Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Comparison of Dipogenic and Osteogenic Potentials of Visceral and Subcutaneous Adipose Stem Cells of Human

인체 피하지방과 내장지방에서 유래한 줄기세포의 지방분화 및 골분화 능력 차이

  • Kang, Min Gu (Department of Plastic and Reconstructive Surgery, University of Ulsan College of Medicine) ;
  • Park, Jong Lim (Departments of Plastic and Reconstructive Surgery, Reserch Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Lee, Jin Hee (Departments of Plastic and Reconstructive Surgery, Reserch Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Chang, Hak (Departments of Plastic and Reconstructive Surgery, Reserch Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Minn, Kyung Won (Departments of Plastic and Reconstructive Surgery, Reserch Institute of Plastic and Reconstructive Surgery, Seoul National University College of Medicine) ;
  • Park, Gyu Ju (Departments of Surgery, Seoul National University College of Medicine)
  • 강민구 (울산대학교 의과대학 성형외과학교실) ;
  • 박종림 (서울대학교 의과대학 성형외과학교실. 성형재건연구소) ;
  • 이진희 (서울대학교 의과대학 성형외과학교실. 성형재건연구소) ;
  • 장학 (서울대학교 의과대학 성형외과학교실. 성형재건연구소) ;
  • 민경원 (서울대학교 의과대학 성형외과학교실. 성형재건연구소) ;
  • 박규주 (서울대학교 의과대학 외과학교실)
  • Received : 2007.01.08
  • Published : 2007.09.10
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Abstract

Purpose: Adipose tissue contains a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. It is well known that fat depots from different part of our body shows different nature not only in morphological aspect but also physiologic aspect. The authors compared the adipogenic potentials and osteogenic potentials of adipose stem cells from different anatomical sites of human. Methods: After laparotomy by surgery team, the authors isolated these adipose stem cells successfully from 7 men with an average age of 58, and induced differentiation along adipogenic and osteogenic lineages in vitro. On the 14th day, cells cultured in adipogenic media differentiated into adipocytes in vitro, as evidenced by positive Oil Red O staining of lipid vacuoles. On the 21st day, cells cultured in osteogenic media differentiated into osteoblasts in vitro as demonstrated by Alizarin red staining of a calcified extracellular matrix. Results: After exposure to adipogenic and osteogenic differentiation medium, subcutaneous adipose stem cells were found to possess greater adipogenic and osteogenic potentials than cells isolated from visceral adipose tissues. Conclusion: This study indicates that adipogenic and osteogenic potentials of adipose stem cells vary by their anatomical sites, with subcutaneous adipose stem cells exhibiting higher adipogenic and osteogenic potential than those isolated from visceral fat.

Keywords

References

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