Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
권호 표지

The level of urinary malondialdehyde concentrations in vinyl chloride monomer exposure workers

염화비닐 노출 근로자의 요중 Malondialdehyde 농도 수준

  • Kim, Hyun Soo (Ilsan International Hospital, Dongguk University) ;
  • Kim, Chi-Nyon (Institute for Occupational Health, College of Medicine, Yonsei University) ;
  • Won, Jong Uk (Institute for Occupational Health, College of Medicine, Yonsei University) ;
  • Park, Jun Ho (Department of Preventive Medicine and Institute of Occupational Medicine, Wonju College of Medicine, Yonsei University) ;
  • Kim, Gwang Suk (College of Nursing, Yonsei University) ;
  • Ahn, Mi Hyun (Department of Nursing, Graduate school of Yonsei University) ;
  • Roh, Jaehoon (Institute for Occupational Health, College of Medicine, Yonsei University)
  • 김현수 (동국대학교 일산병원) ;
  • 김치년 (연세대학교 의과대학 산업보건연구소) ;
  • 원종욱 (연세대학교 의과대학 산업보건연구소) ;
  • 박준호 (연세대학교 원주의과대학 예방의학교실 및 직업의학연구소) ;
  • 김광숙 (연세대학교 간호대학) ;
  • 안미현 (연세대학교 대학원 간호학과) ;
  • 노재훈 (연세대학교 의과대학 산업보건연구소)
  • Received : 2006.11.24
  • Accepted : 2007.04.20
  • Published : 2007.06.30
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Abstract

The aim of this study was to investigate whether the exposure to vinyl chloride monomer(VCM) induces lipid peroxidation in workers by evaluating the concentration of malondialdehyde(MDA) in the urine in order to assess worker's oxidative stress due to exposure of vinyl chloride monomer. The subjects investigated in the study were divided into the experimental group; 18 workers exposed to VCM, and the control group; 19 workers unexposed to VCM. A gas chromatography/pulsed flame photometric detector(GC/PFPD) was utilized to analyze thiodiglycolic acid(TDGA), which was methylated with trimethylsilyldiazomethane (2.0M in diethyl ether) in urine and the urinary MDA, the product of lipid peroxidation, was determined by high-performance liquid chromatography/ultraviolet-visible detector after derivatized with 2,4-dinitrophenylhydrazine(DNPH). The concentrations of urinary TDGA in controls and VCM exposure workers were 0.13(2.01)mg/g Cr. GM(GSD) and 0.35(1.96)mg/gCr. GM(GSD), respectively. The concentrations of urinary MDA were $0.12(2.21){\mu}mol/gCr$. GM(GSD) in controls and $1.35(1.79){\mu}mol/gCr$. GM(GSD) in VCM exposure workers. As a result of simple regressions analysis between urinary concentration of TDGA and MDA in VCM exposure workers, it was found that the $R^2$ value was 0.261 (p=0.03) and the drinking and smoking did not affect their level. In conclusion, the workers exposed to VCM have a potentially to suffered by oxidative stress due to VCM exposure and the urinary MDA can be applicable to the marker of effect to assess the level of worker's VCM exposure.

Keywords

References

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