Asian-Australasian Journal of Animal Sciences

  • 제20권4호
  • /
  • Pages.501-510
  • /
  • 2007
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Steroid Effects on Cell Proliferation, Differentiation and Steroid Receptor Gene Expression in Adult Bovine Satellite Cells

  • Lee, Eun Ju (Department of Biotechnology, Yeungnam University) ;
  • Choi, Jinho (Department of Biotechnology, Yeungnam University) ;
  • Hyun, Jin Hee (Department of Biotechnology, Yeungnam University) ;
  • Cho, Kyung-Hyun (School of Biotechnology, Yeungnam University) ;
  • Hwang, Inho (Department of Animal Resources and Biotechnology, Chonbuk National University) ;
  • Lee, Hyun-Jeong (National Livestock Research Institute) ;
  • Chang, Jongsoo (Korea National Open University) ;
  • Choi, Inho (Department of Biotechnology, Yeungnam University)
  • 투고 : 2006.08.08
  • 심사 : 2006.10.16
  • 발행 : 2007.04.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The present study was conducted to establish primary bovine muscle satellite cell (MSC) culture conditions and to investigate the effects of various steroid hormones on transcription of the genes involved in muscle cell proliferation and differentiation. Of three different types of proteases (type II collagenase, pronase and trypsin-EDTA) used to hydrolyze the myogenic satellite cells from muscle tissues, trypsin-EDTA treatment yielded the highest number of cells. The cells separated by hydrolysis with type II collagenase and incubated on gelatin-coated plates showed an enhanced cell attachment onto the culture plate and cell proliferation at an initial stage of cell growth. In this study, the bovine MSCs were maintained in vitro up to passage 16 without revealing any significant morphological change, and even to when the cells died at passage 21 with decreased or almost no cell growth or deformities. When the cells were incubated in a steroid-depleted environment (DMEM(-)/10% CDFBS (charcoal-dextran stripped FBS)), they grew slowly initially, and were widened and deformed. In addition, when the cells were transferred to an incubation medium containing steroid (DMEM(+)/10% FBS), the deformed cells resumed their growth and returned to a normal morphology, suggesting that steroid hormones are crucial in maintaining normal MSC morphology and growth. The results demonstrated that treatments with 19-nortestosterone and testosterone significantly increased AR gene expression (p<0.05), implying that both testosterone and 19-nortestosterone bind with AR and that the hormone bound-AR complex up-regulates the genes of its own receptor (AR) plus other genes involved in satellite cell growth and differentiation in bovine muscle.

키워드

참고문헌

  1. Allen, R. E. and L. L. Rankin. 1990. Regulation of satellite cell during skeletal muscle growth and development. PSEMB. 194:81-86.
  2. Allen, R. E., R. A. Merkel and R. B. Young. 1979. Cellular aspects of muscle growth: Myogenic cell proliferation. J. Anim. Sci. 49:115-127. https://doi.org/10.2527/jas1979.491115x
  3. Apple, J. K., M. E. Dikeman, D. D. Simms and G. Kuhl. 1991. Effects of synthetic hormone implants, singularly or in combinations, on performance, carcass traits, and longissimus muscle palatability of Holstein steers. J. Anim. Sci. 69:4437-4448. https://doi.org/10.2527/1991.69114437x
  4. Bischoff, R. 1974. Enzymatic liberation of myogenic cells from adult rat muscle. Anat. Rec. 180:645-662. https://doi.org/10.1002/ar.1091800410
  5. Bonavaud, S., O. Agbulut, G. D'Honneur, R. Nizard, V. Mouly and G. Butler-Browne. 2002. Preparation of isolated human muscle fibers: a technical report. In vitro Cell. Dev. Biol. Anim. 38:66-72. https://doi.org/10.1290/1071-2690(2002)038<0066:POIHMF>2.0.CO;2
  6. Campion, D. R. 1984. The muscle satellite cell: Int. Rev. Cytol. 87:225-251. https://doi.org/10.1016/S0074-7696(08)62444-4
  7. Chen, J. C. J. and D. J. Goldhamer. 2003. Skeletal muscle stem cells. Reproductive Biol. Endocrinol. 1:101-107. https://doi.org/10.1186/1477-7827-1-101
  8. Choi, I., L. J. Gudas and B. S. Katzenellenbogen. 2000. Regulation of keratin 19 gene expression by estrogen in human breast cancer cells and identification of the estrogen responsive gene region. Mol. Cell Endocrinol. 164:225-237. https://doi.org/10.1016/S0303-7207(00)00197-0
  9. Cornelison, D. D. and B. J. Wold. 1997. Single-cell analysis of regulatory gene expression in quiescent and activated mouse skeletal muscle satellite cells. Dev. Biol. 191:270-283. https://doi.org/10.1006/dbio.1997.8721
  10. Dodson, M. V., B. A. Mathison and B. D. Mathison. 1990. Effects of medium and substratum on ovine satellite cell attachment, proliferation and differentiation in vitro. Cell. Diff. Dev. 29:59-66. https://doi.org/10.1016/0922-3371(90)90024-Q
  11. Dodson, M. V., D. C. McFarland, A. L. Grant, M. E. Doumit and S. G. Velleman. 1996. Extrinsic regulation of domestic animalderived satellite cells. Dom. Anim. Endocrin. 13:107-126. https://doi.org/10.1016/0739-7240(95)00062-3
  12. Dodson, M. V., E. L. Martin, M. A. Brannon, B. A. Mathison and D. C. McFarland. 1987. Optimization of bovine satellite cellderived myotube formation in vitro. Tissue and Cell. 19:159-166. https://doi.org/10.1016/0040-8166(87)90001-2
  13. Dodson, M. V., D. C. McFarland, E. L. Martin and M. A. Brannon. 1986. Isolation of satellite cells from ovine skeletal muscle. J. Tiss. Cul. Meth. 10:233-237. https://doi.org/10.1007/BF01404483
  14. Doumit, M. E. and R. A. Merkel. 1992. Conditions for the isolation and culture of porcine myogenic satellite cells. Tissue and Cell. 24:253-262. https://doi.org/10.1016/0040-8166(92)90098-R
  15. Doumit, M. E., D. R. Cook and R. A. Merkel. 1996. Testosterone up-regulates androgen receptors and decreases differentiation of porcine myogenic satellite cells in vitro. Endocrinol. 137:1385-94. https://doi.org/10.1210/en.137.4.1385
  16. Duclos, M. J., B. Chevalier, C. Goddard and J. Simon. 1993. Regulation of amino acid transport and protein metabolism in myotubes derived from chicken muscle satellite cells by IGF-I. J. Cell. Physiol. 157:650-657. https://doi.org/10.1002/jcp.1041570327
  17. Greene, E. A. and R. H. Raub. 1992. Procedures for harvesting satellite cells from equine skeletal muscle. Equine. Nut. Phys. Soc. 12:33-35.
  18. Herschler, R. C., A. W. Olmsted, A. J. Edwards, R. L. Hale, T. Montgomery, R. L. Preston, S. J. Bartle and J. J. Sheldon. 1995. Production responses to various doses and ratios of estradiol benzoate and trenbolone acetate implants in steers and heifers. J. Anim. Sci. 73:2873-2881. https://doi.org/10.2527/1995.73102873x
  19. Hunt, D. W., D. M. Henricks, G. C. Skelley and L. M. Grimes. 1991. Use of trenbolone acetate and estradiol in intact and castrate male cattle: Effects on growth, serum hormones, and carcass characteristics. J. Anim. Sci. 69:2452-2462. https://doi.org/10.2527/1991.6962452x
  20. Johnson, B. J., P. T. Anderson, J. C. Meiske and W. R. Dayton. 1996. Effect of a combined trenbolone and estradiol implant on steroid hormone levels, feedlot performance, carcass characteristics and carcass composition of feedlot steers. J. Anim. Sci. 74:363-371. https://doi.org/10.2527/1996.742363x
  21. Johnson, B. J., N. Halstead, M. E. White, M. R. Hathaway and W. R. Dayton. 1998. Activation state of muscle satellite cells isolated from steers implanted with a combined trenbolone acetate and estradiol implant. J. Anim. Sci. 76:2779-2786. https://doi.org/10.2527/1998.76112779x
  22. Kahlert, S., C. Grohe, R. H. Karas, K. Lobbert, L. Neyses and H. Vetter. 1997. Effects of estrogen on skeletal myoblast growth. Biochem. Biophys. Res. Commun. 232:373-378. https://doi.org/10.1006/bbrc.1997.6223
  23. Kamanga-Sollo, E., M. S. Pampusch, G. Xi, M. E. White, M. R. Hathaway and W. R. Dayton. 2004. IGF-I mRNA levels in bovine satellite cell cultures: effects of fusion and anabolic steroid treatment. J. Cell Physiol. 201:181-189. https://doi.org/10.1002/jcp.20000
  24. Katzenellenbogen, B. S., I. Choi, R. Delage-Mourroux, T. R. Ediger, P. G. Martini, M. Montano, J. Sun, K. Weis and J. A. Katzenellenbogen. 2000. Molecular mechanisms of estrogen action: selective ligands and receptor pharmacology. J. Steroid Biochem. Mol. Biol. 74:279-285. https://doi.org/10.1016/S0960-0760(00)00104-7
  25. Lay, S. L., Lefrere, C. Trautwein, I. Dugail and S. Krief. 2002. Insulin and sterol-regulatory element-binding protein-1c (SREBP-1C) regulation of gene expression in 3T3-L1 adipocytes. J. Biol. Chem. 277:35625-35634. https://doi.org/10.1074/jbc.M203913200
  26. Lindquist, D. L. and P. A. de Alarcon. 1987. Charcoal-dextran treatment of fetal bovine serum removes an inhibitor of human CFU-megakaryocytes. Exp. Hematol. 15:234-238.
  27. McFarland, D. C., M. E. Doumit and R. D. Minshell. 1988. The turkey myogenic satellite cell: Optimization of in vitro proliferation and differentiation. Tissue and Cell. 20:899-908. https://doi.org/10.1016/0040-8166(88)90031-6
  28. Oh, Y. S., S. B. Cho, K. H. Beak and C. B. Choi. 2005. Effects of Testosterone, $17{\beta}$-estradiol, and Progesterone on the Differentiation of Bovine Intramuscular Adipocytes. Asian-Aust. J. Anim. Sci. 18:1589-1593. https://doi.org/10.5713/ajas.2005.1589
  29. Seale, P., L. A. Sabourin, A. Girgis-Gabardo, P. Gruss and M. A. Rudnicki. 2000. Pax7 is required for the specification of myogenic satellite cells. Cell. 102:777-786. https://doi.org/10.1016/S0092-8674(00)00066-0
  30. Shen, H. C. and G. A. Coetzee. 2005. The androgen receptor: unlocking the secrets of its unique transactivation domain. Vitam Horm. 71:301-319. https://doi.org/10.1016/S0083-6729(05)71010-4
  31. Sinha-Hikim, I., W. E. Taylor, N. F. Gonzalez-Cadavid, W. Zheng and S. Bhasin. 2004. Androgen receptor in human skeletal muscle and cultured muscle satellite cells: up-regulation by androgen treatment. J. Clin. Endocrinol. Metab. 89:5245-5255. https://doi.org/10.1210/jc.2004-0084
  32. Thompson, S. H., L. K. Boxhorn, W. Y. Kong and R. E. Allen. 1989. Trenbolone alters the responsiveness of skeletal muscle satellite cells to fibroblast growth factor and insulin-like growth factor I. Endocrinol. 124:2110-2117. https://doi.org/10.1210/endo-124-5-2110
  33. Tricarico, C., P. Pinzani, S. Bianchi, M. Paglierani, V. Distante, M. Pazzagli, S. A. Bustin and C. Orlando. 2002. Quantitative realtime reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies. Anal. Biochem. 309:293-300. https://doi.org/10.1016/S0003-2697(02)00311-1

피인용 문헌

  1. Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation vol.47, pp.7, 2011, https://doi.org/10.1007/s11626-011-9427-2
  2. Effects of Ectopic Expression of Transcription Factors on Adipogenic Transdifferentiation in Bovine Myoblasts vol.22, pp.10, 2012, https://doi.org/10.5352/JLS.2012.22.10.1316
  3. Effect of Gender-Specific Adult Bovine Serum on Gene Expression During Myogenesis vol.54, pp.3, 2012, https://doi.org/10.5187/JAST.2012.54.3.219
  4. Meeting the meat: delineating the molecular machinery of muscle development vol.58, pp.1, 2016, https://doi.org/10.1186/s40781-016-0100-x
  5. Effects of trypsinization and of a combined trypsin, collagenase, and DNase digestion on liberation and in vitro function of satellite cells isolated from juvenile porcine muscles vol.54, pp.6, 2018, https://doi.org/10.1007/s11626-018-0263-5
  6. Testicular Expression of Steroidogenic Enzyme Genes Is Related to a Transient Increase in Serum 19-nortestosterone during Neonatal Development in Pigs vol.20, pp.12, 2007, https://doi.org/10.5713/ajas.2007.1832
  7. Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition vol.51, pp.6, 2007, https://doi.org/10.5187/jast.2009.51.6.459
  8. 돼지 고기의 아나볼릭 스테로이드가 Myogenic Satellite Cell의 증식과 분화에 미치는 영향 vol.30, pp.5, 2007, https://doi.org/10.5851/kosfa.2010.30.5.842