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A Novel Mannose-binding Tuber Lectin from Typhonium divaricatum (L.) Decne (family Araceae) with Antiviral Activity Against HSV-II and Anti-proliferative Effect on Human Cancer Cell Lines

  • Luo, Yongting (College of Life Sciences, Sichuan University) ;
  • Xu, Xiaochao (College of Life Sciences, Sichuan University) ;
  • Liu, Jiwei (College of Life Sciences, Sichuan University) ;
  • Li, Jian (College of Life Sciences, Sichuan University) ;
  • Sun, Yisheng (College of Life Sciences, Sichuan University) ;
  • Liu, Zhen (College of Life Sciences, Sichuan University) ;
  • Liu, Jinzhi (College of Life Sciences, Sichuan University) ;
  • Damme, Els Van (Department of Molecular Biotechnology, Ghent University) ;
  • Balzarini, Jan (Rega Institute for Medical Research, Katholieke University Leuven) ;
  • Bao, Jinku (College of Life Sciences, Sichuan University)
  • 발행 : 2007.05.31

초록

A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 $\mu$g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 $\pm$ 6.8), Bre-04 (41.5 $\pm$ 4.8), and Lu-04 (11.4 $\pm$ 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with $TC_50$ and $EC_50$ of 5.176 mg/ml and 3.054 $\mu$g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.

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참고문헌

  1. Allen, A. K. (1995) Purification and characterization of an Nacetyllactosamine- specific lectin from tubers of Arum maculatum. Biochim. Biophys. Acta 1244, 129-132 https://doi.org/10.1016/0304-4165(94)00210-O
  2. Andrews, P. (1964) Estimation of molecular weights of proteins by Sephadex gel-filtration. Biochem. J. 91, 222-233 https://doi.org/10.1042/bj0910222
  3. Bains, J. S., Singh, T. J., Kamboj, S. S., Nijjar, K. K., Agrewal, J. N., Kumar, V., Kumar, A. and Saxena, A. K. (2005) Mitogenic and anti-proliferative activity of a lectin from the tubers of Voodoo lily (Sauromatum venosum). Biochim. Biophys. Acta 1723, 163-174 https://doi.org/10.1016/j.bbagen.2005.02.006
  4. Barre, A., Van Damme, E. J. M., Peumans, W. J. and Rouge, P. (1996) Structure-function relationship of monocot mannosebinding lectins. Plant Physiol. 112, 1531-1540 https://doi.org/10.1104/pp.112.4.1531
  5. Gaboriaud, C., Bissery, V., Benchetrit, T. and Mornon, J. P. (1987) Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences. FEBS Lett. 224, 149-155 https://doi.org/10.1016/0014-5793(87)80439-8
  6. Goldstein, I. J., Hufhes, R. C., Monsigny, M., Osawa, T. and Sharon, N. (1980) What should be called a lectin? Nature 285, 66
  7. Hirai, M., Nakamura, K., Imai, T. and Sato, T. (1993) cDNAs encoding for storage proteins in the tubers of taro (Colocasia esculenta Schott ). Jpn. J. Genet. 68, 229-236 https://doi.org/10.1266/jjg.68.229
  8. Karasaki, Y., Tsukamoto, S., Mizusaki, K., Sugiura, T. and Gotoh, S. (2001) A garlic lectin exerted an antitumor activity and induced apoptosis in human tumor cells. Food Res. Int. 34, 7-13 https://doi.org/10.1016/S0963-9969(00)00122-8
  9. Kaur, M., Singh, K., Rup, P. J., Saxena, A.K., Khan, R. H. Mohd, T. A., Sukhdev Singh Kamboj, S. S. and Singh, J. (2006) A tuber lectin from Arisaema helleborifolium Schott with antiinsect activity against melon fruit fly, Bactrocera cucurbitae (Coquillett) and anti-cancer effect on human cancer cell lines. Arch. Biochem. Biophys. 445, 156-165 https://doi.org/10.1016/j.abb.2005.10.021
  10. Kodama, E., Shigeta, S., Suzuki, T. and De Clercq, E. (1996) Application of a gastric cancer cell line (MKN-28) for antiadenovirus screening using the MTT method. Antiviral Res. 31, 159-164 https://doi.org/10.1016/0166-3542(96)06966-5
  11. Kong, W. W., Deng, Z. X., Fei, J., Wang, Q., Sun, X. F. and Tang, K. X. (2006) Characterization of a mannose-binding lectin gene from Typhonium divaricatum (L.) Decne. Afr. J. Biotechnol. 5, 793-799
  12. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 https://doi.org/10.1038/227680a0
  13. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265-275
  14. Marchetti, M., Mastromarino, P., Rieti, S., Seganti, L. and Orsi, N. (1995) Inhibition of herpes simplex, rabies and rubella viruses by lectins with defferent specificities. Res. Virol. 146, 211-215 https://doi.org/10.1016/0923-2516(96)80581-4
  15. Mo, H. Q., Rice, K. G., Evers, D. L., Winter, H. C., Peumans, W. J., Van Damme, E. J. M. and Goldstein, I. J. (1999) Xanthosoma sagittifolium tubers contain a lectin with two different types of carbohydrate-binding sites. J. Biol. Chem. 274, 33300-33305 https://doi.org/10.1074/jbc.274.47.33300
  16. Monks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Langley, J., Cronise, P. and Vaigro-Wolff, A. (1991) Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J. Natl. Cancer Inst. 83, 757-766 https://doi.org/10.1093/jnci/83.11.757
  17. Oda Y. and Minami K. (1986), Isolation and characterization of a lectin from tulip bulbs, Tulipa gesneriana. Eur. J. Biochem. 159, 239-245 https://doi.org/10.1111/j.1432-1033.1986.tb09859.x
  18. Okada, Y. and Kim, J. (1972) Incubation of concanavalin A with enveloped viruses and host cells. Virology 50, 507-515 https://doi.org/10.1016/0042-6822(72)90401-1
  19. Sandu, R. S., Arora, J. S., Chopra, S. K., Pelia, S. S., Kamboj, S. S., Naidu, Y. C. and Nath, I. (1990) Occurrence and isolation of new lectins of plants, fungi, and bacteria; in Lectins, Biology, Biochemistry, Clinical Biochemistry, Kocourek, J. and Freed, D. L. J. (eds.), pp. 19-33, Sigma Chem. Co., USA
  20. Shangary, S., Singh, J., Kamboj, S. S., Kamboj, K. K. and Sandhu, R. S. (1995) Purification and properties of four monocot lectins from the family araceae. Phytochemistry 40, 449-445 https://doi.org/10.1016/0031-9422(95)00229-Z
  21. Sharon, N. and Lis, H. (1989) Lectins as cell recognition molecules. Science 246, 227-234 https://doi.org/10.1126/science.2552581
  22. Singh, J., Singh, J. and Kamboj, S. S. (2004) A novel mitogenic and antiproliferative lectin from a wild cobra lily, Arisaema flavum. Biochem. Biophys. Res. Commun. 318, 1057-1065 https://doi.org/10.1016/j.bbrc.2004.04.135
  23. Van Damme, E. J. M., Goldstein, I. J., Vercammen, G., Vuylsteke, J. and Peumans, W. J. (1992) Lectins of members of the Amaryllidaceae are encoded by multigene families which show extensive homologies. Physiol. Plantarum. 86, 245-252 https://doi.org/10.1034/j.1399-3054.1992.860209.x
  24. Van Damme, E. J. M., Goossens, K., Smeets, K., Van Leuven, F., Verhaert, P. and Peumans, W. J. (1995a) The major tuber storage protein of araceae species is a lectin. Characterization and molecular cloning of the lectin from Arum maculatum. Plant Physiol. 107, 1147-1158 https://doi.org/10.1104/pp.107.4.1147
  25. Van Damme, E. J. M., Smeets, K. and Peumans, W. J. (1995b) The mannose binding monocot lectins and their genes; in: Lectins, BioMedical Perspectives, Pusztai, A. J., Bardocz, S. (eds.), pp. 59-80, Taylor and Francis Ltd, London, U.K.
  26. Van Damme, E. J. M., Peumans, W. J., Barre, A. and Rouge, P. (1998) Plant lectins: A composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles. Crit. Rev. Plant Sci. 17, 575-692 https://doi.org/10.1080/07352689891304276
  27. Von Heijine, G. (1986) A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14, 4683-4690 https://doi.org/10.1093/nar/14.11.4683
  28. Wang, H., Gao, J. and Ng, T. B. (2000) A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem. Biophys. Res. Commun. 275, 810-816 https://doi.org/10.1006/bbrc.2000.3373
  29. Wrigh, L. M., Reynolds, C. D., Rizkallah, P. J., Allen, A. K., Van Damme, E. J. M., Donovan, M. J. and Peumans, W. J. (1999) Structural characterisation of the native fetuin-binding protein Scilla campanulata agglutinin: a novel two-domain lectin. FEBS Lett. 468, 19-22 https://doi.org/10.1016/S0014-5793(00)01109-1

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  7. Bioinformatics analyses of the mannose-binding lectins from Polygonatum cyrtonema, Ophiopogon japonicus and Liparis noversa with antiproliferative and apoptosis-inducing activities vol.16, pp.6-7, 2009, https://doi.org/10.1016/j.phymed.2008.12.010
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  17. Clematis montana lectin, a novel mannose-binding lectin from traditional Chinese medicine with antiviral and apoptosis-inducing activities vol.30, pp.10, 2009, https://doi.org/10.1016/j.peptides.2009.06.027
  18. Isolation and characterization of a lectin with potentially exploitable activities from caper (Capparis spinosa) seeds vol.29, pp.5, 2009, https://doi.org/10.1042/BSR20080110
  19. Glycosylation of dengue virus glycoproteins and their interactions with carbohydrate receptors: possible targets for antiviral therapy vol.161, pp.7, 2016, https://doi.org/10.1007/s00705-016-2855-2
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  25. Molecular mechanisms ofLycoris aureaagglutinin-induced apoptosis and G2/M cell cycle arrest in human lung adenocarcinoma A549 cells, bothin vitroandin vivo vol.46, pp.3, 2013, https://doi.org/10.1111/cpr.12034
  26. In silico identification of novel hevein-like peptide precursors vol.38, pp.1, 2012, https://doi.org/10.1016/j.peptides.2012.07.025
  27. In vitro Antiviral Activity of Crinum latifolium Lectin Against Poxvirus Replication vol.8, pp.7, 2008, https://doi.org/10.3923/jbs.2008.1236.1240
  28. Antiproliferation and apoptosis of human tumor cell lines by a lectin (AMML) of Astragalus mongholicus vol.16, pp.6-7, 2009, https://doi.org/10.1016/j.phymed.2008.12.024
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