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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Cloning and Expression Analysis of a Novel Mouse Zinc Finger Protein Gene Znf313 Abundantly Expressed in Testis

  • Li, Na (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Sun, Huaqin (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Wu, Qiaqing (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Tao, Dachang (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Zhang, Sizhong (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University) ;
  • Ma, Yongxin (Department of Medical Genetics, West China Hospital, Division of Human Morbid Genomics, State Key Laboratory of Biotherapy of Human Diseases, Sichuan University)
  • Published : 2007.03.31
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Abstract

We have cloned a novel mouse zinc finger protein gene Znf313 by rapid amplification of cDNA ends (RACE) according to the homologue of human ZNF313 gene. The cDNA is 2,163 base pairs (bp) in length and encodes a 229 amino acids (aa) protein with a $C_3HC_4$ ring finger domain and three $C_2H_2$ domains. 89% and 93% nucleotide (nt) and aa sequence identity is observed with its human homologue. Revealed by Northern blot and RT-PCR, full mRNA consists of 2.16 kb and widely expresses in tissues as a single transcript, most abundantly in heart, liver, kidney and testis. The expression of Znf313 in testis is detected in all development stages. Western blot analysis also reveals that Znf313 is expressed in the tissues. Immunohistochemical staining and subcellular localization demonstrate that Znf313 is expressed both in the cytoplasm and nucleus whereas predominantly localized in the nucleus. Present data suggests that Znf313 gene might play a fundamental role in gene transcription and regulation in organism and relates to spermatogenesis.

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References

  1. Barlow, P. N., Luisi, B., Milner, A., Elliott, M. and Everett, R. (1994) Structure of the C3HC4 domain by $^{1}H$-nuclear magnetic resonance spectroscopy. A new structural class of zinc-finger. J. Mol. Biol. 237, 201-211. https://doi.org/10.1006/jmbi.1994.1222
  2. Borden, K. L. and Freemont, P. S. (1996) The RING finger domain: a recent example of a sequence-structure family. Curr. Opin. Struct. Biol. 63, 95-401.
  3. Cooke, H. J., Hargreave, T. and Elliott, D. J. (1998) Understanding the genes involved in spermatogenesis: A progress report. Ferti Steril. 69, 989-999. https://doi.org/10.1016/S0015-0282(98)00071-5
  4. Cunliffe, V., Koopman, P., McLaren, A. and Trowsdale, J. (1990) A mouse zinc finger gene which is transiently expressed during spermatogenesis. EMBO J. 9, 197-205.
  5. Free mont, P. S. (1993) The ring finger. A novel protein sequence motif related to the zinc finger. Ann. NY Acid Sci. 684, 174-192. https://doi.org/10.1111/j.1749-6632.1993.tb32280.x
  6. Frohman, M. A., Dush, M. K. and Martin, G. R. (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. https://doi.org/10.1073/pnas.85.23.8998
  7. Fujii, T., Tamura, K., Copeland, N. G., Gillbert, D. J., Jenkins, N. A., Yomogida, K. and Tanaka, H. (1999) Sperizin is a murine ring zinc-finger protein specifically expressed in haploid germ cells. Genomics 57, 94-101. https://doi.org/10.1006/geno.1998.5738
  8. Hammarstrom, A., Berndt, K. D., Sillard, R., Adermann, K. and Otting, G. (1996) Solution structure of a naturally-occurring zinc-peptide complex demonstrates that the N-terminal zincbinding module of the Lasp-1 LIM domain is an independent folding unit. Biochemistry 35, 12723-12732. https://doi.org/10.1021/bi961149j
  9. Hecht, N. B. (1995) The making of a spermatozoon: a molecular perspective. Dev. Genet. 16, 95-103. https://doi.org/10.1002/dvg.1020160202
  10. Jiao, K., Zhou, Y. and Hogan, B. L. (2002) Identification of mZNF8, a mouse Kruppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1. Mol. Cell. Biol. 22, 7633-7644. https://doi.org/10.1128/MCB.22.21.7633-7644.2002
  11. Klug, A. and Schwabe, J. W. (1995) Protein motifs 5.Zinc fingers. FASEB J. 9, 597-604. https://doi.org/10.1096/fasebj.9.8.7768350
  12. Li, J. Z., Li, J. and Fu, J. L. (2001) Zinc finger protein gene ZNF191. Life of Chemistry 21, 116-117.
  13. Ma, Y. X., Zhang, S. Z., Hou, Y. P. and Huang, X. L. (2003) Indentification of a novel human zinc finger protein gene ZNF313. Acta Bioch. Bioph. Sin. 35, 230-237.
  14. Palmer, M. S., Berta, P., Sinclair, A. H., Pym, B. and Goodfellow, P. N. (1990) Comparation of human ZFY and ZFX transcripts. Proc. Natl. Acid. Sci. USA 87, 1681-1685. https://doi.org/10.1073/pnas.87.5.1681
  15. Ragoussis, J., Senger, G., Mockridge, I., Sanseau, P., Ruddy, S., Dudley, K. and Sheer, D. (1992) A testis-expressed Zn finger gene (ZNF76) in human 6p21.3 centromeric to the MHC is closely linked to the human homolog of the t-complex gene tcp-11.Genomics 3, 673-679.
  16. Sassone-Corsi, P. (1997) Transcriptional checkpoints determining the fate of male germ cells. Cell 88, 163-166. https://doi.org/10.1016/S0092-8674(00)81834-6
  17. Vogt, P. H. (1998) Human chromosome deletions in Yq11, AZF candidate genes and male fertility: history and update. Mol. Hum. Reprod. 4, 739-744. https://doi.org/10.1093/molehr/4.8.739
  18. Willison, K. and Ashworth, A. (1987) Mammalian spermatogenic gene expression. Trends Genet. 3, 351-355. https://doi.org/10.1016/0168-9525(87)90296-4
  19. Zhou, C., Li, L. Y. and Lu, G. X. (2005) Molecular cloning and character analysis of the mouse zinc finger protein gene ZFP474 exclusively expressed in testis and ovary. Acta Genetica Sinica. 32, 155-162.

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