Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Treatment of Diabetic Mouse Wounds using Blood Bank Platelet Concentrates

혈액은행의 혈소판농축액을 이용한 당뇨생쥐의 창상치유

  • Han, Seung-Kyu (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Jeong, Seong Ho (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Lee, Byung Il (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Kim, Woo Kyung (Department of Plastic Surgery, Korea University College of Medicine) ;
  • Gottrup, Finn (The University Center of Wound Healing, Odense University Hospital)
  • 한승규 (고려대학교 의과대학 성형외과학교실) ;
  • 정성호 (고려대학교 의과대학 성형외과학교실) ;
  • 이병일 (고려대학교 의과대학 성형외과학교실) ;
  • 김우경 (고려대학교 의과대학 성형외과학교실) ;
  • Received : 2006.09.04
  • Published : 2007.05.10
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Abstract

Purpose: Many clinical trials have shown the effectiveness of platelet releasate on chronic wounds. However, a large volume of blood must be aspirated from a patient and a platelet separator is required. Here, we hypothesized that platelet concentrate obtained from a blood bank (PCBB) would be also effective at stimulating wound healing. The purpose of this study was to investigate the effectiveness of PCBB on accelerating healing of diabetic wounds in vivo. Methods: Round wounds of 5 mm diameter were made at four sites(two wounds on the left and two on the right side) on the backs of nine diabetic mice. Three hundred million platelets suspended in 0.05 ml fibrinogen were dispersed on each wound on left sides. Same amount of fibrinogen without platelets was dispersed on right side control wounds. Thereafter, 0.05 ml thrombin was applied to the each wound. Ten days after wound treatment, healed wounds were excised and the extent of wound healing in each group was compared. Results: Quantitative histologic analysis of epithelial gap distances revealed that PCBB treatment had greatly accelerated wound healing. Mean epithelial gap distances for PCBB treated and control wounds were $2.5{\times}0.6mm\;and\;3.6{\times}0.5mm$, respectively(p<0.05). Conclusion: Our results suggest that PCBB has potential to accelerate the healing of diabetic wounds.

Keywords

References

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