Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Application of a solid-phase fluorescence immunoassay to determine streptomycin residues in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major)

  • Chung, Hee Sik (Hapcheon Country Office) ;
  • Kim, Suk (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Chun, Myung Sun (College of Veterinary Medicine, Seoul National University) ;
  • Jung, Won Chul (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Dong Hyeok (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Shon, Ho Yeong (Agricultural Technology Center, Yangsan City Hall) ;
  • Lim, Jeong Ju (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • Accepted : 2007.05.08
  • Published : 2007.06.30
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Abstract

Parallux (IDEXX Laboratories, USA), a solid-phase fluorescence immunoassay (SPFIA) developed for antibiotics residue detection in milk, was applied for analysis of antibiotics in muscle tissue of olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major). Fishes were dipped in streptomycin 20 g/ton water, the recommended therapeutic dose, for 3 consecutive days. Muscle was sampled after 1st, 2nd, 3rd, 4th and 5th day drug treatment. The concentration of streptomycin in muscle, determined by SPFIA, was compared with that of internal standard (200 ppb as streptomycin). The absorbance ratio of sample to internal standard (S/C) was employed as an index to determine the muscle residues in fishes. The standard solutions were added to muscle samples to give final concentrations in muscle of 0.2 and 0.5 mg/ml to investigate the recovery rate. The recovery rates of all spiked samples were > 84% of the spiked value. Streptomycin was detected in muscles of fishes treated after the 1st day of withdrawal period. The present study showed that the SPFIA can be easily adopted in predicting muscle tissue residues for streptomycin in farmed fishes.

Keywords

References

  1. Baxter GA, Ferguson JP, O'Connor MC, Elliott CT. Detection of streptomycin residues in whole milk using an optical immunobiosensor. J Agric Food Chem 2001, 49, 3204-3207 https://doi.org/10.1021/jf001484l
  2. Bogialli S, Curini R, Corcia A, Lagana A, Mele M, Nazzari M. Simple confirmatory assay for analyzing residues of aminoglycoside antibiotics in bovine milk: hot water extraction followed by liquid chromatographytandem mass spectrometry. J Chromatogr A 2005, 1067, 93-100 https://doi.org/10.1016/j.chroma.2004.10.033
  3. Colorni A, Avtalion R, Knibb W, Berger E, Colorni B, Timan B. Histopathology of sea bass (Dicentrarchus labrax) experimentally infected with Mycobacterium marinum and treated with streptomycin and garlic (Allium sativum) extract. Aquaculture 1998, 160, 1-17 https://doi.org/10.1016/S0044-8486(97)00234-2
  4. Edder P, Cominoli A, Corvi C. Determination of streptomycin residues in food by solid-phase extraction and liquid chromatography with post-column derivatization and fluorometric detection. J Chromatogr A 1999, 830, 345-351 https://doi.org/10.1016/S0021-9673(98)00917-0
  5. FAO/WHO Expert Committee on Food Additives and World Health Organization. Evaluation of certain veterinary drug residues in food. Forty-eighth report of the joint FAO/WHO Expert Committee on Food Additives. World Health Organ Tech Rep Ser 1998, 879, 1-73
  6. Food and Drug Administration. Streptomycin residues in cattle tissues; Withdrawal of compliance policy guide. Fed Regist 2006, 71, 38650-38651
  7. Friedlander LG, Stephany RW. Dihydrostreptomycin and streptomycin. FAO Food Nutr Pap 2001, 41/14, 37-41
  8. Haasnoot W, Stouten P, Cazemier G, Lommen A, Nouws JFM, Keukens HJ. Immunochemical detection of aminoglycosides in milk and kidney. Analyst 1999, 124, 301-305 https://doi.org/10.1039/a807846g
  9. Habrda J, Malikova M. Persistance of streptomycin residues in the calf organism after parenteral therapeutic administration. Vet Med (Praha) 1976, 21, 167-174
  10. Horie M, Yoshida T, Kikuchi Y, Nakazawa H. Determination of streptomycin and dihydrostreptomycin in meat by liquid chromatography/mass spectrometry. Shokuhin Eiseigaku Zasshi 2001, 42, 374-378 https://doi.org/10.3358/shokueishi.42.374
  11. Ian C. TB or not TB? Pract Fishkeeping 1993, 7, 21-25
  12. Jung WC, Ha JY, Chung HS, Heo SH, Kim S, Lee HJ. Application of a solid-phase fluorescence immunoassay to determine ampicillin residues in muscle tissue of olive flounder (Paralichthy olivaceus). Korean J Vet Res 2006, 46, 291-294
  13. Kim S, Chung HS, Ha JY, Jung WC, Heo SH, Lee HJ. Application of a solid-phase fluorescence immunoassay to determine oxytetracycline and tetracycline residues in tissue of olive flounder (Paralichthys olivaceus). J Vet Med Sci 2006, 68, 1243-1245 https://doi.org/10.1292/jvms.68.1243
  14. Lee HJ, Lee MH, Han IK. Application of ELISA for the detection of penicillin antibiotic residues in live animal. Asian-Aust J Anim Sci 2000, 13, 1604-1608 https://doi.org/10.5713/ajas.2000.1604
  15. Lee HJ, Ryu PD, Lee H, Cho MH, Lee MH. Screening for penicillin plasma residues in cattle by enzyme-linked immunosorbent assay. Acta Vet Brno 2001, 70, 353-358 https://doi.org/10.2754/avb200170030353
  16. Mitchell JM, Griffiths MW, McEwen SA, McNab WB, Yee AJ. Antimicrobial drug residues in milk and meat: causes, concerns, prevalence, regulations, tests, and test performance. J Food Prot 1998, 6l, 742-756 https://doi.org/10.4315/0362-028X-61.6.742
  17. Mitic SS, Sunaric SM, Tosic SB. Determination of streptomycin in a pharmaceutical sample based on its degradation by hydrogen peroxide in the presence of copper (II). Anal Sci 2006, 22, 753-756 https://doi.org/10.2116/analsci.22.753
  18. Okerman L, De Wasch K, Van Hoof J. Detection of antibiotics in muscle tissue with microbiological inhibition tests: effects of the matrix. Analyst 1998, 123, 2361-2365 https://doi.org/10.1039/a804903c
  19. Okerman L, Wasch KD, Hoof JV. Simultaneous determination of different antibiotic residues in bovine and in porcine kidneys by solid-phase fluorescence immunoassay. J AOAC Int 2003, 86, 236-240
  20. Pezzella C, Ricci A, DiGiannatale E, Luzzi I, Carattoli A. Tetracycline and streptomycin resistance genes, transposons, and plasmids in Salmonella enterica isolates from animals in Italy. Antimicrob Agents Chemother 2004, 48, 903-908 https://doi.org/10.1128/AAC.48.3.903-908.2004
  21. Sekiguchi J, Miyoshi-Akiyama T, Augustynowicz-Kopec E, Zwolska Z, Kirikae F, Toyota E, Kobayashi I, Morita K, Kudo K, Kato S, uratsuji T, Mori T, Kirikae T. Detection of multidrug resistance in Mycobacterium tuberculosis. J Clin Microbiol 2007, 45, 179-192 https://doi.org/10.1128/JCM.00750-06
  22. Szekacs A. Development of enzyme-linked immunosorbent assay (ELISA) systems for environmental monitoring. Acta Biol Hung 1994, 45, 77-80
  23. U.S. Environmental Protection Agency. Fact Sheet Number 186 Streptomycin. U.S. Environmental Protection Agency, Washington, 1988
  24. van Bruijnsvoort M, Ottink SJM, Jonker KM, de Boer E. Determination of streptomycin and dihydrostreptomycin in milk and honey by liquid chromatography with tandem mass spectrometry. J Chromatogr A 2004, 1058, 137-142 https://doi.org/10.1016/j.chroma.2004.07.101
  25. Wilson DJ, Norman BB, Franti CE. Detection of antibiotic and sulfonamide residues in bob veal calf tissues: 967 cases (1987-1988). J Am Vet Med Assoc 1991, 199, 759-766