Maxillofacial Plastic and Reconstructive Surgery

  • 제29권6호
  • /
  • Pages.474-484
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  • 2007
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
대한악안면성형재건외과학회 (Korean Association of Maxillofacial Plastic and Reconstructive Surgeons)
권호 표지

가토의 골수 세포에서 분화된 골모세포의 골 형성에 혈소판 농축 혈장이 미치는 효과: 조직 형태학적 분석

EFFECT OF PLATELET-RICH PLASMA ON OSTEOGENESIS OF MARROW-DERIVED OSTEOBLASTS IN THE MANDIBLE OF RABBIT: HISTOMORPHOMETRIC ANALYSIS

  • 박영주 (한림대학교 의료원 강남성심병원 구강악안면외과학교실) ;
  • 신진업 (한림대학교 의료원 강남성심병원 구강악안면외과학교실) ;
  • 정재안 (한림대학교 의료원 강남성심병원 구강악안면외과학교실) ;
  • 전민수 (한림대학교 의료원 강남성심병원 구강악안면외과학교실) ;
  • 김보균 (한림대학교 의료원 강남성심병원 구강악안면외과학교실) ;
  • 송준호 (한림대학교 의료원 강남성심병원 구강악안면외과학교실) ;
  • 연병무 (한림대학교 의료원 강남성심병원 구강악안면외과학교실) ;
  • 임성철 (조선대학교 의과대학 병리학교실) ;
  • 강태인 (한림대학교 의료원 강남성심병원 구강악안면외과학교실)
  • Park, Young-Ju (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center) ;
  • Shin, Jin-Eob (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center) ;
  • Chung, Jae-An (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center) ;
  • Jeon, Min-Su (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center) ;
  • Kim, Bo-Gyun (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center) ;
  • Song, Jun-Ho (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center) ;
  • Yeon, Byong-Moo (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center) ;
  • Lim, Sung-Chul (Department of Pathology, Chosun University Hospital) ;
  • Gang, Tae-In (Department of Oral and Maxillofacial Surgery, Kang-Nam Sacred Heart Hospital, Hallym Medical Center)
  • 발행 : 2007.11.30
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초록

Purpose: The effect of platelet-rich plasma(PRP) on osteogenesis of marrow-derived osteoblasts on histomorphometric analysis in the mandible of rabbit was assessed. Materials and Method: Bone marrow cells were obtained from iliac bone of rabbits and were cultured in a Dulbecco's Modified Eagle's Medium(DMEM) with Dexamethasone, L-Ascortic acid, ${\beta}$-Glycerophosphate to proliferate and differentiate into osteoblasts for $4{\sim}5$ weeks. The expression of osteogenic mar-kers was detected by reverse transcription-polymerase chain reaction(RT-PCR) and silver nitrate stain. Then we prepared bony defects in the mandible of rabbit, 10.0mm in diameter and 4.0mm deep, by trephine bur. In the control group, the defects were filled with autogenous bone and cultured osteoblasts. In the experimental group, the defects were filled with autogenous bone, cultured osteoblasts and PRP. 2 weeks, 4 weeks, 8 weeks later, each group was evaluated with histological and histomorphometric analyses. Results: In vitro, osteoblasts were identified on RT-PCR and silver nitrate stain. According to histological observation, at 2 weeks well-developed anasto-mosing newly-formed woven bone was observed, at 4 weeks anastomosing newly-formed woven bone having osteoblastic activation was observed, and at 8 weeks thick newly-formed woven bone was observed in both control and experimental groups. According to histomorphometric analysis, there were 1.5% more newly-formed bone volume in experimental group than control group at 2 weeks, 28.4% more at 4 weeks, 4.3% more at 8 weeks. Particularly there were significant differences in bone volume at 4 weeks and 8 weeks new bone. Conclusion: Our results demonstrated PRP may enhance osteogenesis of marrow-derived osteoblasts at 4 weeks, 8 weeks.

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