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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Saxatilin, a Snake Venom Disintegrin, Suppresses TNF-α-induced Ovarian Cancer Cell Invasion

  • Kim, Dong-Seok (Department of Biochemistry, College of Science, Yonsei University) ;
  • Jang, Yoon-Jung (Department of Biochemistry, College of Science, Yonsei University) ;
  • Jeon, Ok-Hee (Department of Biochemistry, College of Science, Yonsei University) ;
  • Kim, Doo-Sik (Department of Biochemistry, College of Science, Yonsei University)
  • Published : 2007.03.31
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Abstract

Saxatilin is a disintegrin known to inhibit tumor progression in vivo and in vitro. The role of saxatilin in cancer cell invasion was examined by a modified Boyden chamber assay in MDAH 2774 human ovarian cancer cell line. Saxatilin (50 nM) significantly inhibited cancer cell invasion induced by tumor necrosis factor-$\alpha$ (TNF-a$\alpha$). Saxatilin also reduced MMP-9 mRNA levels in cancer cells in a dosedependent manner. In addition, TNF-$\alpha$-induced MMP-9 activity was reduced by the treatment of saxatilin. These results indicate that transcriptional regulation of MMP-9 is an important mechanism for the tumor suppressive effects of saxatilin in MDAH 2774 human ovarian cancer cells.

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References

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