Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Determination of pseudoephodrine, dextromethorphan and their metabolites in human urine by gas chromatography - mass spectrometry

GC/MS를 이용한 소변 중 Pseudoephedrine과 Dexrormethorphan 및 대사체의 동시분석

  • 이원웅 (경희대학교 약학대학 약학과) ;
  • 안성호 (경희대학교 약학대학 약학과) ;
  • 이성우 (경희대학교 약학대학 약학과) ;
  • 홍종기 (경희대학교 약학대학 약학과)
  • Received : 2007.05.28
  • Accepted : 2007.06.29
  • Published : 2007.08.25
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Abstract

This study has been described the metabolism and excretion in a healthy male urine collected for 24 hr after oral administration of a complex (pseudoephedrine and dextromethorphan). To detect the trace amounts of parent drugs and their metabolites, acid-hydrolyzed urine was extracted and derivatized with MSTFA and MBTFA followed by gas chromatography/mass spectrometric analysis. Two parent drugs and their metabolites were tentatively identified as their derivatives based on the mass spectral interpretation and compared with previous reports. In addition, the time profile of urinary excretion rate for parent drugs and metabolites was studied. On the basis of metabolites identified and excretion rate, the metabolic pathways of both drugs are suggested.

본 연구는 1명의 건강한 성인남자로부터 pseudoephedrine과 dextromethorphan 복합제제 약물의 복용 후 24시간동안 배설된 소변을 채취하여 이들 혼합약물의 대사과정과 배설에 관하여 연구를 수행 하였다. 소변 중에 약물과 대사체의 검출을 위하여 가수분해 및 추출과정을 거쳐 MSTFA와 MBTFA를 사용하여 유도체 반응 후 GC-MS로 동시 분석을 수행하였다. 각 대사체는 질량스펙트럼의 해석을 통하여 구조가 규명되었으며, 시간에 따른 모 약물과 대사체의 배설율을 조사하였으며, 본 실험결과로부터 pseudoephedrine과 dextromethorphan의 체내 대사경로를 제안하였다.

Keywords

References

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