DOI QR코드

DOI QR Code

Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen

  • Kim, Hye-Jin (Haemaru Small Animal Referral Hospital) ;
  • Oh, Hyun-Ju (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Jang, Goo (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Min-Kyu (Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University)
  • Published : 20070300

Abstract

The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70ºC for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6% glycerol buffer and thawing at 70ºC for 8 sec with 5 × 107 spermatozoa. Even though the pregnancy rate (80%) and the whelping rate (24.5%) in the 5 × 106 spermatozoa inseminated group were lower than those of the 5 × 107 spermatozoa group, conception was confirmed with 5 × 106 spermatozoa. Although the pregnancy rate of intratubal insemination was low (20%) with 4 × 106 spermatozoa, this study is the first report to show the pregnancy rate of intratubal insemination with frozen-thawed ejaculated canine semen. In order to improve the pregnancy rate with intratubal insemination of canine spermatozoa, it is necessary to investigate the optimal insemination site of the uterine tube, the appropriate number of sperm, and the direct effect of buffer on oocytes.

Keywords

Acknowledgement

This study was financially supported by KOSEF (grant #M10625030005-06N250300510). The authors are grateful for a graduate fellowship provided by the Ministry of Education through the BK21 Program.

References

  1. Andersen K. Insemination with frozen dog semen based on a new insemination technique. Zuchthygiene 1975, 10, 1-4 https://doi.org/10.1111/j.1439-0531.1975.tb00286.x
  2. Cremonesi F, Salamon L, Groppetti D, Pecile A. Results of a single transcervical endoscopic insemination using frozen semen in the bitch. Vet Res Commun 2005, 29 (Suppl 2), 187-189 https://doi.org/10.1007/s11259-005-0039-8
  3. England GCW. Cryopreservation of dog semen: a review. J Reprod Fertil Suppl 1993, 47, 243-255
  4. Farstad W. Bitch fertility after natural mating and after artificial insemination with fresh or frozen semen. J Small Anim Pract 1984, 25, 561-565 https://doi.org/10.1111/j.1748-5827.1984.tb03429.x
  5. Farstad W, Berg KA. Factors influencing the success rate of artificial insemination with frozen semen in the dog. J Reprod Fertil Suppl 1989, 39, 289-292
  6. Ferguson JM, Renton JP, Farstad W, Douglas TA. Insemination of beagle bitches with frozen semen. J Reprod Fertil Suppl 1989, 39, 293-298
  7. Hammerstedt RH, Graham JK, Nolan JP. Cryopreservation of mammalian sperm: what we ask them to survive. J Androl 1990, 11, 73-88
  8. Holt WV. Basic aspects of frozen storage of semen. Anim Reprod Sci 2000, 62, 3-22 https://doi.org/10.1016/S0378-4320(00)00152-4
  9. Hori T, Ichikawa M, Kawakami E, Tsutsui T. Artificial insemination of frozen epididymal sperm in beagle dogs. J Vet Med Sci 2004, 66, 37-41 https://doi.org/10.1292/jvms.66.37
  10. Lee BC, Kim MK, Jang G, Oh HJ, Yuda F, Kim HJ, Hossein MS, Kim JJ, Kang SK, Schatten G, Hwang WS. Dogs cloned from adult somatic cells. Nature 2005, 436, 641 https://doi.org/10.1038/436641a
  11. Linde-Forsberg C, Forsberg M. Fertility in dogs in relation to semen quality and the time and site of insemination with fresh and frozen semen. J Reprod Fertil Suppl 1989, 39, 299- 310
  12. Linde-Forsberg C, Forsberg M. Results of 527 controlled artificial inseminations in dogs. J Reprod Fertil Suppl 1993, 47, 313-323
  13. Linde-Forsberg C, Ström Holst B, Govette G. Comparison of fertility data from vaginal vs intrauterine insemination of frozen-thawed dog semen: a retrospective study. Theriogenology 1999, 52, 11-23 https://doi.org/10.1016/S0093-691X(99)00106-5
  14. Morton DB, Bruce SG. Semen evaluation, cryopreservation and factors relevant to the use of frozen semen in dogs. J Reprod Fertil Suppl 1989, 39, 311-316
  15. Nothling JO, Gerstenberg C, Volkmann DH. Success with intravaginal insemination of frozen-thawed dog semen-a retrospective study. J S Afr Vet Assoc 1995, 66, 49-55
  16. Olar TT, Bowen RA, Pickett BW. Influence of extender, cryoperservative and seminal processing procedures on post thaw motility of canine spermatozoa frozen in straws. Theriogenology 1989, 31, 451-461 https://doi.org/10.1016/0093-691X(89)90550-5
  17. Pena A, Linde-Forsberg C. Effects of Equex, one- or twostep dilution, and two freezing and thawing rates on postthaw survival of dog spermatozoa. Theriogenology 2000, 54, 859-875 https://doi.org/10.1016/S0093-691X(00)00326-5
  18. Thomassen R, Farstad W, Krogenaes A, Fougner JA, Berg KA. Artificial insemination with frozen semen in the dog: a retrospective study. J Reprod Fertil Suppl 2001, 57, 341-346
  19. Tsutsui T, Kawakami E, Murao I, Oqasa A. Transport of spermatozoa in the reproductive tract of the bitch: observations through uterine fistula. Nippon Juigaku Zasshi 1989, 51, 560-565 https://doi.org/10.1292/jvms1939.51.560
  20. Tsutsui T, Hase M, Tanaka A, Fujimura N, Hori T, Kawakami E. Intrauterine and intravaginal insemination with frozen canine semen using an extender consisting of orvus ES paste-supplemented egg yolk tris-fructose citrate. J Vet Med Sci 2000, 62, 603-606 https://doi.org/10.1292/jvms.62.603
  21. Tsutsui T, Hori T, Yamada A, Kirihara N, Kawakami E. Intratubal insemination with fresh semen in dogs. J Vet Med Sci 2003, 65, 659-661 https://doi.org/10.1292/jvms.65.659
  22. Wilson MS. Non-surgical intrauterine artificial insemination in bitches using frozen semen. J Reprod Fertil Suppl 1993, 47, 307-311