Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Application of Factorial Experimental Designs for Optimization of Cyclosporin A Production by Tolypocladium inflatum in Submerged Culture

  • Abdel-Fattah, Y.R. (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Enshasy, H. El (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications) ;
  • Anwar, M. (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Omar, H. (Microbiology Department, Faculty of Pharmacy, Alexandria University) ;
  • Abolmagd, E. (Bioprocess Development Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Applications)
  • Published : 2007.12.31
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Abstract

A sequential optimization strategy based on statistical experimental designs was employed to enhance the production of cyclosporin A (CyA) by Tolypocladium inflatum DSMZ 915 in a submerged culture. A 2-level Plackett-Burman design was used to screen the bioprocess parameters significantly influencing CyA production. Among the 11 variables tested, sucrose, ammonium sulfate, and soluble starch were selected, owing to their significant positive effect on CyA production. A response surface methodology (RSM) involving a 3-level Box-Behnken design was adopted to acquire the best process conditions. Thus, a polynomial model was created to correlate the relationship between the three variables and the CyA yield, and the optimal combination of the major media constituents for cyclosporin A production, evaluated using the nonlinear optimization algorithm of EXCEL-Solver, was as follows (g/l): sucrose, 20; starch, 20; and ammonium sulfate, 10. The predicted optimum CyA yield was 113 mg/l, which was 2-fold the amount obtained with the basal medium. Experimental verification of the predicted model resulted in a CyA yield of 110 mg/l, representing 97% of the theoretically calculated yield.

Keywords

References

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