Journal of Dental Rehabilitation and Applied Science (구강회복응용과학지)

Korean Academy of Stomatognathic Function and Occlusion (대한턱관절교합학회)
권호 표지

The Biocompatibility Evaluation of Resin-Based Root Canal Sealers

Resin-Based Root Canal Sealer의 생체 적합성 평가

  • Kim, Hyoung-Sun (Department of Dentistry, School of Dentistry, Chonbuk National University) ;
  • Chon, Seong-Min (Department of Dentistry, School of Dentistry, Chonbuk National University) ;
  • Moon, Jhong-Hyun (Department of Dentistry, School of Dentistry, Chonbuk National University) ;
  • Lee, Kwang-Won (Department of Dentistry, School of Dentistry, Chonbuk National University) ;
  • Yu, Mi-Kyung (Department of Dentistry, School of Dentistry, Chonbuk National University)
  • 김형선 (전북대학교 치과대학 치과보존학교실) ;
  • 전성민 (전북대학교 치과대학 치과보존학교실) ;
  • 문종현 (전북대학교 치과대학 치과보존학교실) ;
  • 이광원 (전북대학교 치과대학 치과보존학교실) ;
  • 유미경 (전북대학교 치과대학 치과보존학교실)
  • Published : 2007.03.30
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Abstract

I. Objective The primary requirement of an endodontic root canal sealer is the biologic compatibility, because they remain in close contact with living periapical tissues over a long period of time. The aim of this study was the evaluation of cytotoxicity and genotoxicity of resin-based root canal sealers, AH 26 and ADSEAL. II. Material & Methods In this study, human periodontal ligament cells, human oral cancer cells (KB) and mouse osteoblasts (MC-3T3-E1) were used. Specimens of AH26, ADSEAL were eluted with culture medium for 1, 3, 5 and 7 days. Cytotoxicity was evaluated by using tetrazolium bromide reduction assay (MTT assay) for mitochondrial enzyme activity and cell viability. Genotoxicity was evaluated by using alkaline single cell gel electrophoresis assay (Comet assay). Also cell apoptosis induced by AH 26 was detected by Hoechst33258 staining. III. Results AH 26 and ADSEAL exhibited cytotoxic effects in all investigated cell groups. Genotoxicity was also noted for both sealers in mouse osteoblasts (MC-3T3-E1). But, ADSEAL presented significantly low cytotoxicity and genotoxicity compared with AH 26. Cytotoxicity and genotoxicity induced by AH 26 resulted in apopotosis. IV. Conclusion Our results clearly indicate that the recently invented ADSEAL has better biocompatibility than another resin based root canal sealer, AH 26. However ideal root canal sealer should have not only biocompatibility but also satisfactory physico-chemical properties such as sealing ability and stability. Thus continuous studies and developments should follow.

Keywords

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