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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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DNA-dependent Protein Kinase Mediates V(D)J Recombination via RAG2 Phosphorylation

  • Hah, Young-Sool (Department of Biochemistry and MRCND, School of Medicine and Gyeongsang Institute of Health Sciences, Gyeongsang National University) ;
  • Lee, Jung-Hwa (Department of Biochemistry and MRCND, School of Medicine and Gyeongsang Institute of Health Sciences, Gyeongsang National University) ;
  • Kim, Deok-Ryong (Department of Biochemistry and MRCND, School of Medicine and Gyeongsang Institute of Health Sciences, Gyeongsang National University)
  • Published : 2007.05.31
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Abstract

V(D)J recombination, a site-specific gene rearrangement process occurring during the lymphocyte development, begins with DNA double strand breaks by two recombination activating gene products (RAG1/2) and finishes with the repair process by several proteins including DNA-dependent protein kinase (DNA-PK). In this report, we found that RAG2 was specifically phosphorylated by DNA-PK at the $365^{th}$ serine residue, and this phosphorylated RAG2 affected the V(D)J recombination activity in cells in the GFP expression-based assay. While the V(D)J recombination activity between wild-type RAG2 and mutant S365A RAG2 in the assay using a signal joint substrate was undistinguishable in DNA-PK deficient cells (M059J), the activity with wild-type RAG2 was largely increased in DNA-PK proficient cells (M059K) in comparison with mutant RAG2, suggesting that RAG2 phosphorylation by DNA-PK plays a crucial role in the signal joint formation during V(D)J recombination.

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References

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