Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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The Expression Changes of Casein mRNAs in Mammary Epithelial Cells Recovered from Bovine Milk during the Lactation Period

  • Received : 2006.03.23
  • Accepted : 2007.02.20
  • Published : 2007.06.01
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Abstract

The aim of this study was to examine the correlation between bovine casein (CN) mRNA expression levels in mammary epithelial cells and lactation period, the yields of milk proteins and other parameters. The cells were collected from each cow's milk, which contained somatic cell counts (SCC) of less than 100,000 cells/ml. The levels of ${\alpha}s1-$, ${\alpha}s2-$, ${\beta}$- and ${\kappa}$-CN mRNA expression were significantly correlated with each other in mammary epithelial cells (p<0.01). All cows produced either less than 30 kg/day/cow or a over 30 kg/day/cow level of milk yield (MY). It was shown that the CN mRNA expression levels decreased gradually from the calving period to late lactation, when MY was over 30 kg/day/cow. The SCC tended to increase gradually during the course of lactation, but it was negatively correlated with milk protein and CN yields (p<0.01) when MY was less than 30 kg/day/cow. Moreover, there was a tendency for a negative correlation between SCC and ${\alpha}s1$-CN and ${\beta}$-CN mRNA expression level, when MY was less than 30 kg/day/cow (p<0.05).

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References

  1. Auldist, M. J., B. J. Walsh and N. A. Thomson 1998. Seasonal and lactational influences on bovine milk composition in New Zealand. J. Dairy Res. 65:401-411. https://doi.org/10.1017/S0022029998002970
  2. Didier, A. and R. M. Bruckmier. 2004. mRNA expression of apoptosis-related genes in mammary tissue and milk cells in response to lipopolysaccharide challenge and durig subclinical mastitis. Milchwissenschaft. 59(2):119-123.
  3. Grosclaude, F., P. Joudrier and M-F. Mahe. 1979. A genetic and biochemical analysis of a polymorphism of bovine $\alpha$s2-casein. J. Dairy Res. 46(2):211-213. https://doi.org/10.1017/S0022029900017052
  4. Kuraishi, T., Y. Sun, F. Aoki, K. Imakawa and S. Sakai. 2000. The poly (A) tail length of casein mRNA in the lactating mammary gland changes depending upon the accumulation and removal of milk. Biochem. J. 347:579-583. https://doi.org/10.1042/0264-6021:3470579
  5. Mercier, J-C. and J-L. Vilotte. 1993. Structure and function of milk protein genes. J. Dairy Sci. 76(10):3079-3098. https://doi.org/10.3168/jds.S0022-0302(93)77647-X
  6. Ng-Kwai-Hang, K. F., J. F. Hayes, J. F. Moxley and H. G. Monardes. 1987. Variation in milk protein concentrations associated with genetic polymorphism and environmental factors. J. Dairy Sci. 70(3):563-570. https://doi.org/10.3168/jds.S0022-0302(87)80042-5
  7. Schmitz, S., M. V. Pfaffl, M. Miller, J. Buchberger, T. Meyer, H. Sauerwein and R. M. Bruckmaier. 2004. mRNA expression of immune factors and milk proteins in mammary tissue and milk cells and their concentration in milk during subclinical mastitis. Milchwissenschaft. 59(7-8):351-355.
  8. Travers, M. T., M. C. Barber, E. Tonner, L. Quarrie, C. J. Wilde and D. J. Flint. 1996. The role of prolactin and growth hormone in the regulation of casein gene expression and mammary cell survival: Relationships to milk synthesis and secretion. Endocrinol. 137(5):1530-1539. https://doi.org/10.1210/en.137.5.1530