Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Morphology and Adhesion of Campylobacter jejuni to Chicken Skin Under Varying Conditions

  • Jang, Keum-Il (Department of Food Science and Technology and Research Center for Bioresources and Health, Chungbuk National University) ;
  • Kim, Min-Gon (NanoBiotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University) ;
  • Lee, Kyu-Ho (Environmental Science, Hankuk University of Foreign Studies) ;
  • Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, Cheorl-Ho (Department of Biological Science, Sungkyunkwan University) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology and Research Center for Bioresources and Health, Chungbuk National University)
  • Published : 2007.02.28
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Abstract

The adhesion of Campylobacter jejuni to chicken skin, along with the associated morphological changes under aerobic conditions at 4, 25, and $37^{\circ}C$ and microaerobic ($O_2\;5%,\;CO_2\;10%,\;N_2\;85%$) conditions, were investigated using confocal laser scanning microscopy (CLSM), flow cytometry, and plate counting. The morphological change of C. jejuni from a spiral shape to a coccoid form or VBNC form (viable but nonculturable form) progressed rapidly under aerobic conditions at 25, 37, and $4^{\circ}C$. As regards adhesion, the C. jejuni cells were mostly located in the crevices and feather follicles of the chicken skin, where the cells in the feather follicles floated freely in the entrapped water, even after the skin was rinsed quite thoroughly. CLSM also revealed the penetration of some spiral-shaped C. jejuni cells into the chicken skin. Even after changing their shape at various temperatures, coccoid-form C. jejuni cells were still found in the crevices and feather follicles of the chicken skin.

Keywords

References

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