Asian-Australasian Journal of Animal Sciences

  • 제20권9호
  • /
  • Pages.1349-1353
  • /
  • 2007
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Expression Characterization, Polymorphism and Chromosomal Location of the Porcine Calsarcin-3 Gene

  • Wang, Heng (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agriculture Sciences) ;
  • Yang, Shulin (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agriculture Sciences) ;
  • Tang, Zhonglin (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agriculture Sciences) ;
  • Mu, Yulian (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agriculture Sciences) ;
  • Cui, Wentao (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agriculture Sciences) ;
  • Li, Kui (Department of Gene and Cell Engineering, Institute of Animal Science Chinese Academy of Agriculture Sciences)
  • 투고 : 2007.01.16
  • 심사 : 2007.04.07
  • 발행 : 2007.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Calcineurin is a calmodulin dependent protein that functions as a regulator of muscle cell growth and function. Agents capable of interacting with calcineurin could have important applications in muscle disease treatment as well as in the improvement of livestock production. Calsarcins comprise a family of muscle-specific calcineurin binding proteins which play an important role in modulating the function of calcineurin in muscle cells. Recently, we described the first two members of the calsarcin family (calsarcin-1 and calsarcin-2) in the pig. Here, we characterized the third member of the calsarcin family, calsarcin-3, which is also expressed specifically in skeletal muscle. However, unlike calsarcin-1 and calsarcin-2, the calsarcin-3 mRNA expression in skeletal muscle kept rising throughout the prenatal and postnatal development periods. In addition, radiation hybrid mapping indicated that porcine calsarcin-3 mapped to the distal end of the q arm of pig chromosome 2 (SSC2). A C/T single nucleotide polymorphism site in exon 5 was genotyped using the denaturing high performance liquid chromatography (DHPLC) method and the allele frequencies at this locus were significantly different among breeds.

키워드

참고문헌

  1. Chin, E. R., E. N. Olson, J. A. Richardson, Q. Yang, C. Humphries, J. M. Shelton, H. Wu, W. Zhu, R. Bassel-Duby and R. S. Williams. 1998. A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type. Genes Dev. 12:2499-2509 https://doi.org/10.1101/gad.12.16.2499
  2. Faulkner, G., A. Pallavicini, A. Comelli, M. Salamon, G. Bortoletto, C. Ievolella, S. Trevisan, S. Kojic, F. Dalla Vecchia, P. Laveder, G. Valle and G. Lanfranchi. 2000. FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle. J. Biol. Chem. 275:41234-41242 https://doi.org/10.1074/jbc.M007493200
  3. Fonseca, S., I. J. Wilsons, G. W. Horgan and C. A. Maltin. 2003. Slow fiber cluster pattern in pig longissimus thoracis muscle: implications for myogenesis. J. Anim. Sci. 81:973-983 https://doi.org/10.2527/2003.814973x
  4. Frey, N., J. A. Richardson and E. N. Olson. 2000. Calsarcins, a novel family of sarcomeric calcineurin-binding proteins. Proc. Natl. Acad. Sci. USA. 97:14632-14637 https://doi.org/10.1073/pnas.260501097
  5. Frey, N. and E. N. Olson. 2002. Calsarcin-3, a novel skeletal muscle-specific member of the calsarcin family, interacts with multiple Z-disc proteins. J. Biol. Chem. 277:13998-14004 https://doi.org/10.1074/jbc.M200712200
  6. Frey, N., T. Barrientos, J. M. Shelton, D. Frank, H. Rutten, D. Gehring, C. Kuhn, M. Lutz, B. Rothermel, R. Bassel-Duby, J. A. Richardson, H. A. Katus, J. A. Hill and E. N. Olson. 2004. Mice lacking calsarcin-1 are sensitized to calcineurin signaling and show accelerated cardiomyopathy in response to pathological biomechanical stress. Nat. Med. 10:1336-1343 https://doi.org/10.1038/nm1132
  7. Goureau, A., M. Yerle, A. Schmitz, J. Riquet, D. Milan, P. Pinton, G. Frelat and J. Gellin. 1996. Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36(2):252-262 https://doi.org/10.1006/geno.1996.0460
  8. Li, X. Y., B. Liu, B. Fan, M. Yu, M. J. Zhu, T. A. Xiong and K. Li. 2006. Mapping, tissue distribution and polymorphism study of the porcine SOCS2 and SOCS3 genes. Asian-Aust. J. Anim. Sci. 19:165-170
  9. Milan, D., R. Hawken, C. Cabau, S. Leroux, C. Genet, Y. Lahbib, G. Tosser, A. Robic, F. Hatey, L. Alexander, C. Beattie, B. Schook, M. Yerle and J. Gellin. 2000. IMpRH server: an RH mapping server available on the Web. Bioinformatics 16(6):558-559 https://doi.org/10.1093/bioinformatics/16.6.558
  10. Pan, P. W., S. H. Zhao, M. Yu, B. Liu, T. A. Xiong and K. Li. 2003. Identification of differentially expressed genes in the longissimus dorsi muscle tissue between duroc and erhualian pigs by mRNA differential display. Asian-Aust. J. Anim. Sci. 16:1066-1070 https://doi.org/10.5713/ajas.2003.1066
  11. Sonesson, A. K., K. H. de Greef and T. H. E. Meuwissen. 1998. Genetic parameters and trends of meat quality, carcass composition and performance traits in two selected lines of large white pigs. Livest. Prod. Sci. 57:23-32 https://doi.org/10.1016/S0301-6226(98)00163-8
  12. Schulz, R. A. and K. E. Yutzey. 2004. Calcineurin signaling and NFAT activation in cardiovascular and skeletal muscle development. Dev. Biol. 266:1-16 https://doi.org/10.1016/j.ydbio.2003.10.008
  13. Takada, F., D. L. Vander Woude, H. Q. Tong, T. G. Thompson, S. C. Watkins, L. M. Kunkel and A. H. Beggs. 2001. Myozenin: an alpha-actinin- and gamma-filamin-binding protein of skeletal muscle Z lines. Proc. Natl. Acad. Sci. USA. 98:1595-1600
  14. Wang, H., Z. Zhu, H. L. Wang, S. L. Yang, D. L. Mo and K. Li. 2006a. Characterization of different expression patterns of calsarcin-1 and calsarcin-2 in porcine muscle. Gene. 374:104-111 https://doi.org/10.1016/j.gene.2006.01.035
  15. Wang, H. L., H. Wang, Z. M. Zhu, S. L. Yang, S. T. Feng and K. Li. 2006b. Molecular characterization and expression patterns of porcine eukaryotic elongation factor 1A. Asian-Aust. J. Anim. Sci. 19:953-957 https://doi.org/10.5713/ajas.2006.953
  16. Wang, Y. F., M. Yu, M. F. te Pas, M. Yerle, B. Liu, B. Fan, T. A. Xiong and K. Li. 2004. Sequence characterization, polymorphism and chromosomal localizations of the porcine PSME1 and PSME2 genes. Anim. Genet. 35:361-366 https://doi.org/10.1111/j.1365-2052.2004.01176.x
  17. Yerle, M., P. Pinton, A. Robic, A. Alfonso, Y. Palvadeau, C. Delcros, R. Hawken, L. Alexander, C. Beattie, L. Schook, D. Milan and J. Gellin. 1998. Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet. Cell. Genet. 82:182-188 https://doi.org/10.1159/000015095

피인용 문헌

  1. Molecular characterization and association analysis of FBXO40 with partial hematological indexes in pig vol.37, pp.7, 2010, https://doi.org/10.1007/s11033-009-9928-1
  2. Gene vol.2017, pp.2314-6141, 2017, https://doi.org/10.1155/2017/5930918
  3. gene and their association with carcase and meat quality traits in chickens pp.1828-051X, 2018, https://doi.org/10.1080/1828051X.2018.1448307
  4. Tissue Distribution, SNP Detection and Association Study with Immune Traits of Porcine LBP and CD14 Genes vol.21, pp.8, 2008, https://doi.org/10.5713/ajas.2008.70689