Histamine-induced dilation of gastric glandular tubules of flounder, Paralichthys olivaceus

히스타민 유도 넙치(Paralichthys olivaceus) 위선부의 확장성 내강에 대한 조직학적 관찰

  • Ryu, Gab-Min (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University) ;
  • Song, Na-Young (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University) ;
  • Lee, Mu-Kun (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University) ;
  • Huh, Min-Do (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University)
  • 류갑민 (부경대학교 수산생명의학과) ;
  • 송나영 (부경대학교 수산생명의학과) ;
  • 이무근 (부경대학교 수산생명의학과) ;
  • 허민도 (부경대학교 수산생명의학과)
  • Published : 2007.04.30

Abstract

In order to differentiate the gastric tubular dilatation of olive flounder, Paralichthys olivaceus, from an atrophic change, a series of histological studies was carried out for normal gastric gland, naturally occurring and histamine-induced dilated glandular tubules. Normal glandular tubules were histologically quite similar to those already reported from other fish species. Naturally occurring, dilated gastric glandular tubules had the epithelial characteristics suffering from atrophy under light microscope, which was also the case in the histamine-induced gastric gland. But on semi-thin sections the area corresponding to the empty space of dilated lumina were actually shadowed with relatively bright area. Under transmission electron microscope this brightly shadowed area turned out to be an area tightly packed with abundant, slender cytoplasmic processes in both cases. These results strongly suggested that the dilative gastric glandular tubules under light microscope might be a morphological change indicating increased glandular activity rather than a pathologic atrophic change.

본 실험은 양식 넙치에서 임상적으로 빈번히 발생하는 위선세관의 확장성 변화가 위축성 변화인지를 알기 위하여 넙치의 정상 위선구조를 분명히 하는 한편 확장성 위선세관에 대하여 광학 및 전자현미경적 소견을 추구하고, 위액분비를 촉진하는 물질로 알려진 히스타민을 주사한 후 그 형태학적인 변화 내용을 서로 비교검토하였다. 외관상 건강한 넙치의 위선세관을 포함한 위선부의 조직학적 구조에 있어서는 이미 밝혀진 다른 어종과 큰 차이가 없었다. 광학현미경상에서 양식 넙치는 위선세관의 내강이 확장되고 소관 상피의 세포질의 양이 줄어든 위축성 변화를 보였고, 히스타민 주사 넙치에서도 유사한 소견을 보였다. 그러나 semi-thin 표본상에서 확장된 위선세관의 내강은 밝은 영역으로 나타났고, 이 밝은 영역은 주자전자현미경상에서 가늘고 긴 다수의 세포질 돌기로 이루어진 것임을 알 수 있었다. 따라서 광학현미경 하에서 관찰되는 위선세관의 확장성 변화는 위축성 병변이기 보다는 위액분비활동 증가 내지 과다에 따른 형태학적 변화의 하나인 것으로 추정되었다.

Keywords

References

  1. Bomgren, P., Einarsson, S. and Jonsson, A.-C.: Similarities and differences in oxynticopeptic cell ultrastructure of one marine teleost, Gadus morhua and one freshwater teleost, Oncorhynchus mykiss, during basal and histamine- stimulated phases of acid secretion. Fish Physiol. Biochem., 18: 285-296, 1998 https://doi.org/10.1023/A:1007791032472
  2. Cotran, R. S., Kumar, V. and Collins, T.: Pathologic Basis Of Disease. pp. 35-36. 6th ed., W. B. Saunders Company, USA, 1999
  3. Cross, P. C. and Mercer, K. L.: Cell and tissue ultrastructure: A functional perspective. pp. 2-206. W. H. Freeman and Company, New York, 1993
  4. Grau, A., Crespo, S., Sarasquete, M. C. and Gonzalez De Canales, M. L.: The digestive tract of the amberjack Seriola dumerili, Risso: a light and scanning electron microscope study. J. Fish BioI., 41: 287-303,1992 https://doi.org/10.1111/j.1095-8649.1992.tb02658.x
  5. Ito S. and Winchester R.J.:The fine structure of the gastric mucosa in the bat. J. Cell Biol., 16: 541-577,1963 https://doi.org/10.1083/jcb.16.3.541
  6. Konturek, P. C., Brozozowski, T., Kwiecien. S., Drozdowicz, D., Harsch, I. A., Meixner, H., Stachura, J., Hahn, E. G. and Konturek, S. J.: Effect of Helicobacter pylori on delay in ulcer healing induced by aspirin in rats. Eur. J. Phamacol., 451: 191-202, 2002 https://doi.org/10.1016/S0014-2999(02)02250-1
  7. Ling, E. A. and Tan, C. K.: Fine structure of the gastric epithelium of the coral fish, Chelmon rostratus (Cuvier). Okajimas Folia. Anat. Jap., 51: 285-310,1975 https://doi.org/10.2535/ofaj1936.51.6_285
  8. Mattisson, A. and Holstein, B.: The ultrastructure of the gastric glands and its relation to induced secretory activity of cod, Gadus morhua (Day). Acta. PhysioI. Scand., 109: 51-59, 1980 https://doi.org/10.1111/j.1748-1716.1980.tb06563.x
  9. Mobin, S. M. A., Kanai, K. and Yoshikoshi, K.: Vacuolar degeneration of gastric glands of juvenile Japanese flounder Paralichthys olivaceus. Fish. Sci., 65(6): 963-964, 1999 https://doi.org/10.2331/fishsci.65.963
  10. Mobin, S. M. A., Kanai, K. and Yoshikoshi, K.: Histopathological alterations in the digestive system of larval and juvenile Japanese flounder Paralichthys olivaceus reared on four feeding levels. J. Aquat. Anim. Health, 12: 196-208,2000 https://doi.org/10.1577/1548-8667(2000)012<0196:HAITDS>2.0.CO;2
  11. Morrison, C. M. and Wright Jr., J. R.: A study of the histology of the digestive tract of the Nile tilapia. J. Fish BioI., 54: 597-606, 1999 https://doi.org/10.1111/j.1095-8649.1999.tb00638.x
  12. Noaillac-Depeyre, J. and Gas, N.: Ultrastructural and cytochemical study of the gastric epithelium in a fresh water teleostean fish (Perea fluviatilis). Tiss. Cell 10: 23-37, 1978 https://doi.org/10.1016/0040-8166(78)90004-6
  13. Osman, A. H. K. and Caceci, T.: Histology of the stomach of Tilapia nilotica (Linnaeus, 1758) from the River Nile. J. Fish BioI., 38: 211-223, 1991 https://doi.org/10.1111/j.1095-8649.1991.tb03107.x
  14. Garrido, M. V. O., Oller, C. G. and Equisoain, M.A.A.: Effect of diet on gastric mucosa cells in the European eel (Anguilla anguilla L.) histochemical ultrastructural study. Micron, 27: 25-34, 1996 https://doi.org/10.1016/0968-4328(95)00031-3
  15. Peters, G.: The effect of stress on the stomach of the European eel, Anguilla anguilla L. J. Fish BioI., 21: 497-512,1982 https://doi.org/10.1111/j.1095-8649.1982.tb02855.x
  16. Scocco, P., Ceccarelli, P. and Menghi, G.: Glycohistochemistry of the Tilapia spp. stomach. J. Fish BioI., 49: 584-593, 1996
  17. Sedar, A. W.: The fine structure of the oxyntic cell in relation to the functional activity of the stomach. Ann. N Y Acad. Sci. USA 99: 929,1962
  18. Westeru, J.R.H. and Jennings, J. B.: Histochemical demonstration of hydrochloric acid in gastric tubules of teleosts using an in vivo Preussian blue technique. Comp. Biochem. Physiol., 35: 879-884, 1970 https://doi.org/10.1016/0010-406X(70)90083-6