Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effect of a Mixture of Conjugated Linoleic Acid (CLA) Isomers on T Cell Subpopulation and Responsiveness to Mitogen in Splenocytes of Male Broiler Chicks

  • Takahashi, Kazuaki (Graduate School of Agricultural Science, Tohoku University) ;
  • Kawamata, Kenji (Graduate School of Agricultural Science, Tohoku University) ;
  • Akiba, Yukio (Graduate School of Agricultural Science, Tohoku University)
  • Received : 2006.09.26
  • Accepted : 2007.01.18
  • Published : 2007.06.01
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Abstract

The experiments were conducted to determine effects of a mixture of conjugated linoleic acid (CLA) isomers on T cell subpopulations and responsiveness to mitogen of splenocytes in male broiler chicks. In experiment 1, birds (8-d old) were fed basal, CLA-(CLA) and safflower oil-supplemented (SA) diets which were formulated by supplementary 10 g CLA or safflower oil/kg to the basal diet for 14 d. Broiler starter diet, which mainly consisted of corn and soybean meal, was served as the basal diet. Proliferative response and interleukin (IL)-2-like activity stimulated by concanavalin (Con) A at a concentration of $10{\mu}g/ml$ of splenocytes in chicks fed the CLA diet were greater than in chicks fed the SA diet, but not at $20{\mu}g$ Con A/ml. Percentage of CD3-positive T cells in splenocytes did not differ between chicks fed the SA diet and CLA. Ratio of CD4-positive T cells to CD8- positive T cells was significantly affected by dietary fat source. In experiment 2, broiler chicks (1-d old) were fed the same diets as in experiment 1 for 14 d. Results of splenocyte proliferation to Con A were similar to those in experiment 1, but phytohemaggulutinin (PHA)- or pokeweed mitogen (PWM)- induced splenocyte proliferation did not differ between the CLA and SA fed groups. Supplementation with SA or CLA to the basal diet tended to have a depressive effect on the proliferation, with the greater effect being that of SA. In experiment 3, effect of an addition of CLA to splenocyte culture medium on splenocyte proliferation was determined. An addition of CLA to the culture medium resulted in reduction of the splenocyte proliferation to Con A, but an addition of linoleic acid. When PWM and PHA were used as mitogen, the inhibitory effect of CLA and linoleic acid on the proliferation did not differ. The results suggested that the effect of dietary CLA on splenocyte proliferation was similar to that of SA, although the effect of dietary CLA on sub-populations was slightly different from that of dietary SA. Further studies are needed to clarify whether use of CLA would be beneficial for maintaining or enhancing T cell immunity in chicks.

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References

  1. Akiba, Y. and T. Mastumoto. 1978. Effect of force-feeding and dietary cellulose on liver lipid accumulation and lipid composition of liver and plasma in growing chicks. J. Nutr. 108:739-748. https://doi.org/10.1093/jn/108.5.739
  2. Albers, R., R. P. J. van der Wielen, E. J. Brink, H. F. J. Hendriks, V. N. Dorovska-Taran and I. C. M. Mohede. 2003. Effects of cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid (CLA) isomers on immune function in healthy men. Eur. J. Clin. Nutr. 57:595-603. https://doi.org/10.1038/sj.ejcn.1601585
  3. Bassaganya-Riera, J., R. Hontecillas-Magarzo, K. Bregendahl, M. J. Wannemuehler and D. R. Zimmerman. 2001. Effects of dietary conjugated linoleic acid in nursery pigs of dirty and clean environments on growth, empty body composition, and immune competence. J. Anim. Sci. 79:714-721. https://doi.org/10.2527/2001.793714x
  4. Bassaganya-Riera, J., R. Hontecillas, D. R. Zimmerman and M. J. Wannemuehler. 2002. Long-term influence of lipid nutrition on the induction of CD8(+) responses to viral or bacterial antigens. Vaccine 20:1435-1444. https://doi.org/10.1016/S0264-410X(01)00465-0
  5. Bassaganya-Riera, J., R. M. Pogranichniy, S. C. Jobgen, P. G. Halbur, K. J. Yoon, M. O'Shea and R. Hontecillas. 2003. Conjugated linoleic acid ameliorates viral infectivity in a pig model of virally induced immunosuppression. J. Nutr. 133:3204-3214. https://doi.org/10.1093/jn/133.10.3204
  6. Calder, P. C. 1996. Effects of fatty acids and dietary lipids on the immune system. Proc. Nutr. Soc. 55:127-150. https://doi.org/10.1079/PNS19960015
  7. Chan, M. M., C. L. Chen, L. L. Ager and M. D. Cooper. 1988. Identification of the avian homologues of mammalian CD4 and CD8 antigens. J. Immunol. 140:2133-2138.
  8. Changhua, L., Y. Jindong, L. Defa, Z. Lidan, Q. Shiyan and X. Jianjun. 2005. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide. J. Nutr. 135:239-244. https://doi.org/10.1093/jn/135.2.239
  9. Chew, B. P., T. S. Wong, T. D. Shultz and N. S. Magnuson. 1997. Effects of conjugated dienoic derivatives of linoleic acid and beta-carotene in modulating lymphocyte and macrophage function. Anticancer Res. 17:1099-1106. https://doi.org/10.1097/01.cad.0000231482.15277.48
  10. Cook, M. E. 1999. Nutritional effects on vaccination. Adv. Vet. Med. 41:53-59. https://doi.org/10.1016/S0065-3519(99)80008-1
  11. Cook, M. E., C. C. Miller, Y. Park and M. W. Pariza. 1993. Immune modulation by altered nutrient metabolism: Nutritional control of immune-induced growth depression. Poult. Sci. 72:1301-1305. https://doi.org/10.3382/ps.0721301
  12. Du, M. and D. U. Ahn.. 2002. Effect of dietary conjugated linoleic acid on the growth rate of live birds and on the abdominal fat content and quality of broiler meat. Poult. Sci. 81:428-433. https://doi.org/10.1093/ps/81.3.428
  13. Hayek, M. G., S. N. Han, D. Y. Wu, B. A. Watkins, M. Meydani, J. L. Dorsey, D. E. Smith and S. N. Meydani. 1999. Dietary conjugated linoleic acid influences the immune response of young and old C57BL/6NCrlBR mice. J. Nutr. 129:32-38. https://doi.org/10.1093/jn/129.1.32
  14. Kelley, D. S., V. A. Simon, P. C. Taylor, I. L. Rudolph, P. G. Benito, J. Nelson, B. E. Mackey and K. L. Erickson. 2001. Dietary supplementation with conjugated linoleic acid increased its concentration in human peripheral blood mononuclear cells, but did not alter their function. Lipids 36:669-674. https://doi.org/10.1007/s11745-001-0771-z
  15. Jang, I. S., H. Y. Yang and Y. H. Ko. 2004. Effect of conjugated linoleic acid on intestinal and hepatic antioxidant enzyme activity and lipid peroxidation in broiler chickens. Asian-Aust. J. Anim. Sci. 17:1162-1167. https://doi.org/10.5713/ajas.2004.1162
  16. Kelley, D. S., P. C. Taylor, I. L. Rudolph, P. G. Benito, J. Nelson, B. E. Mackey and K. L. Erickson. 2000. Dietary conjugated linoleic acid did not alter immune status in young healthy women. Lipids 35:1065-1071. https://doi.org/10.1007/s11745-000-0620-0
  17. Kelley, D. S., J. M. Warren, V. A. Simon, G. Bartolini, B. E. Mackey and K. L. Erickson. 2002. Similar effects of c9,t11-CLA and t10,c12-CLA on immune cell functions in mice. Lipids 37:725-728. https://doi.org/10.1007/s11745-002-0954-7
  18. Miller, C. C., Y. Park, M. W. Pariza and M. E. Cook. 1994. Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochem. Biophy. Res. Comm. 198:1107-1112. https://doi.org/10.1006/bbrc.1994.1157
  19. Myers, T. J., H. S. Lillehoj and R. H. Fetter. 1992. Partial purification and characterization of chicken interleukin-2 activity. Vet. Immunol. Immunopath. 34:97-114. https://doi.org/10.1016/0165-2427(92)90154-I
  20. Nugent, A. P., H. M. Roche, E. J. Noone, A. Long, D. K. Kelleher and M. J. Gibney. 2005. The effects of conjugated linoleic acid supplementation on immune function in healthy volunteers. Eur. J. Clin. Nutr. 59:742-750. https://doi.org/10.1038/sj.ejcn.1602132
  21. O'Shea, M., J. Bassaganya-Riera and I. C. M. Mohede. 2004. Immunomodulatory properties of conjugated linoleic acid. Amer. J. Clin. Nutr. 79:1199S-1206S. https://doi.org/10.1093/ajcn/79.6.1199S
  22. SAS Iinstitute. 1988. SAS/STAT User's Guide: Statistics, Version 6.03. SAS Institute, Inc., Cray, NC, USA.
  23. Sugano, M., A. Tsujita, M. Yamasaki, M. Noguchi and K. Yamada. 1998. Conjugated linoleic acid modulates tissue levels of chemical mediators and immunoglobulins in rats. Lipids 33:521-527. https://doi.org/10.1007/s11745-998-0236-4
  24. Szymczyk, B., P. Pisulewski, W. Szczurek and P. Hanczakowski. 2001. Effects of conjugated linoleic acid on growth performance, feed conversion efficiency, and subsequent carcass quality in broiler chickens. Br. J. Nutr. 85:465-473. https://doi.org/10.1079/BJN2000293
  25. Song, H. J., I. Grant, D. Rotondo, I. Mohede, N. Sattar, S. D. Heys and K. W. P. Wahle. 2005. Effect of CLA supplementation on immune function in young healthy volunteers. Eur. J. Clin. Nutr. 59:508-517. https://doi.org/10.1038/sj.ejcn.1602102
  26. Takahashi, K. and Y. Akiba. 1996. Effect of dietary corticosterone on mononuclear cells proliferation to mytogen in spleen and thymus of broiler chickes. Anim. Sci. Tech. (Japan) 67:160-164.
  27. Takahashi, K., K. Kawamata, Y. Akiba, T. Iwata and M. Kasai. 2002a. Influence of dietary conjugated linoleic acid isomers on early inflammatory responses in male broiler chickens. Br. Poult. Sci. 43,47-53.
  28. Takahashi, K., K. Kawamata, Y. Akiba, T. Iwata and M. Kasai. 2002b. Dietary conjugated linoleic acids alleviates early inflammatory response caused by lipopolysaccharide injection in male broiler chicks. Anim. Sci. J. 73:41-46. https://doi.org/10.1046/j.1344-3941.2002.00006.x
  29. Takahashi, K., K. Kawamata, Y. Akiba, T. Iwata and M. Kasai. 2003. Effect of a mixture of conjugated linoleic acid isomers on growth performance and antibody production in broiler chicks. Br. J. Nutr. 89:691-694. https://doi.org/10.1079/BJN2003822
  30. Wong, M. W., B. P. Chew, T. S. Wong, H. L. Hosick, T. D. Boylston and T. D. Shultz. 1997. Effects of dietary conjugated linoleic acid on lymphocyte function and growth of mammary tumors in mice. Anticancer Res. 17:987-993.
  31. Yamasaki, M., K. Kishihara, K. Mansho, Y. Ogino, M. Kasai, M. Sugano, H. Tachibana and K. Yamada. 2000. Dietary conjugated linoleic acid increases immunoglobulin productivity of Sprague-Dawley rat spleen lymphocytes. Biosci. Biotech. Biochem. 64:2159-2164. https://doi.org/10.1271/bbb.64.2159
  32. Yamasaki, M., H. Chujo, A. Hirao, N. Koyanagi, T. Okamoto, N. Tojo, A. Oishi, T. Iwata, Y. Yamauchi-Sato, T. Yamamoto, K. Tsutsumi, H. Tachibana and K. Yamada. 2003. Immunoglobulin and cytokine production from spleen lymphocytes is modulated in C57BL/6J mice by dietary cis-9, trans-11 and trans-10, cis-12 conjugated linoleic acid. J. Nutr. 133:784-788. https://doi.org/10.1093/jn/133.3.784
  33. Zhang, H., Y. Guo and J. Yuan. 2005. Conjugated linoleic acid enhanced the immune function in broiler chicks. Br. J. Nutr. 94:746-752. https://doi.org/10.1079/BJN20051482

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