Effects of Ionic Speciation of Lysine on Its Adsorption and Desorption Through a Sulfone-type Ion-Exchange Column

  • Choi, Dong-Hyouk (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Lee, Ki-Say (Department of Environmental Engineering and Biotechnology, Myongji University)
  • 발행 : 2007.09.30

초록

Lysine produced during microbial fermentation is usually recovered by an ion-exchange process, in which lysine is first converted to the cationic form (by lowering the pH to less than 2.0 with sulfuric acid) and then fed to a cationexchange column containing an exchanger that has a sulfone group with a weak counterion such as NH;. Ammonia water with a pH above 11 is then supplied to the column to displace the purified lysine from the column and allow its recovery. To enhance the adsorption capacity and for a possible reduction in chemical consumption, monovalent lysine fed at pH 4 was investigated in comparison with conventional divalent lysine fed at pH 1.5. The adsorption capacity increased by more than 70% on a mass basis using pH 4 feeding compared with pH 1.5 feeding. Lysine adsorbed at pH 4 started to elute earlier than that adsorbed at pH 1.5 when ammonia water was used as the eluant solution, and the extent of early elution became more notable at lower concentrations of ammonia. Moreover, the elution of monovalent lysine fed at pH 4 displayed a stiffer front boundary and higher peak concentration. However, when the ammonium concentration was greater than 2.0 N, complete saturation of the bed was delayed during adsorption and the percent recovery yield from elution was lowered., both drawbacks that were considered inevitable features originating from the increased adsorption of monovalent lysine.

키워드

참고문헌

  1. Atkinson, B. and F. Marvituna. 1991. Biochemical Engineering and Biotechnology Handbook, Chapter 20, 2nd Ed. Stockton Press, U.S.A
  2. Greenstein, J. P. and M. Winitz. 1984. Chemistry of Amino Acids, Vol. 1, pp. 486-487. Krieger, U.S.A
  3. Helfferich, F. 1962. Ion Exchange, Chapter 9. McGraw-Hill, NY, U.S.A
  4. Hsieh, C. L., K. P. Hsiung, and J. C. Su. 1995. Determination of lysine with ninhydrin-ferric reagent. Anal. Biochem. 224: 187-189 https://doi.org/10.1006/abio.1995.1027
  5. Jang, K. H. and M. L. Britz. 2005. Comparison of the cell surface barrier and enzymatic modification system in Brevibacterium fluvum and B. lactofermentum. Biotechnol. Bioprocess Eng. 10: 225-229 https://doi.org/10.1007/BF02932017
  6. Jeon, C. 2005. Mercury ion removal using a packed-bed column with granular aminated chitosan. J. Microbiol. Biotechnol. 15: 497-501
  7. Kawakita, T., Y. Ito, C. Sano, T. Ogura, and M. Saeki. 1991. Breakthrough curve of lysine on a column of a strong cationexchange resin of the ammonium form. Sep. Sci. Technol. 26: 619-635 https://doi.org/10.1080/01496399108049904
  8. Kim, H. M., R. Heinzle, and C. Wittmann. 2006. Degradation of aspartokinase by single nucleotide exchange leads to clobal flux rearrangement in the central metabolism of Corynebacterium glutamicum. J. Microbiol. Biotechnol. 16: 1174-1179
  9. Lanxess, Inc. Product Information: Lewatit S1468. http://www.lewatit.de/ion/en/products/ion_result.asp. (accessed 2007. 2. 19.)
  10. Lee, J. W., C. H. Lee, and Y. M. Koo. 2006. Sensitivity analysis of amino acids in simulated moving bed chromatography. Biotechnol. Bioprocess Eng. 11: 110-115 https://doi.org/10.1007/BF02931893
  11. Lee, K. and J. Hong. 1992. Electrokinetic transport of amino acids through a cation exchange membrane. J. Membr. Sci. 75: 107-120 https://doi.org/10.1016/0376-7388(92)80010-H
  12. Nagai, H. and G. Carta. 2004. Lysine adsorption on cation exchange resin. I. Ion exchange equilibrium and kinetics. Sep. Sci. Technol. 39: 3691-3710 https://doi.org/10.1081/SS-200041091
  13. Nagai, H. and G. Carta. 2004. Lysine adsorption on cation exchange resin. II. Column adsorption/desorption behavior and modeling. Sep. Sci. Technol. 39: 3711-3738 https://doi.org/10.1081/SS-200041093
  14. Nagai, H. and G. Carta. 2005. Lysine adsorption on cation exchange resin. III. Multicolumn adsorption/desorption operation. Sep. Sci. Technol. 40: 791-809 https://doi.org/10.1081/SS-200041099
  15. Park, D., Y. S. Yun, S. R. Lim, and J. M. Park. 2006. Kinetic analysis and mathematical modeling of Cr(VI) removal in a differential reactor packed with Ecklonia biomass. J. Microbiol. Biotechnol. 16: 1720-1727
  16. Prescott, S. C. and C. G. Dunn. 1959. Industrial Microbiology, Chapter 43, 3rd Ed. McGraw-Hill, U.S.A
  17. Rhee, H. K. and N. R. Amundson. 1982. Analysis of multicomponent separation by displacement development. AIChE J. 28: 423-433 https://doi.org/10.1002/aic.690280310
  18. Wankat, P. 1990. Rate-Controlled Separations, Chapter 6. Blackie, U.K
  19. Wendisch, V. F. 2006. Genetic regulation of Corynebacterium glutamicum metabolism. J. Microbiol. Biotechnol. 16: 999-1009
  20. Wu, D. J., Y. Xie, Z. Ma, and N. H. L. Wang. 1998. Design of simulated moving bed chromatography for amino acid separations. Ind. Eng. Chem. Res. 37: 4023-4035 https://doi.org/10.1021/ie9801711
  21. Yim, B. S. 2004. The Present and Future of Fermentation Industry. Technology Trend Report, KISTI, Korea