Asian-Australasian Journal of Animal Sciences

  • 제20권4호
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  • Pages.461-465
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  • 2007
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Insertional Variations of Two Porcine Endogenous Retroviruses (PERVs) in Korean Native Pigs and Asian Wild Boars

  • Jung, K.C. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Yu, S.L. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Kim, T.H. (Animal Genomics and Bioinformatics Division, National Livestock Research Institute) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Rogel-Gaillard, C. (Laboratoire de Radiobiologie et d’Etude du Genome) ;
  • Park, C.S. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Jin, D.I. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs Chungnam National University) ;
  • Moran, C. (Centre for Advanced Technologies in Animal Genetics and Reproduction, Faculty of Veterinary Science, University of Sydney) ;
  • Lee, J.H. (Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs Chungnam National University)
  • 투고 : 2006.05.03
  • 심사 : 2006.08.22
  • 발행 : 2007.04.01
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초록

Porcine Endogenous Retroviruses (PERVs) are a major concern in relation to xenotransplantation. Previous research indicated that PERVs are present at about 50 copies in the pig genome and their chromosomal insertion sites are different among pig breeds. We examined nine Korean native pigs and seven Asian Wild Boars for the presence of a PERV-A at SSC 1q2.4 and a PERV-B at SSC 7p1.1-2 previously reported in a Large White pig. The PERV-B at locus 7p1.1-2 displayed insertional variability in Korean native pigs and Asian Wild Boars. Using the primers for the PERV-A at 1q2.4 from Large White pig, we only can amplify an unclassified 798 bp sequence, which showed insertional variability only in Korean native pigs. This study indicates that there are differences within and between Asian and European pigs in PERV insertions and suggests that selection could generate PERV-free lines of pigs more suitable for xenotransplantation.

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참고문헌

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피인용 문헌

  1. Porcine Endogenous Retroviruses in Xenotransplantation—Molecular Aspects vol.6, pp.5, 2014, https://doi.org/10.3390/v6052062
  2. Investigation of Deletion Variation and Methylation Patterns in the 5' LTR of Porcine Endogenous Retroviruses vol.21, pp.11, 2008, https://doi.org/10.5713/ajas.2008.80065