한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제35권4호
  • /
  • Pages.347-351
  • /
  • 2007
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

제주도 감자 더뎅이병징에서 분리된 Streptomyces spp.의 16S rRNA 유전자 염기서열 분석

Phylogenetic Differentiation of Streptomyces spp. Isolated from Potato Scab Lesions in Jeju Island of Korea on the Basis of 16S rRNA Gene Sequences

  • 이수현 (제주대학교 의과대학 미생물학교실) ;
  • 고영환 (제주대학교 공과대학 식품생명공학과) ;
  • 김창진 (한국생명공학연구원) ;
  • 김범준 (서울대학교 의과대학 미생물학교실) ;
  • 이근화 (제주대학교 의과대학 미생물학교실)
  • Lee, Soo-Hyun (Department of Microbiology, Cheju National University College of Medicine) ;
  • Ko, Young-Hwan (Department of Food Science and Engineering, Cheju National University) ;
  • Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Bum-Joon (Department of Microbiology, Seoul National University College of Medicine) ;
  • Lee, Keun-Hwa (Department of Microbiology, Cheju National University College of Medicine)
  • 발행 : 2007.12.28
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초록

감자 더뎅이병은 제주도 전 지역에서 발생하는 병해로서 더뎅이병 발생 시 경제적인 손실이 막대한 것으로 알려져 있다. 본 연구에서는 제주도 감자 더뎅이병징이 있는 부위에서 Streptomyces spp.를 분리, 배양한 후, 16S rRNA 유전자를 이용하여 계통분석을 실시하였다. 계통분석 결과 제주도 감자 더뎅이병징이 있는 부위에서 분리된 균은 모두 Streptomyces spp.에 속하였으며, 대부분이 기존에 더뎅이병을 일으키는 Streptomyces spp.로 확인되었다. 그러나 일부의 분리균은 기존에 알려진 감자 더뎅이병원균과는 다르다. 따라서 이들 병원균에 대해서는 지방산과 단백질 분석, 그리고 DNA-DNA 혼성화 등과 같은 보다 많은 연구의 수행을 통하여 새로운 더뎅이병을 일으키는 Streptomyces spp.인지, 또는 아직까지 명명되지 않은 Streptomyces spp.인지를 확인해야 할 것으로 사료된다.

Potato scab is prevalent in all potato-growing areas of Jeju Island and causes economically significant losses. Streptomyces species are known as pathogens of potato scab. In this study, we analyzed the 16S rRNA sequences of Streptomyces spp, which are isolated from potato scab lesions in Jeju Island, and constructed 16S rRNA phylogenetic tree. All isolates were clearly differentiated into the genus Streptomyces, and the tree also showed that new scab-causing Streptomyces spp or not yet named species of Streptomyces are existed in Jeju Island, Korea.

키워드

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