Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Antifungal Activities of Bacillus thuringiensis Isolates on Barley and Cucumber Powdery Mildews

  • Choi, Gyung-Ja (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung-Soo (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology) ;
  • Lee, Dong-Hyung (Research Institute of Environmental Microbiology, FIXON-BIO Co. Ltd.)
  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Fourteen Bacillus thuringiensis isolates having both insecticidal activity and in vitro antifungal activity were selected and tested for in vivo antifungal activity against tomato late blight, wheat leaf rust, tomato gray mold, and barley powdery mildew in growth chambers. All the isolates represented more than 70% disease control efficacy against at least one of four plant diseases. Specifically, 12 isolates exhibited strong control activity against barley powdery mildew. Under glasshouse conditions, four (50-02, 52-08, 52-16, and 52-18) of the isolates also displayed potent control efficacy against cucumber powdery mildew. To our knowledge, this is the first report of B. thuringiensis isolates that have disease control efficacy against powdery mildew of barley and cucumber as well as insecticidal activity.

Keywords

References

  1. Ahern, M., S. Verschueren, and D. van Sinderen. 2003. Isolation and characterization of a novel bacteriocin produced by Bacillus thuringiensis strain B439. FEMS Microbiol. Lett. 220: 127-131 https://doi.org/10.1016/S0378-1097(03)00086-7
  2. Carlton, B. C. 1993. Development of improved bioinsecticides based on Bacillus thuringiensis, pp. 258- 266. In S. O. Duke, J. J. Menn, and J. R. Plimmer (eds.), Pest Control with Enhanced Environmental Safety. American Chemical Society, Washington, D.C
  3. Cherif, A., S. Chehimi, F. Limem, B. M. Hansen, N. B. Hendriksen, D. Daffonchio, and A. Boudabous. 2003. Detection and characterization of the novel bacteriocin entomocin 9, and safety evaluation of its producer, Bacillus thuringiensis ssp. entomocidus HD9. J. Appl. Microbiol. 95: 990-1000 https://doi.org/10.1046/j.1365-2672.2003.02089.x
  4. Driss, F., M. Kallassy-Awad, N. Zouari, and S. Jaoua. 2005. Molecular characterization of a novel chitinase from Bacillus thuringiensis subsp. kurstaki. J. Appl. Microbiol. 93: 374- 379 https://doi.org/10.1046/j.1365-2672.2002.01693.x
  5. Falk, S. P., D. M. Gadoutry, P. Cortesi, R. C. Pearson, and R. C. Steem. 1995. Parasitism of Uncinula necator cleistothecia by the mycoparasite Ampelomyces quisqualis. Phytopathology 85: 794-800 https://doi.org/10.1094/Phyto-85-794
  6. Glare, T. R. and M. O'Callaghan. 2000. Bacillus thuringiensis: Biology, Ecology and Safety. John Wiley and Sons Ltd., Chichester, U.K
  7. H${\'{o}}$fte, H. and H. R. Whiteley. 1989. Insecticidal proteins of Bacillus thuringiensis. Microbiol. Rev. 53: 242-255
  8. Jung, H. K. and S.-D. Kim. 2005. An antifungal antibiotic purified from Bacillus megaterium KL39, a biocontrol agent of red-pepper Phytophthora-blight disease. J. Microbiol. Biotechnol. 15: 1001-1010
  9. Kim, J. S., J. Y. Choi, J. H. Chang, H. J. Shim, J. Y. Roh, B. R. Jin, and Y. H. Je. 2005. Characterization of an improved recombinant baculovirus producing polyhedra that contain Bacillus thuringiensis Cry1Ac crystal protein. J. Microbiol. Biotechnol. 15: 710-715
  10. Kim, J.-C., G. J. Choi, J.-H. Park, H. T. Kim, and K. Y. Cho. 2001. Activity against plant pathogenic fungi of phomalactone isolated from Nigrospora sphaerica. Pest Manag. Sci. 57: 554-559 https://doi.org/10.1002/ps.318
  11. Kim, P. I., H. Bai, H. Chae, S. Chung, Y. Kim, R. Park, and Y.-T. Chi. 2004. Purification and characterization of a lipopeptide produced by Bacillus thuringiensis CMB26. J. Appl. Microbiol. 97: 942-949 https://doi.org/10.1111/j.1365-2672.2004.02356.x
  12. Lecadet, M.-M., E. Frachon, V. Cosmao Dumanoir, H. Ripouteau, S. Hamon, P. Laurent, and I. Thiery. 1999. Updating of the H-antigen classification of Bacillus thuringiensis. J. Appl. Microbiol. 86: 660-672 https://doi.org/10.1046/j.1365-2672.1999.00710.x
  13. Lee, D.-H., I. H. Cha, D. S. Woo, and M. Ohba. 2003. Microbial ecology of Bacillus thuringiensis: Fecal populations recovered from wildlife in Korea. Can. J. Microbiol. 49: 465-471 https://doi.org/10.1139/w03-063
  14. Lee, H.-J., K.-H. Park, J.-H. Shim, R.-D. Park, Y.-W. Kim, J.-Y. Cho, H. HwangBo, Y.-C. Kim, G.-S. Cha, H. B. Krishnan, and K.-Y. Kim. 2005. Quantitative changes of plant defense enzymes in biocontrol of pepper (Capsicium annuum L.) late blight by antagonistic Bacillus subtilis HJ927. J. Microbiol. Biotechnol. 15: 1073-1079
  15. Lereclus, D., A. Delecluse, and M. Lecadet. 1993. Diversity of Bacillus thuringiensis toxins and genes, pp. 37-69. In P. F. Entwistle, J. S. Cory, M. J. Bailey, and S. Higgs (eds.), Bacillus thuringiensis, a Environmental Biopesticide: Theory and Practice. John Wiley & Sons Ltd., U.K
  16. Nofal, M. A. and W. M. Haggag. 2006. Integrated management of powdery mildew of mango in Egypt. Crop Prot. 25: 480- 486 https://doi.org/10.1016/j.cropro.2005.08.003
  17. Oh, S.-T., J.-K. Kim, S.-Y. Yang, and M.-D. Song. 2004. Characterization of Bacillus thuringiensis having insecticidal effects against larvae of Musca domestica. J. Microbiol. Biotechnol. 14: 1057-1062
  18. Ohba, M. and K. Aizawa. 1986. Distribution of Bacillus thuringiensis in soils of Japan. J. Invertebr. Pathol. 47: 277- 282 https://doi.org/10.1016/0022-2011(86)90097-2
  19. Paulitz, T. C. and R. R. Belanger. 2001. Biological control in greenhouse systems. Annu. Rev. Phytopathol. 39: 103-133 https://doi.org/10.1146/annurev.phyto.39.1.103
  20. Romero, D., A. Perez-Garcia, M. E. Rivera, F. M. Cazorla, and A. de Vicente. 2004. Isolation and evaluation of antagonistic bacteria towards the cucurbit powdery mildew fungus Podosphaera fusca. Appl. Microbiol. Biotechnol. 64: 263-269 https://doi.org/10.1007/s00253-003-1439-8
  21. Schnepf, E., N. Crickmore, J. Van Rie, D. Lereclus, J. Baum, J. Feitelson, D. R. Zeigler, and D. H. Dean. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62: 775-806
  22. Shisa, N., N. Wasano, A. Ohgushi, D.-H. Lee, and M. Ohba. 2002. Extremely high frequency of common flagellar antigens between Bacillus thuringiensis and Bacillus cereus. FEMS Microbiol. Lett. 213: 93-96 https://doi.org/10.1111/j.1574-6968.2002.tb11291.x
  23. Stabb, E. V., L. M. Jacobson, and J. Handelsman. 1994. Zwittermicin A-producing strains of Bacillus cereus from diverse soils. Appl. Environ. Microbiol. 60: 4404-4412
  24. Yarwood, G. E. 1957. Powdery mildews. Bot. Rev. 23: 235- 300 https://doi.org/10.1007/BF02872581