Analysis of Relationship of the Korean Styracaceae by RAPD Markers

RAPD markers에 의한 한국산 때죽나무과(Styracaceae)식물의 유연관계

  • Published : 2007.06.30

Abstract

In order to estimate the relationships among four taxa (Styrax japonicus, Styrax obassia, Styrax shiraianus and Pterostyrax hispidus) of two genus in the Styracaceae and their regional populations, RAPDs analysis was performed. Fifteen random primers generating 238 RAPD bands were enough to reveal differences in genotype bands. They were clustered into three groups by the UPGMA phenogram. The first group was Styrax japonicus populations, the second group was consisted of Styrax obassia populations and S. shiraianus populations, and third group was Pterostyrax hispidus population. The UPGMA phenogram based on RAPD analyses showed that Styrax obassia and S. shiraianus had the closest relationship among four taxa. Species as well as genera of the Korean Styracaceae were well discriminated by the RAPD analyses.

한국산 때죽나무과 식물 2속 2분류군(때죽나무, 쪽동백나무, 좀쪽동백나무, 나래쪽동백)에 대한 속간, 종간 및 종내 지역별 개체군간의 유연관계를 조사하기 위하여 RAPD 분석을 수행하였다. 반응을 보인 15개의 random primer에서 238개의 유전자 표지밴드가 나타났으며, 이를 근거로 UPGMA Phenogram을 작성하였다. 4종의 개체군들은 3개의 유집군으로 묶이는 결과를 보였다. 첫 번째 유집군은 때죽나무 개체군, 두 번째 유집군은 쪽동백나무 개체군-좀쪽나무 개체군이 묶였고, 세 번째 유집군은 나래쪽동백 개체군으로 유집되었다. 취급된 분류군들 중 두 번째 유집군에 속하는 쪽동백나무-좀쪽동백나무가 가장 밀접한 유연관계를 갖는 것으로 밝혀졌다. 따라서 RAPD 분석으로 속과 종을 분류할 수 있었다.

Keywords

Acknowledgement

Supported by : 환경부, 과학기술부

References

  1. Baas, P. 1972. Anatomical contributions to plant taxonomy. 2. The affinities of Hua Pierre and Afrostyrax Perkins et Gilg, Blumea 20: 161-192
  2. Bentham, G. and J. D. Hooker. 1873. Genera Plantarum. Vol 2. Lovell Reeve, London. 1279 pp
  3. Copeland, H. F. 1938. The Styrax of northern California and the relationships of the Styracaceae. Amer. J. Bot. 25: 771-780 https://doi.org/10.2307/2436605
  4. Cronquist, A. 1981. An Integrated System of Classification of Flowering Plants. Columbia University Press, New York
  5. Dickinson, W. C. 1993. Floral anatomy of the Styracaceae, including observations on intra-ovarian trichomes. Bot J Linn Soc. 112: 223-255 https://doi.org/10.1111/j.1095-8339.1993.tb00318.x
  6. Dickinson, W. C. and K. D. Phend 1985. Wood anatomy of the Styracaceae: evolutionary and ecological considerations. International Association of Wood Anatomists Bulletin. 6: 3-22
  7. Doyle, J. J. and J. L. Doyle. 1987. A rapid DNA isolation methods for small quantities of fresh tissues. Phytochem, Bull. 19: 11-15
  8. Engler, A. and E. Gilg. 1924. Syllabus der Pflanzenfamilien. Borntraeger, Berlin
  9. ritsch, P. W. 2001. Phylogeny and biogeography of the flowering plant genus Styrax (Styracaceae) based on chloroplast DNA restriction sites and DNA sequences of the internal transcribed spacer region. Mol. Phylogenet. Evol. 19: 387-408 https://doi.org/10.1006/mpev.2001.0933
  10. Hemsley, W. B. and F. B. Forbes. 1889. Styraceae. Bot. J. Linn. Soc. 26: 75-77
  11. Heywood, V. H. 1985. Flowering Plants of the World. Prentice Hall, Englewood Cliffs
  12. Hutchinson, J. 1967. The Genera of Flowering. Vol 2. Clarendon, Oxford
  13. Hutchinson, J. 1973. The Families of Flowering Plants Arranged according to a New System Based on Their Probable Phylogeny. 3d ed. Clarendon, Oxford
  14. Hwang, S. and J. Grimes. 1996. Flora of China. 15: 253-271
  15. Lee, T. B. 1986. Illustrated Flora of Korea. Hyangmoonsa, Seoul (in Korean) Mori, T. 1921. Enumeration of Plants (in Japanese)
  16. Mori,T. 1921. Enumeration of Plants ( in Japanese)
  17. Morton, C. M. and W. C. Dickinson. 1992. Comparative pollen morphology of the Styracaceae. Grana 31: 1-15
  18. Nakai, T. 1911. Flora of Koreana. Journ, Coll. Sci. Univ. Tokyo 31: 85 (in Japanese)
  19. Nakai, T. 1911. 1938. Notule ad Plantas Asie Orientalis (V). J. Jap. Bot. 14: 2-3
  20. Nei, M. 1972. Genetic distance between populations. Amer. Nat. 106: 283-292 https://doi.org/10.1086/282771
  21. Nei, M. 1972.and W. H. Li. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273
  22. Palibin, J. W. 1900. Styracaceae Crataegoides. Conspectus Florae Koreae. Vol. 2
  23. Palser, B. F. 1961. Some aspects of embryology in the Ericales. In Recent Advances in Botany. Vol. 1: 685-689. University of Toronto Press. Toronto
  24. Perkins, J. 1907. Styracaceae. In A Engler, ed. Pflanzenreich IV, 241 (Heft 30). Engelmann, Leipzig
  25. Perkins, J. 1928. Ubersicht uber die gattungen der Styracaceae. Engelmann, Leipzig. p36
  26. Schadel, W. E. and W. C. Dickison 1979. Leaf anatomy and venation patterns of the Styracaceae. J. Arnold Arbor. 60: 8-37
  27. Steenis, C. G. G. J. and R. C. B. van der Brink. 1967. Miscellaneous botanical notes XVI. Bot Jahr. Syst. 86: 385-401
  28. Swofford, D. L. 2001. PAUP-Phylogenetic analysis using parsimony (and other mothods), ver. 4.0b8. Academic Press, Sunderland, MA
  29. Takhtajan, A. 1997. Diversity and Classification of Flowering Plants. Columbia University Press, New York
  30. Thorne, R. F. 1992. An updated phylogenetic classification of the flowering plants. Aliso 13: 365-389
  31. White, F. 1981. Lissocarpaceae. In Maguire, B. ed. The Botany of the Guayana Highland, pt 11. Mem. New York Bot. Gard. 32: 329-330
  32. Willis, J. C. 1937. A Dictionary of the Flowering Plants and Ferns. 8th ed. Cambridge University Press, Cambridge