Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Molecular Parameters for Assessing Marine Biotoxicity: Gene Expressions of Rockfish (Sebastes schlegeli) Exposed to Polycyclic Aromatic Hydrocarbons

  • Woo, Seon-Ock (Southern Coastal Environment Research Division, Korea Ocean Research and Development Institute) ;
  • Yum, Seung-Shic (Southern Coastal Environment Research Division, Korea Ocean Research and Development Institute) ;
  • Park, Hong-Seog (Genome Research Center, Korea Research Institute of Bioscience and Technology) ;
  • Jung, Jee-Hyun (Southern Coastal Environment Research Division, Korea Ocean Research and Development Institute) ;
  • Lee, Suk-Chan (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Kim, So-Jung (Gyeongbuk Institute for Marine Bioindustry) ;
  • Lee, Taek-Kyun (Southern Coastal Environment Research Division, Korea Ocean Research and Development Institute)
  • Published : 2007.12.31
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Abstract

Environmental and anthropogenic changes affect the health and stability of marine ecosystem. In this study we aimed to identify molecular biomarkers for ecotoxicological pollutants risk assessment in the rockfish (Sebastes schlegeli). We designed primers based on conserved sequences by multiple alignments of target genes from related species, and cloned the partial cDNAs of cytochrome P450 (CYP1A1), glutathione S-transferase (GST), metallothionein (MT), superoxide dismutase (SOD), ubiquitin (UB), vitellogenin (VTG) and $\beta$-actin by reverse transcription polymerase chain reaction (RT-PCR) from S. schlegeli. Northern blot results indicated that these six genes expressions were significantly induced by benzo[a]pyrene (BaP, 1 ${\mu}M$) and that the level of each of their transcripts increased in BaP-exposed rockfish in a time-dependent manner. This study suggests that transcriptional changes in these six genes may be used for monitoring environmental exposure to polycyclic aromatic hydrocarbons (PAHs).

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References

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