Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Cloning and Expression of the Duck Leptin Gene and the Effect of Leptin on Food Intake and Fatty Deposition in Mice

  • Dai, Han Chuan (College of Animal Science, Huazhong Agricultural University) ;
  • Long, Liang Qi (College of Animal Science, Huazhong Agricultural University) ;
  • Zhang, Xiao Wei (College of Animal Science, Huazhong Agricultural University) ;
  • Zhang, Wei Min (College of Animal Science, Huazhong Agricultural University) ;
  • Wu, Xiao Xiong (College of Animal Science, Huazhong Agricultural University)
  • Received : 2006.09.21
  • Accepted : 2007.01.17
  • Published : 2007.06.01
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Abstract

Leptin is the adipocyte-specific product of the obese gene and plays a major role in food intake and energy metabolism. Leptin research was mainly focused on mammalian species, but understanding of leptin and its function in poultry is very poor. In this study, the duck leptin gene was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) from duck liver RNA. The cDNA fragment was inserted into the pET-28a expression vector, and the resulting plasmid was expressed in Escherichia coli BL21 (DE3). Experimental mice were given an intraperitoneal injection of 10 mg/kg leptin dissolved in phosphate buffered saline (PBS), while the control mice were injected with PBS. The effect of leptin on food intake, body weight and fatty deposition in mice was detected. Sequence analysis revealed that duck leptin had a length of 438 nucleotides which encoded a peptide with 146 amino acid residues. The sequence shares highly homology to other animals. The coding sequence of duck leptin was 84 and 86% identical to human and pig leptin nucleotides sequence. Highest identity was with the rat coding sequence (95%). The identity of the amino acid sequence was 84, 82 and 96% respectively compared to that of the human, pig and rat. Results of SDS-PAGE analysis indicated that a fusion protein was specifically expressed in E. coli BL21 (DE3). The purified product was found to be biologically active during tests. Continuous administration of recombinant duck leptin inhibited food intake. Despite the decrease of food intake, leptin significantly induced body weight and fatty deposition. These changes were accompanied by a significant down-secretion of plasma glucose, cholesterol, triglyceride and insulin levels in mice. The observations provide evidence for an inhibitory effect of leptin in the regulation of food intake and for a potential role of duck leptin in the regulation of lipogenesis.

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References

  1. Ahima, R. S and J. S. Flier. 2000. Leptin. Annu. Rev. Physiol. 62:413-437. https://doi.org/10.1146/annurev.physiol.62.1.413
  2. Ashwell, C. M., S. M. Czerwinski, D. M. Brocht and J. P. McMurtry. 1999. Hormonal regulation of leptin expression in broiler chickens. Am. J. Physiol. 276:R226-232.
  3. Bryson, J. M., J. L. Phuyal, V. Swan and I. D. Caterson. 1999. Leptin has acute effects on glucose and lipid metabolism in both lean and gold thioglucose-obese mice. Am. J. Physiol. 270:E417-422.
  4. Cassy, S., M. Picard, S. Crochet, M, Derouet, D. H. Keisler and M. Taouis. 2004. Peripheral leptin effect on food intake in young chickens is influenced by age and strain. Domest. Anim. Endocrinol. 27:51-61. https://doi.org/10.1016/j.domaniend.2004.01.004
  5. Denbow, D. M., S. Meade, A. Robertson, J. P. McMurtry, M. Richards and C. M. Ashwell. 2000. Leptin-induced decrease in food intake in chickens. Physiol. Behav. 69:359-362. https://doi.org/10.1016/S0031-9384(99)00258-9
  6. Dridi, S., J. Buyse, E. Decuypere and M. Taouis. 2005. Potential role of leptin in increase of fatty acid synthase gene expression in chicken liver. Domest. Anim. Endocrinol. 29:646-660. https://doi.org/10.1016/j.domaniend.2005.05.002
  7. Dridi, S., N. Raver, E. E. Gussakovsky, M. Derouet, M. Picard, A. Gertler and A. Taaouis. 2000. Biological activities of recombinant chicken leptin C4S analog compared with unmodified leptons. Am. J. Physiol. Endocrinol. Metab. 279:E116-123. https://doi.org/10.1152/ajpendo.2000.279.1.E116
  8. Elmquist, J. K., R. S. Ahima, C. F. Elias, J. S. Flier and C. B. Saper. 1998. Distributions of leptin receptor mRNA isoforms in the rat brain. J. Comp. Neurol. 395:535-547. https://doi.org/10.1002/(SICI)1096-9861(19980615)395:4<535::AID-CNE9>3.0.CO;2-2
  9. Fei, H., H. J. Okano, C. Li, G. H. Lee, C. Zhao, R. Darnell and J. M. Friedman. 1997. Anatomic localization of alternatively splicedleptin receptors (ob-R) in mouse brain and other tissues. Proc. Natl. Acad. Sci. USA. 94:7001-7005. https://doi.org/10.1073/pnas.94.13.7001
  10. Freidman, J. M. and J. L. Halaas. 1998. Leptin and the regulation of body weight in mammals. Nature. 395:763-770. https://doi.org/10.1038/27376
  11. Fukuda, H., N. Iritani, T. Sugimoto and H. Ikeda. 1999. Transcriptional regulation of fatty acid synthase gene by insulin/glucose, polyunsaturated fatty acid and leptin in hepatocytes and adipocytes in normal and genetically obese rats. Eur. J. Biochem. 260:505-511.
  12. Halaas, J. L., K. S. Gajiwala, M. Maffei, S. L. Cohen, B. T. Chait, D. Rabinowitz, R. L. Lallone, S. K. Burley and J. M. Friedman. 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Sci. 269:543-546. https://doi.org/10.1126/science.7624777
  13. Horev, G., P. Einat, T. Aharoni, Y. Eshdat and M. Friedman-Einat. 2000. Molecular cloning and properties of the chicken leptin-receptor gene. Mol. Cell. Endocrinol. 162:95-106. https://doi.org/10.1016/S0303-7207(00)00205-7
  14. Leveille, G. A., E. K. O'hea and K. Chakrabarty. 1968. In vivo lipogenesis in the domestic chicken. Proc. Soc. Exp. Biol. Med. 128:398-401. https://doi.org/10.3181/00379727-128-33022
  15. Lin, X., V. H. Volko., Y. Narnaware, N. J. Bernier, P. Peyon and R. E. Peter. 2000. Review: Brain regulation of feeding behavior and food intake in fish. Comp. Biochem. Physiol. A. 126:415-434.
  16. Lohmus, M., L. F. Sundstrom, M. El Halawani and B. Silverin. 2003. Leptin depresses food intake in great tits (Parus major). Gen. Comp. Endocrinol. 13:57-61.
  17. Lopez-Soriano, J., N. Carbo, F. J. Lopez-Soriano and J. M. Argil'es. 1998. Short-term effects of leptin on lipid metabolismin the rat. FEBS Lett. 431:371-374. https://doi.org/10.1016/S0014-5793(98)00784-4
  18. McMurtry, J. P., C. Ashwell, M. Richards and R. Vasilatos- Younken. 2002. Leptin: molecular biology and physiology (Ed. A. Dawson, C. M. Chaturvedi). Avian endocrinology. New Dehli, India: Narosa Publishing House.pp. 317-328.
  19. Mistry, A. M., A. G. Swick and D. R. Romsos. 1997. Leptin rapidly lowers food intake and elevates metabolic rates in lean and ob/ob mice. J. Nutr. 127:2065-2072. https://doi.org/10.1093/jn/127.10.2065
  20. Mizuno, T. M., S. P. Kleopoulos, H. T. Bergen, J. L. Roberts, C. A. Priest and C. V. Mobbs. 1998. Hypothalamic proopiomel-anocortinm RNA is reduced by fasting in ob/ob and db/db mice, but is stimulated by leptin. Diabetes. 47:294-297. https://doi.org/10.2337/diabetes.47.2.294
  21. Moussavi, A. H., M. Ahouei, M. R. Nassiry and A. Javadmanesh. 2006. Association of Leptin Polymorphism with Production, Reproduction and Plasma Glucose Level in Iranian Holste in cows. Asian-Aust. J. Anim. Sci. 19(5):627-631. https://doi.org/10.5713/ajas.2006.627
  22. Muller, G., J. Ertl, M. Gerl and G. Preibish. 1997. Leptin impairs metabolic actions of insulin in isolated rat adipocytes. J. Biol. Chem. 272:10585-10593. https://doi.org/10.1074/jbc.272.16.10585
  23. Muruzabal, F. J., G. Fruhbeck, J. Gomez-Ambrosi, M. Archanco and M. A. Burrel. 2002. Immunocytoc-hemical detection of leptin in non-mammalian vertebrate stomach. Gen. Comp. Endocrinol. 128:149-152. https://doi.org/10.1016/S0016-6480(02)00072-2
  24. Ohkubo, T., M. Tanaka and K. Nakashima. 2000. Structure and tissue distribution of chicken leptin receptor (cOb-R) mRNA. Biochim. Biophys. Acta. 1491:303-308. https://doi.org/10.1016/S0167-4781(00)00046-4
  25. Pelleymounter, M. A., M. J. Cullen, M. B. Baker, R. Hecht, D. Winters, T. Boone and F. Collins. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Sci. 269:540-543. https://doi.org/10.1126/science.7624776
  26. Houseknecht, K. L., C. A. Baile, R. L. Matteri and M. E. Spurlock. 1998. The biology of leptin: a review. J. Anim. Sci. 76:1405-1420. https://doi.org/10.2527/1998.7651405x
  27. Scarpace, P. J., M. Matheny, B. H. Pollock and N. Tumer. 1997. Leptin increases uncoupling protein expression and energy expenditure. Am. J. Physiol. 273:E226-230.
  28. Taouis, M., J. W. Chen, C. Daviaud, J. Dupont, M. Derouet and J. Simon. 1998. Cloning the chicken leptin gene. Gene. 208:239-242. https://doi.org/10.1016/S0378-1119(97)00670-7
  29. Taouis, M., S. Dridi, S. Cassy, Y. Benomar, N. Raver, N. Rideau, M. Picard, J. Williams and A. Gertler. 2001. Chicken leptin: properties and actions. Domest. Anim. Endocrinol. 21:319-327. https://doi.org/10.1016/S0739-7240(01)00122-9
  30. Wang, M. Y., Y. Lee and R. H. Unger. 1999. Novel form of lipolysis induced by leptin. J. Biol. Chem. 274:17541-17544. https://doi.org/10.1074/jbc.274.25.17541
  31. Zhang, Y., R. Proenca, M. Maffei, M. Barone, L. Leopold and J. M. Friedman. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature. 372:425-432. https://doi.org/10.1038/372425a0

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