Molecules and Cells

  • 제24권3호
  • /
  • Pages.441-444
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  • 2007
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Inhibition of DNA Methylation Is Involved in Transdifferentiation of Myoblasts into Smooth Muscle Cells

  • Lee, Won Jun (College of Health Science, Ewha Womans University) ;
  • Kim, Hye Jin (College of Health Science, Ewha Womans University)
  • 투고 : 2007.10.04
  • 심사 : 2007.10.08
  • 발행 : 2007.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

Despite the importance of cell fate decisions regulated by epigenetic programming, no experimental model has been available to study transdifferentiation from myoblasts to smooth muscle cells. In the present study, we show that myoblast cells can be induced to transdifferentiate into smooth muscle cells by modulating their epigenetic programming. The DNA methylation inhibitor, zubularine, induced the morphological transformation of C2C12 myoblasts into smooth muscle cells accompanied by de novo synthesis of smooth muscle markers such as smooth muscle ${\alpha}$-actin and transgelin. Furthermore, an increase of p21 and decrease of cyclinD1 mRNA were observed following zebularine treatment, pointing to inhibition of cell cycle progression. This system may provide a useful model for studying the early stages of smooth muscle cell differentiation.

키워드

과제정보

연구 과제 주관 기관 : Korean Research Foundation

참고문헌

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