Molecules and Cells

  • 제24권3호
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  • Pages.394-408
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  • 2007
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Combining In Silico Mapping and Arraying: an Approach to Identifying Common Candidate Genes for Submergence Tolerance and Resistance to Bacterial Leaf Blight in Rice

  • Kottapalli, Kameswara Rao (Plant Genome Research Unit, National Institute of Agrobiological Sciences) ;
  • Satoh, Kouji (Plant Genome Research Unit, National Institute of Agrobiological Sciences) ;
  • Rakwal, Randeep (Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST) West) ;
  • Shibato, Junko (Plant Genome Research Unit, National Institute of Agrobiological Sciences) ;
  • Doi, Koji (Plant Genome Research Unit, National Institute of Agrobiological Sciences) ;
  • Nagata, Toshifumi (Plant Genome Research Unit, National Institute of Agrobiological Sciences) ;
  • Kikuchi, Shoshi (Plant Genome Research Unit, National Institute of Agrobiological Sciences)
  • 투고 : 2007.06.07
  • 심사 : 2007.07.03
  • 발행 : 2007.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

Several genes/QTLs governing resistance/tolerance to abiotic and biotic stresses have been reported and mapped in rice. A QTL for submergence tolerance was found to be co-located with a major QTL for broad-spectrum bacterial leaf blight (bs-blb) resistance on the long arm of chromosome 5 in indica cultivars FR13A and IET8585. Using the Nipponbare (japonica) and 93-11 (indica) genome sequences, we identified, in silico, candidate genes in the chromosomal region [Kottapalli et al. (2006)]. Transcriptional profiling of FR13A and IET8585 using a rice 22K oligo array validated the above findings. Based on in silico analysis and arraying we observed that both cultivars respond to the above stresses through a common signaling system involving protein kinases, adenosine mono phosphate kinase, leucine rich repeat, PDZ/DHR/GLGF, and response regulator receiver protein. The combined approaches suggest that transcription factor EREBP on long arm of chromosome 5 regulates both submergence tolerance and blb resistance. Pyruvate decarboxylase and alcohol dehydrogenase, co-located in the same region, are candidate downstream genes for submergence tolerance at the seedling stage, and t-snare for bs-blb resistance. We also detected up-regulation of novel defense/stress-related genes including those encoding fumaryl aceto acetate (FAA) hydrolase, scramblase, and galactose oxidase, in response to the imposed stresses.

키워드

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