Asian-Australasian Journal of Animal Sciences

  • 제20권6호
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  • Pages.821-826
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  • 2007
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effect of the Polymorphisms of Keratin Associated Protein 8.2 Gene on Fibre Traits in Inner Mongolia Cashmere Goats

  • Liu, Haiying (College of Animal Science and Technology, China Agricultural University) ;
  • Li, Ning (State Key Laboratory of Biotechnology, China Agricultural University) ;
  • Jia, Cunling (College of Animal Science and Technology, Northwest A & F University) ;
  • Zhu, Xiaoping (College of Animal Science and Technology, China Agricultural University) ;
  • Jia, Zhihai (College of Animal Science and Technology, China Agricultural University)
  • 투고 : 2006.10.12
  • 심사 : 2007.01.04
  • 발행 : 2007.06.01
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초록

The aim of the experiment was to detect polymorphisms in the keratin-associated protein 8.2 (KAP8.2) gene to determine associations between the genotype and fibre traits in Chinese Inner Mongolia cashmere goats. The fibre traits data investigated were cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length. Five hundred and forty-two animals were used to detect polymorphisms in the complete coding sequence of the hircine KAP8.2 gene by means of PCR-SSCP. The results identified six genotypes, AA, BB, DD, AB, AD and BD, coded for by three different alleles A, B and D. Two SNPs in the coding region were confirmed by sequencing, which were A214G and T218C respectively. The relationships between the genotypes and cashmere fibre diameter, combed cashmere weight, cashmere fibre length and guard hair length were analyzed. There were significant differences (p<0.01) between the associations of the different genotypes with cashmere fibre diameter, cashmere weight and hair length. Cashmere length was the only trait that was not associated with the genotypes. The genotype AA (0.73) was found to be predominant in Inner Mongolia cashmere goats and the animals with this genotype had the thinnest cashmere fibre diameter compared with the other genotypes. These results suggested that polymorphisms in the hircine KAP8.2 gene may be a potential molecular marker for cashmere fibre diameter in cashmere goats.

키워드

참고문헌

  1. Adelson, D. L., D. E. Hollis and G. H. Brown. 2002. Wool fibre diameter and follicle density are not specified simultaneously during wool follicle initiation. Aust. J. Agric. Res. 53:1003-1009. https://doi.org/10.1071/AR01200
  2. Aoki, N., K. Ito and M. Ito. 1997. Isolation and characterization of mouse high-glycine/tyrosine proteins. J. Biol. Chem. 272:30512-30518. https://doi.org/10.1074/jbc.272.48.30512
  3. Arora, R. and S. Bhatia. 2006. Genetic Diversity of Magra Sheep from India Using Microsatellite Analysis. Asian-Aust. J. Anim. Sci. 19(7):938-942. https://doi.org/10.5713/ajas.2006.938
  4. Bai, J. Y., Q. Zhang, J. Q. Li, Er-Ji Dao and X. P. Jia. 2006. Estimates of genetic parameters and genetic trends for production traits of Inner Mongolian White Cashmere goat. Asian-Aust. J. Anim. Sci. 19(1):13-18.
  5. Bigham, M. L., C. A. Morris, B. R. Southey and R. L. Baker. 1993. Heritabilities and genetic correlations for live weights and fibre traits in New Zealand cashmere goats. Livest. Prod. Sci. 33:91-104. https://doi.org/10.1016/0301-6226(93)90241-9
  6. Bishop, S. C. and A. J. F. Russel. 1996. The inheritance of fibre traits in a crossbred population of cashmere goats. Anim. Sci. 63:429-436. https://doi.org/10.1017/S1357729800015320
  7. Bray, A. R., D. O'Connell and D. J. Saville. 2002. Genes with major effects on wool traits detected in Finn cross sheep. Proc. NZ. Soc. Anim. Prod. 62:65-68.
  8. Chen, S. L., M. H. Li, Y. J. Li, S. H. Zhao, C. Z. Yu, M. Yu, B. Fan and K. Li. 2001. RAPD Variation and Genetic Distances among Tibetan, Inner Mongolia and Liaoning Cashmere Goats. Asian-Aust. J. Anim. Sci. 14(11):1520-1522. https://doi.org/10.5713/ajas.2001.1520
  9. Jia C. L., N. Li, X. B. Zhao, X. P. Zhu and Z. H. Jia. 2005. Association of single nucleotide polymorphisms in exon 6 region of BMPIP gene with litter size traits in sheep. Asian-Aust. J. Anim. Sci. 18(10):1375-1378. https://doi.org/10.5713/ajas.2005.1375
  10. Fratini, A., B. C. Powell and G. E. Rogers. 1992. Sequence, expression, and evolutionary conservation of a gene encoding a glycine/tyrosine-rich keratin-associated protein of hair. J. Biol. Chem. 265:4511-4518.
  11. Frenkel, M. J., J. M. Gillespie and P. J. Reis. 1974. Factors influencing the biosynthesis of the tyrosine-rich proteins of wool. Aust. J. Biol. Sci. 27:31-38.
  12. Gillespie, J. M. 1990. The proteins of hair and other hard $\alpha$-keratins. In: Cellular and Molecular Biology of Intermediate Filaments (Ed. R. D. Goldman and P. M. Steinert). Plenum Press, New York. pp. 95-128.
  13. Henry, H. M., K. G. Dodds, T. Wuliji, Z. A. Jenkins, A. E. Beattie and G. W. Montgomery. 1998. A genome screen for QTL for wool traits in a Merino $\times$ Romney backcross flock. Wool Tech. 46:213-217.
  14. Kuczek, E. S. and G. E. Rogers. 1987. Sheep wool (glycine+tyrosine)-rich keratin genes: A family of low sequence homology. Eur. J. Biochem. 166:79-85. https://doi.org/10.1111/j.1432-1033.1987.tb13486.x
  15. McCarthy, B. J. 1998. Specialty animal fibres. Textiles. 1:6-8.
  16. McLaren, R., G. Rogers, K. Davies, J. Maddox and G. Montgomery. 1997. Linkage mapping of wool keratin and keratin-associated protein genes in sheep. Mamm. Genome. 8:938-940. https://doi.org/10.1007/s003359900616
  17. Parsons, Y. M., D. W. Cooper and L. R. Piper. 1994. Evidence of linkage between high-glycine-tyrosine keratin gene loci and wool fibre diameter in a Merino half-sib family. Anim. Genet. 25:105-108. https://doi.org/10.1111/j.1365-2052.1994.tb00414.x
  18. Parsons, Y. M., M. R. Fleet and D. W. Cooper. 1999. Isolation of the ovine agouti coding sequence. Pigment Cell Res. 12:394-397. https://doi.org/10.1111/j.1600-0749.1999.tb00524.x
  19. Pattie, W. A. and B. J. Restall. 1989. The inheritance of cashmere in Australian goats. 2. Genetic parameters and breeding values. Livest. Prod. Sci. 21:251-261. https://doi.org/10.1016/0301-6226(89)90054-7
  20. Rogers, G. R., J. G. H. Hickford and R. Bickerstaffe. 1994. Polymorphism in two genes for B2 high sulfur proteins of wool. Anim. Genet. 25:407-415. https://doi.org/10.1111/j.1365-2052.1994.tb00531.x
  21. Rogers, M. A., L. Langbein, H. Winter, C. Eemann, S. Praetzel and J. Schweizer. 2002. Characterization of a first domain of human high glycine-tyrosine and high sulfur keratin-associated protein (KAP) genes on chromosome 21q22.1. J. Biol. Chem. 277:48993-49002. https://doi.org/10.1074/jbc.M206422200
  22. SAS. 2001. User' Guide: Statistics. Version 8.2, Cary, NC, USA.
  23. Wood, N. J., S. H. Phua and A. M. Crawford. 1992. A dinucleotide repeat polymorphism at the glycine-rich and tyrosine-rich keratin locus in sheep. Anim. Genet. 23:391. https://doi.org/10.1111/j.1365-2052.1992.tb00164.x
  24. Zhou, H. M., D. Allain, J. Q. Li, W. G. Zhang and X. C. Yu. 2003. Effects of non-genetic factors on production traits of Inner Mongolia cashmere goats in China. Small Rumin. Res. 47:85-89. https://doi.org/10.1016/S0921-4488(02)00246-8

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