The Diversity of Multi-drug Resistance Profiles in Tetracycline-Resistant Vibrio Species Isolated from Coastal Sediments and Seawater

  • Neela Farzana Ashrafi (Center for Marine Environmental Studies (CMES), Ehime University) ;
  • Nonaka Lisa (Center for Marine Environmental Studies (CMES), Ehime University) ;
  • Suzuki Satoru (Center for Marine Environmental Studies (CMES), Ehime University)
  • Published : 2007.02.22

Abstract

In this study we examined the multi-drug resistance profiles of the tetracycline (TC) resistant genus Vibrio to determine its susceptibility to two ${\beta}-lactams$, ampicillin (ABPC), and mecillinam (MPC), as well as to macrolide, erythromycin (EM). The results showed various patterns of resistance among strains that were isolated from very close geographical areas during the same year, suggesting diverse patterns of drug resistance in environmental bacteria from this area. In addition, the cross-resistance patterns suggested that the resistance determinants among Vibrio spp. are acquired differently within the sediment and seawater environments.

Keywords

References

  1. Furushita, M., T. Shiba, T. Maeda, M. Yahata, A. Kaneoka, Y. Takahashi, K. Torii, T. Hasegawa, and M. Ohta. 2003. Similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates. Appl. Environ. Microbiol. 69, 5336- 5342 https://doi.org/10.1128/AEM.69.9.5336-5342.2003
  2. George, A.M. 1996. Multidrug resistance in enteric and other Gram-negative bacteria. FEMS Microbiol. Lett. 139, 1-10 https://doi.org/10.1111/j.1574-6968.1996.tb08172.x
  3. Kim, S.R., L. Nonaka, M.J. Oh, C.R. Lavilla-Pitogo, and S. Suzuki. 2003. Distribution of an oxytetracycline resistance determinant tet(34) among marine bacterial isolates of a Vibrio species. Microbes Environ. 18, 74-81 https://doi.org/10.1264/jsme2.18.74
  4. Kim, S.R., L. Nonaka, and S. Suzuki. 2004. Occurrence of tetracycline resistance genes tet(M) and tet(S) in bacteria from marine aquaculture sites. FEMS Microbiol. Lett. 237, 147-156 https://doi.org/10.1111/j.1574-6968.2004.tb09690.x
  5. Mazel, D., B. Dychinco, V.A. Webb, and J. Davies. 1998. A distinctive class of integron in the Vibrio cholerae genome. Science 280, 605-608 https://doi.org/10.1126/science.280.5363.605
  6. Muyzer, G., E.C. de Wall, and A.G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerage chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59, 695- 700
  7. Nikaido, H. 1994. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 264, 382-388 https://doi.org/10.1126/science.8153625
  8. Nonaka, L., T. Isshiki, and S. Suzuki. 2000. The occurrence of oxytetracycline resistant bacteria in the fish intestine and the seawater environment. Microbes Environ. 15, 223-228 https://doi.org/10.1264/jsme2.2000.223
  9. Paul, J.H., M.E. Frischer, and J.M. Thurmond. 1991. Gene transfer in marine water column and sediment microcosms by natural plasmid transformation. Appl. Environ. Microbiol. 57, 1509-1515
  10. Roberts, M.C. 2005. Update on acquired tetracycline resistance genes. FEMS Micribiol. Lett. 245, 195-203 https://doi.org/10.1016/j.femsle.2005.02.034
  11. Sambrook, J. and D.W. Russell. 2001. Molecular cloning: a labotary manual, 3rd (eds.), vol. 1, p. 1.32-1.34, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA
  12. Samuelsen, O.B., V. Torsvik, and A. Ervik. 1992. Long-range changes in oxytetracycline concentration and bacterial resistance toward oxytetracycline in a fish farm sediment after medication. Sci. Total Environ. 114, 25-36 https://doi.org/10.1016/0048-9697(92)90411-K
  13. Sayah, R.S., J.B. Kaneene, Y. Johnson, and R.A. Miller. 2005. Patterns of antimicrobial resistance observed in Escherichia coli isolates obtained from domestic-and wild-animal fecal samples, human septage, and surface water. Appl. Environ. Microbiol. 71, 1394-1404 https://doi.org/10.1128/AEM.71.3.1394-1404.2005
  14. Schmidt, A.S., M.S. Bruun, I. Dalsgaard, K. Pedersen, and J.L. Larsen. 2000. Occurrence of antimicrobial resistance in fishpathogenic and environmental bacteria associated with four Danish rainbow trout farms. Appl. Environ. Microbiol. 66, 4908-4915 https://doi.org/10.1128/AEM.66.11.4908-4915.2000
  15. Shannon, C.C. and W. Weaver. 1949. The Mathematical Theory of Communication. Urbana, University of Illinois Press. p. 117
  16. Walsh, C. 2003. Natural and producer immunity versus acquired resistance, p. 91-105. In Antibiotics: actions, origins, resistance. ASM Press, Washington D.C., USA