한국산업보건학회지 (Journal of Korean Society of Occupational and Environmental Hygiene)

  • 제17권3호
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  • Pages.235-244
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  • 2007
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  • 2384-132X(pISSN)
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  • 2289-0564(eISSN)
한국산업보건학회 (Korean Industrial Hygiene Association)
권호 표지

Styrene 노출에 반응을 보이는 혈청 단백질에 대한 프로테오믹스 분석

Proteomic analysis of serum proteins responsive to styrene exposure

  • 김기웅 (한국산업안전공단 산업안전보건연구원) ;
  • 허경화 (한국산업안전공단 산업안전보건연구원) ;
  • 원용림 (한국산업안전공단 산업안전보건연구원) ;
  • 정진욱 (한국산업안전공단 산업안전보건연구원) ;
  • 김태균 (한국산업안전공단 산업안전보건연구원) ;
  • 박인정 (한국산업안전공단 산업안전보건연구원)
  • Kim, Ki-Woong (Occupational Safety and Health Research Institute, KOSHA) ;
  • Heo, Kyung-Hwa (Occupational Safety and Health Research Institute, KOSHA) ;
  • Won, Yong Lim (Occupational Safety and Health Research Institute, KOSHA) ;
  • Jeong, Jin Wook (Occupational Safety and Health Research Institute, KOSHA) ;
  • Kim, Tae Gyun (Occupational Safety and Health Research Institute, KOSHA) ;
  • Park, Injeong (Occupational Safety and Health Research Institute, KOSHA)
  • 투고 : 2007.07.19
  • 심사 : 2007.09.11
  • 발행 : 2007.09.30
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초록

By comparing the proteins from the workers exposed to styrene with the ones from controls, it may be possible to identify proteins that play a role in the occurrence and progress of occupational disease and thus to study the molecular mechanisms of occupational disease. In order to find the biomarkers for assessing the styrene effects early, before clinical symptoms develop and to understand the mechanisms of adverse health effects, we surveyed 134 employees, among whom 52 workers(30 male and 22 female) were chronically exposed to styrene in 10 glass-reinforced plastic boat manufacturing factories in Korea and 82 controls had never been occupationally exposed to hazardous chemicals including styrene. The age and drinking habits and serum biochemistry such as total protein, BUN and serum creatinine in both groups were significantly different. Exposed workers were divided into three groups according to exposure levels of styrene(G1, below 1/2 TLV; G2, 1/2 TLV to TLV; G3, above TLV). The mean concentration of airborne styrene in G1 group was $10.93{\pm}11.33ppm$, and those of urinary mandelic acid(MA) and phenylglyoxylic acid(PGA) were $0.17{\pm}0.21$ and $0.13{\pm}0.11g/g$ creatinine, respectively. The mean concentration of airborne styrene in G2 and G3 groups were $47.54{\pm}22.43$ and $65.33{\pm}33.47ppm$, respectively, and levels of urinary metabolites such as MA and PGA increased considerably as expected with the increase in exposure level of styrene. The airborne styrene concentration were significantly correlated to the urinary concentration of MA(r=0.784, p=0.000) and PGA(r=0.626, p<0.001). In the 2D electrophoresis, the concentration of five proteins including complement C3 precursor, alpha-1-antitrypsin(AAT), vitamin D binding protein precursor(DBP), alpha-1-B-glycoprotein(A1BG) and inter alpha trypsin inhibitor(ITI) heavy chain-related protein were significantly altered in workers exposed to styrene compared with controls. While expression of complement C3 precursor and AAT increased by exposure to styrene, expression of DBP, A1BG and ITI heavy chain-related protein decreased. These results suggest that the exposure of styrene might affects levels of plasma proteinase, carriers of endogenous substances and immune system. In particular, increasing of AAT with the increase in exposure level of styrene can explain the tissue damage and inflammation by the imbalance of proteinase/antiproteinase and decrease of DBP, A1BG and ITI heavy chain-related protein in workers exposed to styrene is associated with dysfunction and/or declination in immune system and signal transduction

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