretima. Bull. Br. Mus. Nat. Hist. (Zool.). 35:1-126
  7. Edwards CA. 1988. Breakdown of animal, vegetable, and industrial organic wastes by earthworms. Agric. Ecosyst. Environ. 24:21-31 https://doi.org/10.1016/0167-8809(88)90053-9
  8. Hong Y and SW James. 2001a. New species of Korean Amynthas (Oligochaeta, Megascolecidae) with two pairs of spermathecae. Rev. Suisse Zool. 108:65-93 https://doi.org/10.5962/bhl.part.79621
  9. Hong Y and SW James. 2001b. Five new earthworms of the genus Amynthas Kinberg (Megascolecidae) with four pairs of spermathecae. Zool. Stud. 40:269-275
  10. Hong Y, TH Kim and YE Na. 2001. Identity of two earthworms used in vermiculture and vermicomposting in Korea: Eisenia andrei and Perionyx excavatus. Korean J. Syst. Zool. 17:185-190
  11. Hong Y. 2002. New earthworms of the genus Drawida Michaelsen, 1900 (Oligochaeta: Moniligastridae) from Korea. Rev. Suisse Zool. 109:475-482
  12. Hong Y and TH Kim. 2002a. Three new earthworms of the genus Amynthas (Megascolecidae) from Mt. Gyeryong, Korea. Rev. Suisse Zool. 109:483-489 https://doi.org/10.5962/bhl.part.79603
  13. Hong Y and TH Kim. 2002b. Four new earthworms of the genus Amynthas (Oligochaetae: Megascolecidae) from Korea. Korean J. Biol. Sci. 6:195-199 https://doi.org/10.1080/12265071.2002.9647651
  14. Hong Y and TH Kim. 2004. New species of Amynthas Kinberg, 1867 from the Philippines (Oligochaeta: Megascolecidae). Rev. Suisse Zool. 111:729-741 https://doi.org/10.5962/bhl.part.80266
  15. Hong Y and TH Kim. 2005. The earthworm fauna from Ulleungdo Isaland, Korea with one new species of the genus Amynthas (Oligochaeta: Megascolecidae). Korean J. Syst. Zool. 21:129-136
  16. Jameieson BGM, S Tiller, A Tiller, J-L Justine, E Ling, S James, K McDonald and AF Hugall. 2002. Phylogeny of the Megascolecidae (Oligochaeta, Annelida): Combined versus partitioned analysis using nuclear (28s) and mitochondrial (12s, 16s) rDNA. Zoosystema 24: 707-734
  17. James SW. 2004. New species of Amynthas, Pheretima and Pleionogaster (Clitellata: Megascolecidae) of the Mt. Kitanglad range, Mindanao Island, Philippines. Raf. Bull. Zool. 52:289-313
  18. James SW. 2005a. New genera and species of pheretimoid earthworms (Clitellata: Megascolecidae) from southern Luzon, Philippines. Syst. and Biodiver. 2:271-279 https://doi.org/10.1017/S1477200004001446
  19. James SW. 2005b. Preliminary molecular phylogeny in the Pheretima group of genera (Crassiclitellata: Megascolecidae) using Bayesian analysis. Advances in Earthworm Taxonomy II (Annelida: Oligochaeta). pp. 129-142
  20. Kobayashi S. 1936. Earthworms from Koryo, Korea. Sci. Rep. Tohoku Univ. 11:139-184
  21. Kobayashi S. 1938. Earthworms of Korea I. Sci. Rep. Tohoku Imp. Univ. Biol. 13:89-170
  22. Reynolds JW and DG Cook. 1976. Nomenclatura Oligochaetologica, a catalogue of names, descriptions and type specimens of the Oligochaeta. Fredericton: Univ. of New Brunswick. 217pp
  23. Sims RW and EG Easton. 1972. A numerical revision of the earthworm genus Pheretima auct. (Megascolecidae: Oligochaeta) with the recognition of new genera and an appendix on the earthworms collected by the Royal Society North Borneo Expedition. Biol. J. Linn. Soc. 4:169-268 https://doi.org/10.1111/j.1095-8312.1972.tb00694.x
  24. Song MJ and KY Paik. 1973. Earthworms from Mt. Sopaik, Korea. Korean J. Zool. 16:5-12
  25. Swofford DL. 2002. PAUP*: Phylogenetic analysis using parsimony and other method. Version 4.0b10. Sinauer Associates, Sunderlad
  26. Thai TB, 1983. Evolutionary changes in the set of seminal receptacles and development of the way of sperm transfer in Pheretima (Oligochaeta: Megascolecidae). Zool. Zurn. 62:1457-1461
  27. Tompson JD, DG Higgins and TJ Gibson. 1997. Clustal W: Improving sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acid Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  28. Yang YW, PF Tseng, PY Tai and CJ Chang. 1998. Phylogenetic position of Raphanus in relation to Brassica species based on 5S rRNA spacer sequence data. Bot. Bull. Acad. Sin. 39:153-160