Maxillofacial Plastic and Reconstructive Surgery

  • 제29권6호
  • /
  • Pages.494-498
  • /
  • 2007
  • /
  • 2288-8101(pISSN)
  • /
  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

조골세포에서 pleiotrophin(PTN)의 발현에 대한 연구

PLEIOTROPHIN (PTN) EXPRESSION IN OSTEOBLASTIC CELLS

  • Kim, Byeong-Yol (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Rim, Jae-Suk (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Kwon, Jong-Jin (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Jang, Hyon-Seok (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Lee, Eui-Seok (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Jun, Sang-Ho (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University) ;
  • Kim, Young-Jin (Department of Oral & Maxillofacial Surgery, School of Medicine, Korea University)
  • 발행 : 2007.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Pleiotrophin or osteoblast-specific factor 1(HOSF-1) is a growth-associated protein present in bone matrix. This study was designed to study pleiotrophin expression in osteoblastic cells. Pleiotrophin was expressed by osteoblast-like cell line. Pleiotrophin expression increased following the proliferative phase and was minimal at the terminal phases of the induced differentiation of cultured MC3T3-E1 cells. Pleiotrophin expression represents another autocrine factor that may contribute to the physiologic control of induced bone formation. In this study, induced osteogenesis will be examined in the context of the osteoblast expression of and regulation by PTN. I hypothesized that PDGF-BB stimulation of PTN expression represents an important paracrine signal during the induced osteogenesis associated with periodontal and implant surgeries. The possible mediation by PTN of anabolic effects attributed to PDGF-BB stimulation was examined in cell culture models of osteoblast differentiation. These studies will contribute fundamental insights to osteoblast biology and insights regarding the potential use of factors such as PTN in the clinical environment.

키워드

참고문헌

  1. Simmons DJ, Grynpas M : Mechanisms of Bone formation in vivo, Hall BK (Ed) Bone, Telford Press Inc Caldwell NJ, 1990
  2. Bonewald LF, Mundy CR : Role of transforming growth factor beta in bone remodeling: A review. Connective Tissue Res 23 : 201, 1989 https://doi.org/10.3109/03008208909002418
  3. Canalis E, McCarthy TL, Centrella M : Growth factors and cytokines. Bone cell metabolism. Annu Rev Med 42 : 17. 1991 https://doi.org/10.1146/annurev.me.42.020191.000313
  4. Zhou HY, Ohnuma Y, Takita H et al : Effects of a bone lysine-rich 18 kDA protein on osteoblast-like MC3T3-E1 cells. Biochem Biophys Res Comm 186 : 1288, 1992 https://doi.org/10.1016/S0006-291X(05)81545-1
  5. Kuo MD, Oda Y, Huang JS et al : Amino acid sequence and characterization of a Heparin-binding neurite-promoting factor (p18) from bovine brain. J Biol Chem 265 : 18749, 1990
  6. Milner PG, Li YS. Hoffman RM et al : A novel 17 KD heparin-binding growth factor (HBGF-8) in bovine uterus : purification and N-terminal amino acid sequence. Biochem Biophys Res Comm 165 : 1096, 1989 https://doi.org/10.1016/0006-291X(89)92715-0
  7. Li YS, Milner PG, Chauhan AK et al : Cloning and expression of a developmentally regulated protein that induces mitogenic and neurite outgrowth activity. Science 250 : 1690. 1990 https://doi.org/10.1126/science.2270483
  8. Tezuka KI, Takeshita S, Hakeda Y et al : Isolation of Mouse and Human cDNA Clones Encoding a Protein Expressed Specifically in Osteoblasts and Brain Tissues. Biochem Biophys Res Comm 173 : 246, 1990 https://doi.org/10.1016/S0006-291X(05)81048-4
  9. Katoh KI. Takeshita S. Sato M et al : Genomic organization of the mouse OSF-1 Gene. DNA and Cell Biol 11: 735. 1992 https://doi.org/10.1089/dna.1992.11.735
  10. Milner PG. Shah D, Veile R et al : Cloning, nucleotide sequence, and chromosome location of the human pleiotrophin gene. Biochemistry 31 : 12023, 1992 https://doi.org/10.1021/bi00163a009
  11. Li YS, Hoffman RM, Le Beau MM et al : Characterization of the human pleiotrophin gene Promoter region and chromosomal localization. J Biol Chem 267 : 26011, 1992
  12. Tomomura M. Kadomatso K, Matsubara S et al : A retinoic acid responsive gene MC found in the teratocarcinoma system heterogeneity of the transcript and the nature of the translation product. J Biol Chem 265 : 10765, 1990
  13. Muramatsu T : Midkine(MK) the product of a retinoic acid responsive gene and pleiotrophin constitute a new protein family regulating growth and differentiation. Int J Devel Biol 37 : 183. 1993
  14. Kreschner PJ. Fairhurst JL. Decker MM et al : Cloning characterization and developmental regulation of two members of a novel human gene family of neurite outgrowth promoting proteins. Growth factors 5 : 99, 1991 https://doi.org/10.3109/08977199109000275
  15. Mitsiadis TA, Salmivirta M, Muramatsu T et al Expression of the heaprin-binding cytokines midkine(MK) and HB-GAM(pleiotrophin) is associated with epithelial-mesemchymal interactions during fetal development and organogenesis. Development 121 : 37, 1995
  16. MerenmiesO J. Rauvala H : Molecular Cloning of the 18 kDa growth-associated protein of developing brain. J Biol Chem 265 : 16721, 1990
  17. Neame PJ. Young CN, Brock CWQ et al : Pleiotrophin is an abundant protein in dis associative extracts of bovine fetal epiphyseal cartilage and nasal cartilage from newborns. J Orthop Res 11 : 479. 1992 https://doi.org/10.1002/jor.1100110403
  18. Sudo H. Kodama H, Amagai Y et al : In vitro differentiation and calcification in a new clonal osteogenic cell line derived from new born mouse calvaria, J Cell Biol 96 : 192. 1983
  19. Quarles LP, Yohay DA. Lever LW et al : Distinct proliferature and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. J Bone Miner Res 7 : 683. 1992
  20. Chomczynski P. Sacchi N : Single-step Method of RNA Isolation by Guanidinium Thiocyanate-Phenol-Chloroform Extraction. Analytical Biochem 162 : 156. 1987
  21. Sambrook J. Fritsch EF. Maniatis T : Molecular Cloning. A laboratory Manual. 2nd ed. Cold Spring Harbor. 1989
  22. Mitlak B. Finkelman R. Hill E et al : The Effect of Systemically Administered PDGF-BB on the Rodent Skeleton. Journal of Bone and Mineral Research 11(2) : 238. 1996
  23. Li YS. Gurrieri M. Deuel TF : Pleiotrophin gene expression is highly restricted and is regulated by platelet-derived growth factor. Biochem Biophys Res Commun 15 : 184(1) : 427. 1992 https://doi.org/10.1016/0006-291X(92)91211-8
  24. Gay CG. Winkles JA : Heparin-binding growth factor-1 stimulation of human endothelial cells induces platelet-derived growth factor A-chain gene expression. J Biol Chem 25 : 265(6) : 3284. 1990
  25. Jaulo E. Julkunen I. Merenmies J et al : Secretion and biological activities of heparin-binding growth-associated molecule Neurite outgrowth- promoting and mitogenic actions of the recombinant and tissue-derived protein. J Biol Chem 267 : 11408. 1992
  26. Laaroubi L. Delbe J. Vacherot F et al : Mitogenic and in vitro angiogenic activity of human recombinant heparin affinity regulatory peptide. Growth Factors 10 : 89. 1994 https://doi.org/10.3109/08977199409010982
  27. Chauhan AK, Li YS. Deuel TF : Pleiotrophin transforms NIH 3T3 cells and induces tumors in nude mice. Proc Nat'l Acad Sci USA 90 : 679. 1993
  28. Czubayko F. Riegel AT. Wellstein A : Ribozyme-targeting elucidates a direct role of pleiotrophin in tumor growth. J Biol Chem 269 : 21358. 1994
  29. Gieffers C. Engelhardt W. Brenzel G et al : Receptor binding of Osteoblast -specific factor 1 (OSF-1/HB-GAM) to human osteosarcoma cells promotes cell attachment. Eur J Cell Biol 62 : 352. 1993
  30. Raulo E. Chernousov MA. Carey DJ et al : Isolation of a neuronal cell surface receptor of heparin binding growth-associated molecule(HB-GAM). Identification as N-syndecan(syndecan-3). J Biol Chem 269 : 12999. 1994